Disease relevance of Endothelium, Corneal

• Expression of SV40 virus large T antigen by recombinant adenoviruses activates proliferation of corneal endothelium in vitro [1].
• Crim1 is also detected in other developing ocular tissues including corneal and conjunctival epithelia, corneal endothelium, retinal pigmented epithelium, ciliary and iridial retinae and ganglion cells [2].
• No patients in the group with intracameral cefazoline developed corneal endothelium or retinal toxicity [3].
• Intercellular adhesion molecule-1 on rat corneal endothelium in experimental uveitis [4].
• CONCLUSIONS: Together with cystic fibrosis transmembrane conductance regulator (CFTR) and calcium activated chloride channel-1 (CLCA1), these results bring the number of chloride channel genes known to be expressed in the corneal endothelium and epithelium to seven [5].

High impact information on Endothelium, Corneal

• Bovine and human corneal endothelial cells which expressed large T antigen proliferated and the characteristic morphologic features of corneal endothelium were maintained [1].
• The expression of P-cadherin was transient in many tissues, and its permanent expression was limited to certain tissues such as the epidermis, the mesothelium, and the corneal endothelium [6].
• The aqueous humor (AH) that fills the anterior chamber of the eye and bathes the corneal endothelial surface contains numerous immunomodulatory molecules, such as TGF-beta, which may protect the corneal endothelium from NK cell-mediated damage [7].
• Implantation of lens epithelia from older embryos showed that the lens epithelium maintained the ability to support the expression of N-cadherin and the formation of the corneal endothelium until E15 [8].
• PDGF-A is also present in the corneal endothelium anterior to the lens epithelium in embryonic and early postnatal eyes [9].

Chemical compound and disease context of Endothelium, Corneal

• PURPOSE: To evaluate the toxicity of a solution combining sodium hyaluronate 1.5% with lidocaine (0.5%, 1.0%, or 1.65%) to the rabbit corneal endothelium [10].
• Toxicity of Xylocaine to rabbit corneal endothelium [11].
• This study examined the effects on the structure and function of rabbit corneal endothelium of the low toxicity cryoprotectant glycerol [12].
• Dilution of the bupivacaine 1:1 with glutathione bicarbonate Ringer solution prevented corneal edema and damage to the corneal endothelium [13].
• As part of the development of methods of corneal cryostorage for transplantation, a toxicity study was carried out on the rabbit corneal endothelium using four cryoprotective additives (CPA's) 1) dimethyl sulphoxide (Me2SO), 2) propane-1,2-diol (PG), 3) glycerol (GLY), 4) polyvinylpyrrolidone (PVP) [14].

Biological context of Endothelium, Corneal

• A quantitative study of the relationship between cell size and keratan sulfate levels was conducted on normal bovine corneal endothelium [15].
• CONCLUSIONS: EDTA released cells from contact inhibition and promoted proliferation in corneal endothelium from older donors [16].
• Although the corneal endothelium is highly susceptible to Fas-induced apoptosis, this is apparently not the primary mechanism of CD4(+) T-cell-dependent rejection [17].
• Phagocytosis of polystyrene spheres in the rabbit corneal endothelium: contribution of lysosomal enzymes to the endothelial degeneration [18].
• The results suggest that agents that modulate receptors coupled to adenylate cyclase may effect the corneal endothelium by altering gene expression through the second messenger, cAMP [19].

Anatomical context of Endothelium, Corneal

• Cat corneal endothelium was damaged by a methylmethacrylate segment introduced into the anterior chamber [20].
• CONCLUSIONS: Nitric oxide can cause damage to the mitochondria, the most important energy source of the cell, and induce ultrastructural damage to the corneal endothelium and fibroblast [21].
• Viability of the corneal endothelium was excellent, except if corneas were incubated at high concentrations of chloroquine (0.5 mM) for prolonged periods (24 hr) [22].
• Vimentin stain was intensely localized in focal areas of the trabecular-meshwork cell, keratocytes, and throughout the corneal endothelium [23].
• Suppression of nuclear factor kappa B and CD18-mediated leukocyte adhesion to the corneal endothelium by dexamethasone [24].

Associations of Endothelium, Corneal with chemical compounds

• Sodium hyaluronate (HA) protects the corneal endothelium during cataract surgery [25].
• Sodium, potassium, two chloride cotransport in corneal endothelium: characterization and possible role in volume regulation and fluid transport [26].
• Relationship between fluid transport and in situ inhibition of Na(+)-K+ adenosine triphosphatase in corneal endothelium [27].
• Changes in density of the corneal endothelium were quantified by specular microscopy and counting of trypan-blue-stained cells [28].
• Effects of glutathione on the corneal endothelium were reexamined [29].

Gene context of Endothelium, Corneal

• In situ localization of bFGF receptors demonstrates that high affinity receptors for bFGF are present in corneal endothelium [30].
• 11 beta-HSD2 was restricted to the corneal endothelium [31].
• Corneal endothelium displayed specific immunological staining for CA I and CA II [32].
• Basic fibroblast growth factor: expression in cultured cells derived from corneal endothelium and lens epithelium [33].
• ICAM-1 and HLA-DR were expressed on keratocytes and the corneal endothelium in six of the seven specimens [34].

Analytical, diagnostic and therapeutic context of Endothelium, Corneal

• RT-PCR using calcium-activated chloride channel (CLCA)-specific primers was used to examine the expression of CLCA in the corneal endothelium [35].
• Perfusion of rabbit corneal endothelium with 0.5 mM chlorpromazine and exposure to ultraviolet (UV) light resulted in no change in total glutathione content [36].
• Using immunohistochemistry, the FP receptor protein was found in connective tissue fibroblasts, the corneal endothelium, and the vasculature; however, FP receptor expression was not detected using in situ hybridization [37].
• Immunolabeling techniques were used at the light, scanning, and transmission electron microscopic level to localize keratan sulfate on the corneal endothelium [15].
• As TNF is present in the aqueous humor during allograft rejection, and this is in contact with the corneal endothelium, this has the potential to restrict expression of a therapeutic gene to rejection episodes in the cornea [38].

References