Disease relevance of Ccap

• The CCAP gene is expressed in every ganglion in each post-embryonic stage, except in the thoracic ganglia of first- and second-instar larvae [1].

High impact information on Ccap

• Immunohistochemical analyses in diverse insect species revealed the colocalization of pburs- and burs-immunoreactivity in some of the neurosecretory neurons that also express crustacean cardioactive peptide [2].
• Identification of G protein-coupled receptors for Drosophila PRXamide peptides, CCAP, corazonin, and AKH supports a theory of ligand-receptor coevolution [3].
• Effects on the execution of ecdysis and its circadian regulation indicate that CCAP is a key regulator of the behavior [4].
• In situ hybridization and immunocytochemistry revealed that bursicon is co-expressed with crustacean cardioactive peptide (CCAP) [5].
• This phenotype was mapped to the crustacean cardioactive peptide (CCAP)-neuropeptide circuit [6].

Biological context of Ccap

• We propose that EH activates both ventral central nervous system elements necessary for ecdysis (possibly the CCAP cells) and descending inhibitory neurons from the head [7].
• In contrast, we did not observe circadian changes in CCAP neuropeptide abundance in late pupae, although CCAP amounts were decreased in newly-emerged adults, presumably because the peptide is released at the time of ecdysis [8].
• The role of CCAP in adult cardiac function was studied in intact adult flies using targeted cell ablation and RNA interference (RNAi) [9].
• Amino acid sequence of CAP2b, an insect cardioacceleratory peptide from the tobacco hawkmoth Manduca sexta [10].
• We measured the effect of crustacean cardioactive peptide on Drosophila heart rate in the animal and in a tissue preparation [11].

Anatomical context of Ccap

• Here we present evidence that CCAP-expressing neurons (NCCAP) consist of two functionally distinct groups, one of which releases bursicon into the hemolymph and the other of which regulates its release [12].
• A cardioacceleratory peptide, CAP2b, identified originally in the lepidopteran Manduca sexta, stimulates fluid secretion by Malpighian tubules of the dipteran Drosophila melanogaster [13].

Associations of Ccap with chemical compounds

• Cardiac reversal was evoked pharmacologically by sequentially applying CCAP and glutamate to the heart [14].

Other interactions of Ccap

• Transgenic flies that lack CCAP neurons also lacked bursicon bioactivity [5].
• DNA corresponding to the corrected gene CG6111 was expressed in Chinese hamster ovary cells, where it was found to code for a receptor that could be activated by low concentrations of crustacean cardioactive peptide, which is a neuropeptide also known to occur in Drosophila and other insects (EC(50), 5.4 x 10(-10)M) [15].
• Molecular identification of a Drosophila G protein-coupled receptor specific for crustacean cardioactive peptide [15].

Analytical, diagnostic and therapeutic context of Ccap

• The spatial and temporal expression patterns of the CCAP gene in the M. sexta central nervous system were determined in all major post-embryonic stages using in situ hybridization techniques [1].

References