The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

Cp18  -  Chorion protein 18

Drosophila melanogaster

Synonyms: ACE3, CG6517, CP18, Chorion protein S18, Dmel\CG6517, ...
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

High impact information on Cp18

  • Two elements important for amplification of the third chromosome chorion gene cluster, ACE3 and Ori-beta, are directly bound by Orc (origin recognition complex), and two-dimensional gel analysis has revealed that the primary origin used is Ori-beta (refs 7-9) [1].
  • To demonstrate that the in vivo localization of DmORC is related to its DNA-binding properties, we find that purified DmORC binds to ACE3 and AER-d in vitro, and like its S. cerevisiae counterpart, this binding is dependent on ATP [2].
  • Drosophila ORC specifically binds to ACE3, an origin of DNA replication control element [2].
  • However, initiation was nonrandom; the majority of initiations appeared to occur near the Bgl II site located between the s18 and s15 chorion genes [3].
  • The role of ACE3 in Drosophila chorion gene amplification [4].
 

Biological context of Cp18

  • One long intergenic sequence, located in the distal 5' flanking region of gene s18, is homologous to ACE3, a major amplification control element and contains an 80-bp A/T-rich sequence, known to stimulate strong binding of the origin recognition complex (ORC) in D. melanogaster [5].
  • Therefore ACE3 does not appear to be analogous to a transcription enhancer [4].
  • We have characterized at the nucleotide level a 4.8-kilobase pair segment of the third chromosome of Drosophila melanogaster, which contains a cluster of three chorion genes, s18-1, s15-1 and s19-1 [6].
  • The highest number of dyad symmetries, reminiscent of sequences that function as viral replication origins, is found associated with the T- and A-rich regions between genes s18-1 and s15-1 [6].
  • Here we present the complete nucleotide sequence of this "amplification control element" and of genes encoding the chorion structural proteins s18-1 and s15-1, which are contained within it [7].
 

Anatomical context of Cp18

  • The parent construct supported 18- to 20-fold amplification, and contained the 320 bp ACE3, the approximately 1.2 kb S18 chorion gene and the 840 bp ori-beta [8].
  • We have used two types of deletions in the chorion cluster: the first was in vitro generated deletions of the ACE-3 region that were subsequently introduced into the germ line, and the second was deletions induced in vivo within a transposon at a preexisting chromosomal location, thus avoiding the complication of position effects [9].
  • Constructs containing chimeric combinations of Drosophila melanogaster and D. grimshawi DNA regions, as well as D. grimshawi sequences alone, can direct expression in the follicular epithelium, in an s18-specific temporal and spatial pattern [10].
 

Other interactions of Cp18

  • Despite marked temporal differences in their accumulation profiles, s36 and s18, putative chorion proteins, were similarly distributed throughout the floor, pillars, and roof of the endochorion [11].
  • Using immunolocalization, we observe that ACE3, a 440-bp chorion element that contains information sufficient to drive amplification, directs DmORC localization in follicle cells [2].

References

  1. Role for a Drosophila Myb-containing protein complex in site-specific DNA replication. Beall, E.L., Manak, J.R., Zhou, S., Bell, M., Lipsick, J.S., Botchan, M.R. Nature (2002) [Pubmed]
  2. Drosophila ORC specifically binds to ACE3, an origin of DNA replication control element. Austin, R.J., Orr-Weaver, T.L., Bell, S.P. Genes Dev. (1999) [Pubmed]
  3. Multiple replication origins are used during Drosophila chorion gene amplification. Heck, M.M., Spradling, A.C. J. Cell Biol. (1990) [Pubmed]
  4. The role of ACE3 in Drosophila chorion gene amplification. Orr-Weaver, T.L., Johnston, C.G., Spradling, A.C. EMBO J. (1989) [Pubmed]
  5. The chorion genes of the medfly. II. DNA sequence evolution of the autosomal chorion genes s18, s15, s19 and s16 in Diptera. Vlachou, D., Komitopoulou, K. Gene (2001) [Pubmed]
  6. Coding and potential regulatory sequences of a cluster of chorion genes in Drosophila melanogaster. Wong, Y.C., Pustell, J., Spoerel, N., Kafatos, F.C. Chromosoma (1985) [Pubmed]
  7. DNA sequence of a 3.8 kilobase pair region controlling Drosophila chorion gene amplification. Levine, J., Spradling, A. Chromosoma (1985) [Pubmed]
  8. Sequence requirements for function of the Drosophila chorion gene locus ACE3 replicator and ori-beta origin elements. Zhang, H., Tower, J. Development (2004) [Pubmed]
  9. Amplification-control element ACE-3 is important but not essential for autosomal chorion gene amplification. Swimmer, C., Delidakis, C., Kafatos, F.C. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  10. Positive and negative DNA elements of the Drosophila grimshawi s18 chorion gene assayed in Drosophila melanogaster. Swimmer, C., Kashevsky, H., Mao, G., Kafatos, F.C. Dev. Biol. (1992) [Pubmed]
  11. Eggshell assembly in Drosophila: processing and localization of vitelline membrane and chorion proteins. Pascucci, T., Perrino, J., Mahowald, A.P., Waring, G.L. Dev. Biol. (1996) [Pubmed]
 
WikiGenes - Universities