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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Antibody-Dependent Cell Cytotoxicity

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Disease relevance of Antibody-Dependent Cell Cytotoxicity


High impact information on Antibody-Dependent Cell Cytotoxicity

  • Here we show that a CD4 immunoadhesin can mediate antibody-dependent cell-mediated cytotoxicity (ADCC) towards HIV-infected cells, although, unlike natural anti-gp120 antibodies, it does not allow ADCC towards uninfected CD4-expressing cells that have bound soluble gp120 to the CD4 on their surface [6].
  • Anti-CD16 antibodies block natural killer-cell mediated antibody dependent cellular cytotoxicity (ADCC) [7].
  • Our data also suggest that IFN-gamma is a potent stimulator of both NK and ADCC activity [8].
  • 2d of pretreatment was optimal for enhancing ADCC. rIL-4 was inactive and IL-4-depleted Con A-induced spleen lymphokine retained its ADCC-stimulating activity [9].
  • Although these cells failed to mediate significant levels of NK cell-mediated cytotoxicity even after stimulation with IL-2, they efficiently functioned as effectors of antibody-dependent cellular cytotoxicity (ADCC) [10].

Chemical compound and disease context of Antibody-Dependent Cell Cytotoxicity


Biological context of Antibody-Dependent Cell Cytotoxicity


Anatomical context of Antibody-Dependent Cell Cytotoxicity

  • We identified the effector cells in human PBL that mediate IgG2a or IgG2b ADCC as nonadherent killer (K) cells [20].
  • Further purification of the null cell population by filtration over nylon wool columns to reduce the number of contaminating latex ingesting monocytes did not reduce ADCC effector activity [21].
  • Because a single SLE plasma could induce ADCC in T cells from several different unrelated individuals, it is unlikely that antibodies directed against particular human leukocyte antigens (HLA) or blood group antigens are involved [22].
  • Monocytes and macrophages were able to utilize the chimeric IgG1 and, to a lesser degree, IgG4 and IgG3 antibodies to lyse tumor-cell targets in ADCC assays [23].
  • Neutrophil Lym-1-dependent cytotoxicity (ADCC) in the absence of IFN-gamma was weak and inconsistent [17].

Associations of Antibody-Dependent Cell Cytotoxicity with chemical compounds

  • In addition, C3-fragment-bearing cells inhibited the lysis of antibody-coated cells not concluded that target cell bound C3 fragments enhance ADCC by improving contact between target cells and those effector cells which have C3 receptors [24].
  • ADCC activity was found primarily in the plastic nonadherent cell population and was greatly enriched following removal of phagocytic cells by carbonyl iron [25].
  • In fact, CGD PMN had an ADCC against BCNU-treated Raji cells lower than that mediated by normal PMN, but comparable to that observed with untreated targets, and phenylbutazone reduced the lysis of BCNU-treated Raji cells by normal PMN to the level observed with the use of untreated targets [1].
  • In vivo inoculations of high doses of hydrocortisone resulted in a reduction of NK activity, but ADCC was not affected [26].
  • The inhibition of the Raji cell glutathione redox cycle by carmustine [1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU)] increased cell susceptibility to PMN-mediated ADCC [1].

Gene context of Antibody-Dependent Cell Cytotoxicity

  • On the other hand, PMN-mediated Lym-1 ADCC was induced or significantly augmented by various cytokines, such as GM-CSF, TNFalpha, and gamma-IFN, and chemotaxins, such as formyl peptides (FMLP), complement fragment C5a, and IL-8 [27].
  • The functional activity of Fc gamma RI on PMN during G-CSF therapy was shown by measuring binding of monomeric human IgG and antibody-dependent cellular cytotoxicity (ADCC) [28].
  • The resistance of Crry-expressing tumor cells to NK cell-mediated ADCC could be reversed by treatment with anti-Crry F(ab)(2) [29].
  • ADCC was not prevented by inhibitory effects mediated by HLA class I. We propose that treatment with chimeric CD19 antibodies leading to ADCC by donor-derived NK cells may become a therapeutic option for the post-transplantation treatment of minimal residual B-lineage ALLs [30].
  • Here we report that a chain-termination mutation in CRYBB2 is associated with ADCC in this family [31].

