The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

rpoC  -  DNA-directed RNA polymerase subunit beta'

Escherichia coli UTI89

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of rpoC


High impact information on rpoC

  • The terminator for the operon is located on a fragment which spans the 3' end of the rpoC gene [5].
  • In these plasmids trpR is positioned between the strong regulated tac promoter and the rpoC transcription terminator [6].
  • (3) The antiterminator had less effect on fragments containing Rho-independent terminators; the rpoC t fragment and three fragments derived from the rrnB terminator region stopped antiterminated transcription [7].
  • RfaH is also required for the transcription of wild-type levels of mRNA from promoter-distal genes in the rfaQ-K, traY-Z, and rplK-rpoC gene clusters, supporting the role for RfaH in transcriptional elongation [8].
  • This has now been confirmed in vitro where the lack of coordinate expression has been shown to be correlated with the selective inhibition of rpoB and rpoC gene expression by RNa polymerase [9].

Biological context of rpoC

  • The phenotype of the mutant was dependent on a mutation in the RNA polymerase gene rpoC [10].
  • The rpoC deletion and the point mutation cause over 20- and 10-fold reductions, respectively, in the copy number of ColE1 [11].

Analytical, diagnostic and therapeutic context of rpoC

  • The locations of four of the eleven rpoB amber mutations that are strongly polar on expression of the downstream rpoC gene have been determined through a combination of MaeI mapping, PCR amplification and DNA sequencing [12].


  1. Archaebacterial DNA-dependent RNA polymerases testify to the evolution of the eukaryotic nuclear genome. Pühler, G., Leffers, H., Gropp, F., Palm, P., Klenk, H.P., Lottspeich, F., Garrett, R.A., Zillig, W. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  2. Mutations in and monoclonal antibody binding to evolutionary hypervariable region of Escherichia coli RNA polymerase beta' subunit inhibit transcript cleavage and transcript elongation. Zakharova, N., Bass, I., Arsenieva, E., Nikiforov, V., Severinov, K. J. Biol. Chem. (1998) [Pubmed]
  3. Escherichia coli rpoC397 encodes a temperature-sensitive C-terminal frameshift in the beta' subunit of RNA polymerase that blocks growth of bacteriophage P2. Christie, G.E., Cale, S.B., Isaksson, L.A., Jin, D.J., Xu, M., Sauer, B., Calendar, R. J. Bacteriol. (1996) [Pubmed]
  4. Salmonella typhimurium rpoB and rpoC genes cloned on lambda phages. Tittawella, I.P. FEBS Lett. (1985) [Pubmed]
  5. Control features within the rplJL-rpoBC transcription unit of Escherichia coli. Barry, G., Squires, C.L., Squires, C. Proc. Natl. Acad. Sci. U.S.A. (1979) [Pubmed]
  6. High level production and rapid purification of the E. coli trp repressor. Paluh, J.L., Yanofsky, C. Nucleic Acids Res. (1986) [Pubmed]
  7. Antitermination of characterized transcriptional terminators by the Escherichia coli rrnG leader region. Albrechtsen, B., Squires, C.L., Li, S., Squires, C. J. Mol. Biol. (1990) [Pubmed]
  8. Enhancing transcription through the Escherichia coli hemolysin operon, hlyCABD: RfaH and upstream JUMPStart DNA sequences function together via a postinitiation mechanism. Leeds, J.A., Welch, R.A. J. Bacteriol. (1997) [Pubmed]
  9. Negative regulation of beta and beta' synthesis by RNA polymerase. Lang-Yang, H., Zubay, G. Mol. Gen. Genet. (1981) [Pubmed]
  10. Conditional change of DNA replication control in an RNA polymerase mutant of Escherichia coli. Rasmussen, K.V., Atlung, T., Kerszman, G., Hansen, G.E., Hansen, F.G. J. Bacteriol. (1983) [Pubmed]
  11. Origin-specific reduction of ColE1 plasmid copy number due to mutations in a distinct region of the Escherichia coli RNA polymerase. Ederth, J., Isaksson, L.A., Abdulkarim, F. Mol. Genet. Genomics (2002) [Pubmed]
  12. Physical mapping of a collection of Mael-generating amber mutations in the beta gene of Escherichia coli RNA polymerase and the functional effect of internal deletions constructed through their manipulation. Buyukuslu, N., Trigwell, S.M., Lim, P.P., Fujita, N., Ishihama, A., Ralphs, N.T., Glass, R.E. Genes Funct. (1997) [Pubmed]
WikiGenes - Universities