High impact information on LEPR

• Detection and regulation of leptin receptor mRNA in ovine mammary epithelial cells during pregnancy and lactation [1].
• Furthermore, quantitative determinations, via ribonuclease protection assays, provided evidence that the level of leptin receptor expression was greatest during mid-pregnancy when active growth of the mammary gland is initiated [1].
• We measured expression of the leptin receptor (Ob-Rb) and the ghrelin/growth hormone releasing peptide receptor (GHS-R) in the arcuate nucleus of sheep held at either high or low levels of adiposity [2].
• Changes in expression of the genes for the leptin receptor and the growth hormone-releasing peptide/ghrelin receptor in the hypothalamic arcuate nucleus with long-term manipulation of adiposity by dietary means [2].
• We therefore examined in the fetal sheep hypothalamus during late gestation gene expression for leptin receptor (OB-Rb) and neuropeptides that regulate energy balance in the adult [3].

Biological context of LEPR

• Body composition, leptin, and the leptin receptor and their relationship to the growth hormone (GH) axis in growing wethers treated with zeranol [4].
• These data, compared with published data on leptin receptor gene expression, provide evidence that leptin could be produced by different cell types of the mammary gland and could act as a paracrine factor on mammary cell growth and differentiation via adipose-epithelial cells and myoepithelial-epithelial cell interactions [5].

Anatomical context of LEPR

• The long isoform (OB-Rb) is the major signaling form of the leptin receptor in the hypothalamus [6].

Associations of LEPR with chemical compounds

• Hypothalamic gene expression in sheep for cocaine- and amphetamine-regulated transcript, pro-opiomelanocortin, neuropeptide Y, agouti-related peptide and leptin receptor and responses to negative energy balance [7].
• Effect of cerebroventricular infusion of insulin and (or) glucose on hypothalamic expression of leptin receptor and pituitary secretion of LH in diet-restricted ewes [8].

Analytical, diagnostic and therapeutic context of LEPR

• We have used double-label immunofluorescence histochemistry to study the distribution of LR-like immunoreactivity (LR-ir) in the corticotropes, somatotropes, and gonadotropes of the ovine anterior pituitary [9].
• Location of the leptin receptors, as determined by in situ hybridization analysis, revealed that leptin receptor transcripts were expressed specifically in mammary epithelial cells [1].
• To ascertain the extent to which leptin may act on these neurons, we have used immunohistochemistry to examine co-localization of long-form of the leptin receptor (OB-Rb) and SRIF in the sheep hypothalamus [10].

References