High impact information on Cst13

• The cystatin-related epididymal spermatogenic (CRES) and recently identified testatin and cystatin T proteins define a new subgroup within the family 2 cystatins of cysteine protease inhibitors [1].
• The mouse cystatin T gene was mapped on a region of chromosome 2 that contains a cluster of cystatin genes, including cystatin C and CRES [2].
• Northern blot analysis demonstrated that expression of mouse cystatin T is highly restricted to the mouse testis [2].
• However, cystatin T lacks some of the conserved motifs believed to be important for inhibition of cysteine proteinase activity [2].
• The deduced amino acid sequence of cystatin T contains regions of significant sequence homology including the four highly conserved cysteine residues in exact alignment with all cystatin family 2 members [2].

Biological context of Cst13

• These results indicate that Cymg1 may play important roles in mouse spermatogenesis and sex maturation [3].
• Cymg1 is located in 2G3 of mouse chromosome 2 [3].
• We have cloned a novel gene, Cymg1 (GenBank accession number AY600990), from a mouse testis cDNA library [3].
• The Cymg1 expression level varied in different developmental stages: it was low 1 week postpartum, steadily increased 2 to 5 weeks postpartum, and was highest 7 weeks postpartum [4].
• By electrical hybridization, a novel gene, Cymg1 (GenBank accession No. AY600990), which has a full length of 0.78 kb, and contains four exons and three introns, was cloned from a mouse testis cDNA library [4].

Anatomical context of Cst13

• Cloning of a novel gene, Cymg1, related to family 2 cystatins and expressed at specific stages of mouse testis development [3].

Other interactions of Cst13

• Molecular cloning, chromosome mapping and characterization of a testis-specific cystatin-like cDNA, cystatin T [2].

Analytical, diagnostic and therapeutic context of Cst13

• Northern blot analysis showed that the Cymg1 is specifically expressed in adult mouse testes [4].
• RT-PCR also showed that Cymg1 was expressed in testis and spermatogonial cells [3].

References