High impact information on CYP2D25

• Northern blot and reverse transcription-polymerase chain reaction experiments revealed that CYP2D25 mRNA is expressed in higher levels in liver than in kidney and in small amounts in adrenals, brain, heart, intestine, lung, muscle, spleen, and thymus [1].
• Purification and characterization of a vitamin D3 25-hydroxylase from pig liver microsomes [2].
• A monoclonal antibody raised against the vitamin D3 25-hydroxylase reacted strongly with the purified 25-hydroxylating cytochrome P-450 from pig kidney microsomes [Bergman & Postlind (1990) Biochem. J. 270, 345-350] [2].
• The 25-hydroxyvitamin D3 1 alpha-hydroxylase copurified with the vitamin D3 25-hydroxylase during purification [3].
• Isolation and properties of the CYP2D25 promoter: transcriptional regulation by vitamin D3 metabolites [4].

Biological context of CYP2D25

• The structure of vitamin D3 25-hydroxylase, as deduced by both DNA sequence analysis of the cDNA and protein sequence analysis, shows 70-80% identity with members of the CYP2D family [5].
• Transfection of the vitamin D3 25-hydroxylase cDNA into simian COS cells resulted in the synthesis of an enzyme that was recognized by a monoclonal antibody raised against purified vitamin D3 25-hydroxylase and catalyzed 25-hydroxylation in the bioactivation of vitamin D3 [5].
• The CYP2D25 promoter contains a putative vitamin D response element (VDRE) [4].
• The results from the current and previous studies on renal vitamin D hydroxylations imply that CYP2D25 has a biological role in kidney [6].
• The cytochrome P-450 showed the same apparent M(r), substrate specificity and N-terminal amino acid sequence as the previously purified vitamin D3 25-hydroxylase from pig liver microsomes [7].

Anatomical context of CYP2D25

• The results implicate that both CYP2D25 and CYP27A1 contribute to the 25-hydroxylation in hepatocytes and are important in the bioactivation of vitamin D(3) [8].
• Western blot analysis of microsomes from transformed yeast cells showed that both the wild-type and mutant CYP2D25 were expressed at comparable levels [9].

Associations of CYP2D25 with chemical compounds

• In addition to 25-hydroxylation, CYP2D25 also metabolizes the drug tolterodine [9].
• A monoclonal antibody raised against the vitamin D3 25-hydroxylase also recognized the vitamin D2 25-hydroxylase [7].

Analytical, diagnostic and therapeutic context of CYP2D25

• Northern blot analysis showed that the mRNA for vitamin D3 25-hydroxylase is found in liver and kidney [5].
• In this study, CYP2D25 was functionally expressed in the Saccharomyces cerevisiae W(R) strain and site-directed mutagenesis was used to study the role of substrate recognition site 3 (SRS-3) for the catalytic specificity of CYP2D25 [9].
• Immunohistochemistry experiments indicated that CYP2D25 is expressed almost exclusively in the cells of cortical proximal tubules [6].
• Genomic Southern blot analysis suggested the presence of a single gene for CYP2D25 in the pig [6].

References