High impact information on 5-HT7

• We report the isolation of a cDNA coding for a Drosophila melanogaster serotonin receptor that displays a sequence, a gene organization, and pharmacological properties typical of the mammalian 5-HT2 serotonin receptor subtype [1].
• Although the physiology of 5-HT transmission in molluscs is well studied, little is known about the pharmacology and diversity of the 5-HT receptor system [2].
• Cloning and characterization of a Drosophila serotonin receptor that activates adenylate cyclase [3].
• Using a strategy based on nucleotide sequence homology between genes encoding receptors that interact with guanine nucleotide-binding proteins, we have isolated Drosophila genomic and cDNA clones encoding a functional serotonin receptor (5HT-dro receptor) [3].
• When stably introduced into mouse NIH 3T3 cells, the 5HT-dro receptor activates adenylate cyclase in response to serotonin and is inhibited by serotonin receptor antagonists such as dihydroergocryptine [3].

Biological context of 5-HT7

• The 5HT-dro receptor or closely related receptors might be responsible for the serotonin-sensitive cyclase that has been suggested to play a role in learning and modulation of circadian rhythm in a number of invertebrate systems [3].
• RESULTS: We generated or obtained flies with genetically altered expression of each of three Drosophila serotonin receptor subtypes (d5-HT1A, d5-HT1B, and d5-HT2) and assayed them for baseline sleep phenotypes [4].
• The first 88 amino acids of the 5HT receptor, isolated by a 5HT-affinity column followed by anion exchange chromatography and C3 reverse-phase HPLC exhibited a 43% sequence homology with Lymnaea stagnalis 5HT-receptor [5].
• An intron-containing gene encoding a novel human serotonin (5-HT) receptor was isolated from human genomic and cDNA libraries with probes directed to transmembrane regions of the adenylate cyclase stimulatory Drosophila serotonin receptor gene, 5-HTdrol [6].
• The deduced amino acid sequences of these planarian receptors, which are highly homologous to one another, show significant similarity to that of Drosophila 5HTdrol serotonin receptor [7].

Anatomical context of 5-HT7

• We previously reported that the serotonin receptor (5-ht(2Dro)) is expressed in the presumptive ectoderm with a pair-rule pattern [8].
• The quantity of the proteoglycan, DROP-1, obtained from Drosophila eggs and testes was also greatly improved by the guanidine hydrochloride extraction method [9].

Other interactions of 5-HT7

• Detection of electrophoretic variants of Notch, PS integrin, and DROP-1 proteins in Drosophila following extraction in guanidine hydrochloride [9].

Analytical, diagnostic and therapeutic context of 5-HT7

• Molecular cloning of a mammalian serotonin receptor that activates adenylate cyclase [10].

References