High impact information on trio

• We propose that distinct signals transduced via Trio and Dock act combinatorially to activate Pak in spatially restricted domains within the growth cone, thereby controlling the direction of axon extension [1].
• The guanine nucleotide exchange factor, Trio, is essential for Rac function in axon growth and guidance, but not for epithelial morphogenesis or myoblast fusion [2].
• In both Caenorhabditis elegans and Drosophila, UNC-73/Trio functions in axon guidance by signaling through the Rac GTPase to regulate cytoskeletal rearrangements necessary for growth cone migrations [3].
• The UNC-73/Trio RhoGEF-2 domain is required in separate isoforms for the regulation of pharynx pumping and normal neurotransmission in C. elegans [3].
• Genetic interactions implicate both Enabled and Trio in LAR signal transduction [4].

Biological context of trio

• In addition, tyrosine phosphorylation of Trio and Fra is elevated in S2 cells when Abl levels are increased [5].

Anatomical context of trio

• The Trio guanine nucleotide exchange factor functions in neural development in Caenorhabditis elegans and Drosophila and in the development of neural tissues and skeletal muscle in mouse [6].
• The Lar-interacting domain of Trio is constitutively tyrosine-phosphorylated when expressed in COS-7 cells and was highly phosphorylated when it was co-transfected with FAK [6].
• Thus, Trio is essential in order for neurites to faithfully extend on the correct pathways [7].

Other interactions of trio

• Here, we describe the identification and characterization of the Drosophila counterpart of Trio, a guanine nucleotide exchange factor (GEF) that associates with the receptor phosphatase LAR and regulates GTPase activation in vertebrate cells [8].

Analytical, diagnostic and therapeutic context of trio

• Fra interacts physically with Abl and Trio in GST-pulldown assays and in co-immunoprecipitation experiments [5].

References