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Gene Review

TAF2  -  TAF2 RNA polymerase II, TATA box binding...

Homo sapiens

Synonyms: 150 kDa cofactor of initiator function, CIF150, MRT40, RNA polymerase II TBP-associated factor subunit B, TAF(II)150, ...
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High impact information on TAF2

  • A comparison of different partial TBP-TAF assemblages established that a trimeric TBP-TAFII250-TAFII150 complex is minimally required for efficient utilization of the initiator and downstream promoter elements [1].
  • The analysis of a series of deletion mutants and chimeric receptors revealed the presence of two transcription activation functions (TAFs), located in the N-terminal region A/B (TAF-1) and the hormone binding domain (TAF-2) [2].
  • We defined a CIF150 (hTAF(II)150) consensus binding site and demonstrated that a CIF150-responsive cis element is present in the cyclin B1 core promoter [3].
  • The transient functional knockout of CIF150 (hTAF(II)150) protein led to cell cycle arrest at the G(2)/M transition in mammalian cell lines [3].
  • Here we present evidence that CIF150 (hTAF(II)150), the human homolog of Drosophila TAF(II)150, plays an important and selective role in establishing gene expression patterns necessary for progression through the cell cycle [3].

Biological context of TAF2

  • These sequences, transactivation function 1 (TAF1) in the amino terminus and TAF2 at the carboxyl terminus, display distinct transcriptional functions [4].
  • Human transcription factor hTAF(II)150 (CIF150) is involved in transcriptional regulation of cell cycle progression [3].
  • This phenomenon may be related to the interaction of TAF-2 in ligand bound receptor with the various regulators in the promoter region of specific estrogen responsive genes [5].

Anatomical context of TAF2


Associations of TAF2 with chemical compounds

  • Estrogen functions as an ER agonist by promoting functional synergism between TAF1 and TAF2 [4].
  • Alternatively, if a 'TAF2 function' is supplied by another factor, 4-OH tamoxifen can manifest ER agonist activity [4].
  • Like 17 beta-estradiol, OP is capable of stimulating the activity of both transcriptional activation functions, TAF-1 and TAF-2, in the receptor, as judged by analyzing the activity of the wild-type and mutant receptors in transiently transfected cells [7].

Other interactions of TAF2

  • Temperature-dependent DNA binding activity is also observed for TAF1-TAF2 heterodimers assembled with the ts13 mutant but not the wild-type TAF1 protein [8].
  • Consistent with this observation, the TAF2 function of the ER is not required on all promoters [9].
  • Novel cofactors and TFIIA mediate functional core promoter selectivity by the human TAFII150-containing TFIID complex [10].
  • Furthermore, we identified four other previously uncharacterized subunits of TFTC: hADA3, hTAFII150, hSPT3, and hPAF65beta [11].
  • However, in vitro binding assays revealed a specific and direct interaction between CIF150 and hTAF(II)135 [12].

Analytical, diagnostic and therapeutic context of TAF2

  • Here we describe the molecular cloning of CIF150, the human homolog of dTAF(II)150, and present biochemical evidence that this factor is involved in Inr activity [12].
  • Gel filtration experiments demonstrate that CIF150 (hTAF(II)150) seems to be less tightly associated with human transcription factor IID than hTAF(II)130 is associated with hTAF(II)250 [3].
  • PCR display analysis with the RNA derived from CIF150-depleted cells indicated that CIF150 (hTAF(II)150) is required for the transcription of only a subset of RNA polymerase II genes [3].


  1. Binding of TAFs to core elements directs promoter selectivity by RNA polymerase II. Verrijzer, C.P., Chen, J.L., Yokomori, K., Tjian, R. Cell (1995) [Pubmed]
  2. Agonistic and antagonistic activities of RU486 on the functions of the human progesterone receptor. Meyer, M.E., Pornon, A., Ji, J.W., Bocquel, M.T., Chambon, P., Gronemeyer, H. EMBO J. (1990) [Pubmed]
  3. Human transcription factor hTAF(II)150 (CIF150) is involved in transcriptional regulation of cell cycle progression. Martin, J., Halenbeck, R., Kaufmann, J. Mol. Cell. Biol. (1999) [Pubmed]
  4. Cellular mechanisms which distinguish between hormone- and antihormone-activated estrogen receptor. McDonnell, D.P., Dana, S.L., Hoener, P.A., Lieberman, B.A., Imhof, M.O., Stein, R.B. Ann. N. Y. Acad. Sci. (1995) [Pubmed]
  5. Effect of A-ring isomers of estradiol-17 beta on gene products in MCF-7 cells. VanderKuur, J.A., Brooks, S.C. Steroids (1994) [Pubmed]
  6. Identification of an estrogen response element upstream of the human c-fos gene that binds the estrogen receptor and the AP-1 transcription factor. Weisz, A., Rosales, R. Nucleic Acids Res. (1990) [Pubmed]
  7. Environmentally persistent alkylphenolic compounds are estrogenic. White, R., Jobling, S., Hoare, S.A., Sumpter, J.P., Parker, M.G. Endocrinology (1994) [Pubmed]
  8. Transcription factor IID recruitment and Sp1 activation. Dual function of TAF1 in cyclin D1 transcription. Hilton, T.L., Wang, E.H. J. Biol. Chem. (2003) [Pubmed]
  9. Human estrogen receptor transactivational capacity is determined by both cellular and promoter context and mediated by two functionally distinct intramolecular regions. Tzukerman, M.T., Esty, A., Santiso-Mere, D., Danielian, P., Parker, M.G., Stein, R.B., Pike, J.W., McDonnell, D.P. Mol. Endocrinol. (1994) [Pubmed]
  10. Novel cofactors and TFIIA mediate functional core promoter selectivity by the human TAFII150-containing TFIID complex. Martinez, E., Ge, H., Tao, Y., Yuan, C.X., Palhan, V., Roeder, R.G. Mol. Cell. Biol. (1998) [Pubmed]
  11. Identification of TATA-binding protein-free TAFII-containing complex subunits suggests a role in nucleosome acetylation and signal transduction. Brand, M., Yamamoto, K., Staub, A., Tora, L. J. Biol. Chem. (1999) [Pubmed]
  12. CIF150, a human cofactor for transcription factor IID-dependent initiator function. Kaufmann, J., Ahrens, K., Koop, R., Smale, S.T., Müller, R. Mol. Cell. Biol. (1998) [Pubmed]
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