Analytical, diagnostic and therapeutic context of Antibody-Dependent Cell Cytotoxicity

  • This was also done for the combination of the ADCC assay results plus the A or B antigen density and the direct antiglobulin test plus the titre of maternal IgG anti-A or anti-B antibodies [32].
  • The resulting immunocytokine, DI-Leu16-IL-2, retained full anti-CD20 activity as assessed by fluorescence-activated cell-sorting (FACS) analysis, and had enhanced antibody-dependent cellular cytotoxicity (ADCC) effector function relative to the DI-Leu16 antibody or control anti-CD20 antibody (rituximab) [33].
  • Although decreased ADCC activity of PBMCs was observed in patients who responded poorly to conventional chemotherapy, it could be significantly augmented by the stimulation with interleukin-2 (IL-2), IL-12, or IL-15 [34].
  • These results suggest that lymphokines such as IL-1 and TNF may synergize with IL-2 in the induction of ADCC and could thus potentially be useful for the immunotherapy of established tumors when combined with the administration of specific anti-tumor antibodies [35].
  • These data indicate that NKR-P1bright/L.E.bright and NKR-P1bright/L.E.dim subpopulations of rat NK cells have different capacities for: 1) triggering through NKR-P1; and 2) E-A rosette formation and lysis of antibody-sensitized target cells by ADCC [36].


