Disease relevance of src

• Human immunodeficiency virus type 1 (HIV-1) NEF protein has been reported to share certain biochemical and structural properties with known oncoproteins like src or rats [1].

High impact information on src

• Expression of deregulated Xenopus fyn, a src-family member, resulted in a phenotype similar to that seen with deregulated src [2].
• However, in the fyn-injected oocytes, many more proteins were phosphorylated on tyrosine than in the src-injected oocytes [2].
• The Xenopus annexin II heavy chain lacks the highly conserved tyrosine at position 23 which is the site of src oncogene tyrosine kinase phosphorylation in the murine protein [3].
• The haploid genome of Xenopus laevis contains two src genes, and transcripts from both genes are found in the maternal RNA pool of the oocyte (Steele, R. E. (1985) Nucleic Acids Res. 13, 1747-1761) [4].
• Hybridization with probes specific for each of the two src RNAs indicates that the two genes are co-expressed in embryos and in at least some adult tissues as well as during oogenesis [4].

Biological context of src

• Using a reverse transcription/polymerase chain reaction assay, we demonstrate that expression of src+ mRNA is dependent on neural induction [5].
• Alternative splicing is used by the src protooncogene to derive two gene products, pp60c-src and pp60+. pp60+ is expressed exclusively in neurons, whereas pp60c-src is expressed in many tissues [5].
• Genomic and cDNA clones of the X. laevis src gene have been isolated and characterized by hybridization and DNA sequence analyses [6].
• The coding region of the gene is interrupted by 10 introns whose locations are identical to the introns in the coding regions of the src genes of human and chicken [7].
• We constructed in-frame deletion/replacement mutations in the Xenopus mos proto-oncogene that lie within conserved Mos-specific codons, but outside of the regions that are conserved among the src kinase family of genes [8].

Anatomical context of src

• The protein kinase C (PKC) activator 12-O-tetradecanoylphorbol-13-acetate (TPA) induces src+ mRNA expression in cultured stage 10+ animal cap ectoderm [5].
• The discovery that phosphotyrosines produced by receptor autophosphorylation or during substrate phosphorylation serve as an anchor for src homology 2 domains of several signaling proteins had a major impact on understanding how cytoplasmic enzymes are recruited at the level of the plasma membrane for subsequent activation [9].

Associations of src with chemical compounds

• TPA-dependent induction of src+ mRNA in ectoderm tissue is not blocked by cycloheximide and therefore is not dependent on protein synthesis [5].

Other interactions of src

• The catenin p120(ctn) (formerly p120cas) was first identified as a src- and receptor-protein tyrosine kinase substrate and later shown to interact directly with cadherins [10].

Analytical, diagnostic and therapeutic context of src

• By sequence analysis of genomic clones, the exon-intron structure of one of the two src genes from Xenopus laevis has been determined [7].

References