Disease relevance of RGD1308492

• The alterations in oligodendrocytes in myelin basic protein-induced acute experimental autoimmune encephalomyelitis (EAE) in the Lewis rat were studied using the technique of pre-embedding immunolabelling with the Rip monoclonal antibody, which specifically labels the cytoplasm of the cell bodies and processes of oligodendrocytes [1].

High impact information on RGD1308492

• In parallel experiments, AMV were immunolabeled with the oligodendrocyte-specific monoclonal antibody Rip to provide information on oligodendrocyte development and the extent of myelination [2].
• The first oligodendrocytes to express MBP mRNA or Rip were promyelinating oligodendrocytes, which had a "star-burst" morphology and had not yet begun to form myelin sheaths [2].
• Additional antibodies used to characterize the rNSCs were markers for stem cells (nestin), immature neurons (betaIII-tubulin), astrocytes (glial fibrillary acidic protein [GFAP]), and oligodendrocytes (Rip) [3].
• This increase in NG2+ cell numbers did not reflect a nonspecific staining of reactive cells, as GFAP, OX-42, and Rip antibodies did not co-localise with NG2 + cells in double immunostained tissue sections [4].
• The distribution of Rip immunoreactivity coincides with that of myelinated axons in both the spinal cord and the cerebellum [5].

Biological context of RGD1308492

• At P9, AMV were immunohistochemically labeled with the Rip antibody, to enable analysis of the numbers of myelin sheaths and of promyelinating and myelinating oligodendrocytes; promyelinating oligodendrocytes are a recognisable immature phenotype which express myelin-related proteins prior to forming myelin sheaths [6].

Anatomical context of RGD1308492

• This paper introduces "Rip" a monoclonal antibody that produces relatively complete staining of oligodendrocytes and their processes in the adult central nervous system (CNS) [5].
• By 23 days after the insult, the subcortical white matter segregates into areas that contain ferritin-positive microglia and are devoid of Rip-positive oligodendrocytes or areas with Rip-positive cells and no ferritin-positive microglia [7].
• Oligodendrocytes in the rat hippocampus in situ and in organotypic slice cultures were studied by light and electron microscopic immunocytochemistry using the monoclonal Rip antibody [8].

Associations of RGD1308492 with chemical compounds

• In spinal cord sections of untreated and dexamethasone-treated rats with EAE, we found that apoptotic lymphocytes were phagocytosed by Rip-positive oligodendrocytes as well as by CD11b/c-positive macrophages/microglia and by astrocytes expressing glial fibrillary acidic protein [1].

Analytical, diagnostic and therapeutic context of RGD1308492

• Immunohistochemistry studies using Leu7, galactocerebroside, Rip and adenomatous polyposis coli tumor suppressor protein indicated that most apoptotic cells were cells of the oligodendroglial lineage regardless of the age of the animal [9].
• Myelinogenesis was investigated in whole-mounted anterior medullary vela from rats aged postnatal day (P) 10-12, using double immunofluorescence labelling with Rip and anti-neurofilament 200 (NF200) antibodies, to identify oligodendrocytes and axons, respectively [10].

References