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Gene Review

snu3  -  ncRNA

Schizosaccharomyces pombe 972h-

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Disease relevance of U3snRNA


High impact information on U3snRNA

  • Splicing of several genes is normal in these strains, ruling out an essential base pairing between U1 snRNA and 3' splice sites [2].
  • In mammalian cells, there are six major molecular species (U1 to U6 snRNA), which are complexed with proteins, forming small nuclear ribonucleoprotein particles, snRNPs [3].
  • There exist two regions of extended primary sequence identity between S. pombe and human U1 RNAs; the first comprises nucleotides involved in hydrogen bonding to 5' splice junctions, and the second is a single-stranded region which, in the human snRNA, forms part of the A protein binding site [4].
  • The 3'-end modification of the optimal synthetic substrate was identical to that observed with endogenous U6 snRNA isolated from HeLa cells [5].
  • Discovery of mRNA-type introns in the highly conserved region of the U6 snRNA genes led to the hypothesis that U6 snRNA functions as a catalytic element during pre-mRNA splicing [1].

Biological context of U3snRNA


Anatomical context of U3snRNA

  • Pre-mRNA processing takes place within the spliceosome, a dynamic structure composed of small nuclear RNA (snRNA) and proteins [9].

Other interactions of U3snRNA

  • A notable exception is a 19-bp block, centered 36 nucleotides upstream from the transcriptional start site, in which the two loci match in 15 positions; this motif may represent an RNA polymerase II upstream regulatory element, because related sequences are found preceding fission yeast U1, U2, U4, and U5 snRNA genes [8].
  • Rescue of the fission yeast snRNA synthesis mutant snm1 by overexpression of the double-strand-specific Pac1 ribonuclease [10].

Analytical, diagnostic and therapeutic context of U3snRNA

  • The complexity of the trimethylguanosine-capped, small nuclear RNA (snRNA) populations in a number of organisms has been examined using immunoprecipitation and two-dimensional gels [11].
  • The bank was screened by Northern blot analysis with probes complementary to S. pombe U6 small nuclear RNA (sn RNA), the gene for which has a splicesomal (mRNA-type) intron [12].


  1. Activity of chimeric RNAs of U6 snRNA and (-)sTRSV in the cleavage of a substrate RNA. Tani, T., Takahashi, Y., Ohshima, Y. Nucleic Acids Res. (1992) [Pubmed]
  2. 3' splice site recognition in S. cerevisiae does not require base pairing with U1 snRNA. Séraphin, B., Kandels-Lewis, S. Cell (1993) [Pubmed]
  3. The gene for the U6 small nuclear RNA in fission yeast has an intron. Tani, T., Ohshima, Y. Nature (1989) [Pubmed]
  4. U1 small nuclear RNA from Schizosaccharomyces pombe has unique and conserved features and is encoded by an essential single-copy gene. Porter, G., Brennwald, P., Wise, J.A. Mol. Cell. Biol. (1990) [Pubmed]
  5. A highly specific terminal uridylyl transferase modifies the 3'-end of U6 small nuclear RNA. Trippe, R., Sandrock, B., Benecke, B.J. Nucleic Acids Res. (1998) [Pubmed]
  6. Thirty-three nucleotides of 5' flanking sequence including the 'TATA' box are necessary and sufficient for efficient U2 snRNA transcription in Schizosaccharomyces pombe. Dandekar, T., Tollervey, D. Mol. Microbiol. (1991) [Pubmed]
  7. Structure analysis of the 5' external transcribed spacer of the precursor ribosomal RNA from Saccharomyces cerevisiae. Yeh, L.C., Lee, J.C. J. Mol. Biol. (1992) [Pubmed]
  8. The two similarly expressed genes encoding U3 snRNA in Schizosaccharomyces pombe lack introns. Selinger, D.A., Porter, G.L., Brennwald, P.J., Wise, J.A. Mol. Biol. Evol. (1992) [Pubmed]
  9. The evolutionary conservation of the splicing apparatus between fission yeast and man. Wentz-Hunter, K., Potashkin, J. Nucleic Acids Symp. Ser. (1995) [Pubmed]
  10. Rescue of the fission yeast snRNA synthesis mutant snm1 by overexpression of the double-strand-specific Pac1 ribonuclease. Rotondo, G., Gillespie, M., Frendewey, D. Mol. Gen. Genet. (1995) [Pubmed]
  11. High level of complexity of small nuclear RNAs in fungi and plants. Tollervey, D. J. Mol. Biol. (1987) [Pubmed]
  12. Isolation of novel pre-mRNA splicing mutants of Schizosaccharomyces pombe. Urushiyama, S., Tani, T., Ohshima, Y. Mol. Gen. Genet. (1996) [Pubmed]
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