Disease relevance of DDEFL1

• Isolation of development and differentiation enhancing factor-like 1 (DDEFL1) as a drug target for hepatocellular carcinomas [1].
• A new analytical strategy for biomarker recovery from directly coupled ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC Tof MS) data on biofluids is presented and exemplified using a study on hydrazine-induced liver toxicity [2].
• Metabonomics study of intestinal fistulas based on ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry (UPLC/Q-TOF MS) [3].
• A UPLC/Q-TOF MS-based metabonomics technique was employed to investigate sera from 40 patients with intestinal fistula and 17 healthy volunteers in an effort to find potential biomarkers of the disease and reveal their pathophysiological changes [3].

High impact information on DDEFL1

• This paper describes the development and partial validation of a fast, sensitive and specific ultra-performance liquid chromatography/tandem mass spectrometric (UPLC/MS/MS) method for the determination of testosterone (T) and its four metabolites, 6beta-OH-T, 16alpha-OH-T, 16beta-OH-T and 2alpha-OH-T, in in vitro samples [4].
• The UPLC system was coupled to the triple quadrupole Waters Micromass Quattro Premier [5].
• A simple, sensitive and rapid ultra-performance liquid chromatography/positive electrospray ionization tandem mass spectrometry (UPLC/ESI-MS/MS) method has been developed and validated for the determination of lercanidipine in human plasma [6].
• UPLC chromatograms showed very sharp peaks with less than 2 s wide at the base, except for alachlor [7].
• Furthermore, reduction of DDEFL1 expression by transfection of anti-sense S-oligonucleotides inhibited the growth of SNU475 cancer cells, in which DDEFL1 expression was highly up-regulated [1].

Biological context of DDEFL1

• METHODOLOGY: We performed bioinformatics analysis of whole genome expression profiling of muscle specimens and UPLC/MS based lipidomics analyses of plasma samples obtained in an earlier randomized trial from patients either on high dose simvastatin (80 mg), atorvastatin (40 mg), or placebo [8].

Associations of DDEFL1 with chemical compounds

• From among the transcripts that were commonly up-regulated in these tumors we identified a novel human gene at chromosomal band 1p36.13, termed DDEFL1 (development and differentiation enhancing factor-like 1), encoding a product that shared structural features with centaurin-family proteins [1].
• The differences in LC/MS performance were investigated by conducting a comparison of UPLC with another method previously optimized for HPLC-based separation and quantification of testosterone and its metabolites [4].
• The beta-blockers Oxprenolol, Metoprolol, Acebutolol, Atenolol, Propranolol, Pindolol, and Alprenolol were analysed by both UPLC/MS and HPLC/MS using mobile phases containing acetonitrile, TFA and either H2O or D2O [9].
• Sterols were separated on an Acquity UPLC BEH C18 column (100 mm x 1.0 mm, 1.7 microm particle size) with a gradient of methanol/water (1% acetonitrile) at a flow of 0.1 mL min(-1) [10].

Other interactions of DDEFL1

• We propose that ASAP3 functions nonredundantly with ASAP1 to control cell movement and may have a role in cancer cell invasion [11].

Analytical, diagnostic and therapeutic context of DDEFL1

• Using UPLC for separation resulted in 20% more detected components compared to HPLC [12].
• This study reported the application of LC-ESI/MS method to characterize O-diglycosyl flavanones of traditional Chinese medicine Fructus aurantii(Zhiqiao) and UPLC retention parameters method to expatiate the structure-retention relationship of these O-diglycosyl flavanones [13].

References