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Gene Review

PRPS2  -  phosphoribosyl pyrophosphate synthetase 2

Homo sapiens

Synonyms: PPRibP, PRS-II, Phosphoribosyl pyrophosphate synthase II, Ribose-phosphate pyrophosphokinase 2
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Disease relevance of PRPS2

  • The enzyme complex was then partially reconstituted in situ by coexpression of PAP39 with PRS I or PRS II in E. coli cells [1].

High impact information on PRPS2

  • PRSII was less sensitive to feedback inhibition by purine nucleotides and more sensitive to inhibition by 2,3-diphosphoglycerate than PRSI [2].
  • PRSI and PRSII also differed significantly in Km values for MgATP and ribose 5-phosphate, pH optima, and Mg2+ and Pi activation curves [2].
  • A cDNA clone of PRPS3 was sequenced and found to encode a predicted product of 317 amino acids which was highly homologous to those of PRPS1 and PRPS2 (94.3% and 91.2% identities, respectively) [3].
  • Human genes for PRS I and PRS II are localized at different regions on the X-chromosome and their promoter regions were examined [4].
  • Cloning of cDNA for the catalytic subunit revealed the presence of two highly homologous isoforms of 34 kDa, designated as PRS I and PRS II [4].

Biological context of PRPS2


Anatomical context of PRPS2

  • The 2.3 kb PRPS1 mRNA level was high in the brain and adrenal gland, whereas the 3.7 kb PRPS2 mRNA level prevailed in the lung and spleen [8].
  • Chloramphenicol acetyltransferase (CAT)/promoter fusion assays showed that a 2.0 kb region (human PRPS1) and a 1.1 kb region (human PRPS2) possessed the promoter activities in four cell lines [6].

Associations of PRPS2 with chemical compounds


Physical interactions of PRPS2

  • PAP39 complexed with PRS I was more readily degraded by proteolysis than seen with PRS II, in vivo and in vitro [1].

Other interactions of PRPS2

  • PRPS1 and PRPS2 loci mapped to the intervals Xq22-q24 and Xp22.2-p22.3, respectively, using a combination of in situ chromosomal hybridization and human x rodent somatic cell panel genomic DNA hybridization analyses [5].
  • Complementary DNA clones for phosphoribosylpyrophosphate synthetase subunits I and II (PRS I and PRS II) were used to determine the chromosomal localization of the corresponding human genes [9].


  1. Partial reconstitution of mammalian phosphoribosylpyrophosphate synthetase in Escherichia coli cells. Coexpression of catalytic subunits with the 39-kDa associated protein leads to formation of soluble multimeric complexes of various compositions. Ishijima, S., Asai, T., Kita, K., Sonoda, T., Tatibana, M. Biochim. Biophys. Acta (1997) [Pubmed]
  2. Overexpression, purification, and characterization of recombinant human 5-phosphoribosyl-1-pyrophosphate synthetase isozymes I and II. Nosal, J.M., Switzer, R.L., Becker, M.A. J. Biol. Chem. (1993) [Pubmed]
  3. A human testis-specific mRNA for phosphoribosylpyrophosphate synthetase that initiates from a non-AUG codon. Taira, M., Iizasa, T., Shimada, H., Kudoh, J., Shimizu, N., Tatibana, M. J. Biol. Chem. (1990) [Pubmed]
  4. Mammalian phosphoribosyl-pyrophosphate synthetase. Tatibana, M., Kita, K., Taira, M., Ishijima, S., Sonoda, T., Ishizuka, T., Iizasa, T., Ahmad, I. Adv. Enzyme Regul. (1995) [Pubmed]
  5. Cloning of cDNAs for human phosphoribosylpyrophosphate synthetases 1 and 2 and X chromosome localization of PRPS1 and PRPS2 genes. Becker, M.A., Heidler, S.A., Bell, G.I., Seino, S., Le Beau, M.M., Westbrook, C.A., Neuman, W., Shapiro, L.J., Mohandas, T.K., Roessler, B.J. Genomics (1990) [Pubmed]
  6. Promoter regions of the human X-linked housekeeping genes PRPS1 and PRPS2 encoding phosphoribosylpyrophosphate synthetase subunit I and II isoforms. Ishizuka, T., Iizasa, T., Taira, M., Ishijima, S., Sonoda, T., Shimada, H., Nagatake, N., Tatibana, M. Biochim. Biophys. Acta (1992) [Pubmed]
  7. Physical mapping of loci in the distal half of the short arm of the human X chromosome: implications for the spreading of X-chromosome inactivation. Wang, J.C., Passage, M.B., Ellison, J., Becker, M.A., Yen, P.H., Shapiro, L.J., Mohandas, T.K. Somat. Cell Mol. Genet. (1992) [Pubmed]
  8. Tissue-differential expression of two distinct genes for phosphoribosyl pyrophosphate synthetase and existence of the testis-specific transcript. Taira, M., Iizasa, T., Yamada, K., Shimada, H., Tatibana, M. Biochim. Biophys. Acta (1989) [Pubmed]
  9. Localization of human phosphoribosylpyrophosphate synthetase subunit I and II genes (PRPS1 and PRPS2) to different regions of the X chromosome and assignment of two PRPS1-related genes to autosomes. Taira, M., Kudoh, J., Minoshima, S., Iizasa, T., Shimada, H., Shimizu, Y., Tatibana, M., Shimizu, N. Somat. Cell Mol. Genet. (1989) [Pubmed]
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