Disease relevance of RNF103

• KF-1 monoclonal antibody defines a specific basement membrane antigen defect in dystrophic forms of epidermolysis bullosa [1].
• KF-1 monoclonal antibody is an IgG3 immunoglobulin with no binding to staphylococcal protein A. Two squamous carcinoma cell lines--namely, TR131 and TR146--quantitatively express the antigen [2].
• While bullous pemphigoid and KF-1 antigens were absent (N = 6 and N = 3, respectively) or significantly decreased (N = 4 and N = 7, respectively), epidermolysis bullosa acquisita antigen was routinely present though somewhat (N = 3) or moderately decreased (N = 3) [3].
• Prenatal diagnosis of dominant and recessive dystrophic epidermolysis bullosa: application and limitations in the use of KF-1 and LH 7:2 monoclonal antibodies and immunofluorescence mapping technique [4].
• Burkholderia cepacia KF1, isolated from a pneumonia patient, produces a 37-kDa extracellular metalloprotease [5].

High impact information on RNF103

• This study has demonstrated antigenic alterations within each ultrastructural subregion of papulonodular basal cell carcinoma tumor nest basement membranes by identifying the virtual absence of C3d,g (sublamina densa) as well as a significant reduction in KF-1 (lamina densa) and bullous pemphigoid (lamina lucida) antigens [3].
• Prenatal diagnosis is now possible for junctional and recessive dystrophic forms of epidermolysis bullosa (EB); however, there is no similar published experience for dominant dystrophic EB, although data with KF-1 monoclonal antibody suggests that both forms of dystrophic EB can be identified at least postnatally with this unique probe [4].
• Biochemical characterization of the epithelial basement membrane antigen defined by the monoclonal antibody KF-1 [2].
• KF-1 antigen is first expressed when the epidermis is further stratified, hemidesmosomes and anchoring fibrils are present in greater numbers and with increased frequency at the dermal-epidermal junction, and hair follicles have begun to bud downward from the basal layer of the epidermis [6].
• Immunoelectron microscopic studies of normal human skin and esophagus showed specific binding of KF-1 to the lamina densa of the BMZ, a localization identical to that of type IV collagen [7].

Chemical compound and disease context of RNF103

• We employed the recent development of surface-enhanced laser desorption/ionization ProteinChip technology to measure protein expression in three human ovarian cancer cell lines (KF-1, MN-1, and A2780) and their sublines (KF-r, MN-r, and A2780cp) resistant to cisplatin [8].

Biological context of RNF103

• We propose that KF-1 is involved in membranous protein-sorting apparatus similarly to RAPsyn [9].
• ADCC activity of human leukocytes was enhanced by KF1, KF2, KF3, DA and NE at 1.0 microgram/mL concentrations [10].
• Possible UVC-protective properties of CA, a plant phenolic compound with antioxidant activity, were investigated on human KF1 diploid fibroblast and A431 epidermoid carcinoma cell lines [11].

Analytical, diagnostic and therapeutic context of RNF103

• We now report our experience with light microscopy, electron microscopy, immunofluorescence mapping, and KF-1 and LH 7:2 monoclonal antibodies, in both a mother with dominant dystrophic EB and her fetus at risk, and in a fetus previously shown to be affected with recessive dystrophic EB [4].

References