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  2. Strong antitumor activities of IgG3 antibodies to a human melanoma-associated ganglioside. Hellström, I., Brankovan, V., Hellström, K.E. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  3. Tumor-specific loss of 11p15.5 alleles in del11p13 Wilms tumor and in familial adrenocortical carcinoma. Henry, I., Grandjouan, S., Couillin, P., Barichard, F., Huerre-Jeanpierre, C., Glaser, T., Philip, T., Lenoir, G., Chaussain, J.L., Junien, C. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
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  5. Impaired antibody-dependent cellular cytotoxicity mediated by herceptin in patients with gastric cancer. Kono, K., Takahashi, A., Ichihara, F., Sugai, H., Fujii, H., Matsumoto, Y. Cancer Res. (2002) [Pubmed]
  6. Biological properties of a CD4 immunoadhesin. Byrn, R.A., Mordenti, J., Lucas, C., Smith, D., Marsters, S.A., Johnson, J.S., Cossum, P., Chamow, S.M., Wurm, F.M., Gregory, T. Nature (1990) [Pubmed]
  7. The Fc gamma receptor of natural killer cells is a phospholipid-linked membrane protein. Simmons, D., Seed, B. Nature (1988) [Pubmed]
  8. gamma-Interferon induced by S. aureus protein A augments natural killing and ADCC. Catalona, W.J., Ratliff, T.L., McCool, R.E. Nature (1981) [Pubmed]
  9. Role of interleukin 2, interleukin 4, and alpha, beta, and gamma interferon in stimulating macrophage antibody-dependent tumoricidal activity. Ralph, P., Nakoinz, I., Rennick, D. J. Exp. Med. (1988) [Pubmed]
  10. Functional properties of a unique subset of cytotoxic CD3+ T lymphocytes that express Fc receptors for IgG (CD16/Leu-11 antigen). Lanier, L.L., Kipps, T.J., Phillips, J.H. J. Exp. Med. (1985) [Pubmed]
  11. Delineation of type-specific regions on the envelope glycoproteins of human T cell leukemia viruses. Chen, Y.M., Zhang, X.Q., Dahl, C.E., Samuel, K.P., Schooley, R.T., Essex, M., Papas, T.S. J. Immunol. (1991) [Pubmed]
  12. The effect of prednisolone on antibody-dependent cell-mediated cytotoxicity and the growth of type I herpes simplex virus in human cells. Cooper, J.A., Daniels, C.A., Trofatter, E.F. Invest. Ophthalmol. Vis. Sci. (1978) [Pubmed]
  13. Antibody-dependent cellular cytotoxicity against drug-induced antigens in L5178Y mouse lymphoma. Franco, P., Veronese, F., Levi, F., Goldin, A., Nicolin, A. Br. J. Cancer (1982) [Pubmed]
  14. The possible mode of escape of adult T-cell leukaemia cells from antibody-dependent cellular cytotoxicity. Kozuru, M., Uike, N., Takeichi, N., Nakagawa, S., Takahira, H., Shibata, K., Sato, H. Br. J. Haematol. (1989) [Pubmed]
  15. The effect of prostaglandin and its inhibitor on antibody-dependent cellular cytotoxicity against human squamous cell carcinoma of head and neck. Kim, C.D., Sung, M.W., Lee, S.J., Heo, D.S., Yoon, S.J., Kim, K.H. Anticancer Res. (1999) [Pubmed]
  16. Autosomal dominant congenital cataract associated with a missense mutation in the human alpha crystallin gene CRYAA. Litt, M., Kramer, P., LaMorticella, D.M., Murphey, W., Lovrien, E.W., Weleber, R.G. Hum. Mol. Genet. (1998) [Pubmed]
  17. Cytokine influence on killing of fresh chronic lymphocytic leukemia cells by human leukocytes. Cemerlíc, D., Dadey, B., Han, T., Vaickus, L. Blood (1991) [Pubmed]
  18. Modulation of human polymorphonuclear leukocyte IgG Fc receptors and Fc receptor-mediated functions by IFN-gamma and glucocorticoids. Petroni, K.C., Shen, L., Guyre, P.M. J. Immunol. (1988) [Pubmed]
  19. The genetic deficiency of leukocyte surface glycoprotein Mac-1, LFA-1, p150,95 in humans is associated with defective antibody-dependent cellular cytotoxicity in vitro and defective protection against herpes simplex virus infection in vivo. Kohl, S., Loo, L.S., Schmalstieg, F.S., Anderson, D.C. J. Immunol. (1986) [Pubmed]
  20. Importance of immunoglobulin isotype in human antibody-dependent, cell-mediated cytotoxicity directed by murine monoclonal antibodies. Kipps, T.J., Parham, P., Punt, J., Herzenberg, L.A. J. Exp. Med. (1985) [Pubmed]
  21. Human antibody-dependent cellular cytotoxicity. Isolation and identification of a subpopulation of peripheral blood lymphocytes which kill antibody-coated autologous target cells. Brier, A.M., Chess, L., Schlossman, S.F. J. Clin. Invest. (1975) [Pubmed]
  22. Antibodies to T cells in patients with systemic lupus erythematosus can induce antibody-dependent cell-mediated cytotoxicity against human T cells. Kumagai, S., Steinberg, A.D., Green, I. J. Clin. Invest. (1981) [Pubmed]
  23. Biological activity of human-mouse IgG1, IgG2, IgG3, and IgG4 chimeric monoclonal antibodies with antitumor specificity. Steplewski, Z., Sun, L.K., Shearman, C.W., Ghrayeb, J., Daddona, P., Koprowski, H. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  24. Interaction of target cell-bound C3bi and C3d with human lymphocyte receptors. Enhancement of antibody-mediated cellular cytotoxicity. Perlmann, H., Perlmann, P., Schreiber, R.D., Müller-Eberhard, H.J. J. Exp. Med. (1981) [Pubmed]
  25. Immunologic control of a retrovirus-associated murine adenocarcinoma. VIII. Corynebacterium parvum-activated natural killer cells as potent antibody-dependent cell-mediated cytotoxicity effectors. Weinhold, K.J., Bolognesi, D.P., Matthews, T.J. J. Natl. Cancer Inst. (1985) [Pubmed]
  26. Correlation between natural and antibody-dependent cell-mediated cytotoxicity against tumor targets in the mouse. II. Characterization of the effector cells. Santoni, A., Herberman, R.B., Holden, H.T. J. Natl. Cancer Inst. (1979) [Pubmed]
  27. Monoclonal Lym-1 antibody-dependent lysis of B-lymphoblastoid tumor targets by human complement and cytokinine-exposed mononuclear and neutrophilic polymorphonuclear leukocytes. Ottonello, L., Morone, P., Dapino, P., Dallegri, F. Blood (1996) [Pubmed]
  28. Involvement of the high-affinity receptor for IgG (Fc gamma RI; CD64) in enhanced tumor cell cytotoxicity of neutrophils during granulocyte colony-stimulating factor therapy. Valerius, T., Repp, R., de Wit, T.P., Berthold, S., Platzer, E., Kalden, J.R., Gramatzki, M., van de Winkel, J.G. Blood (1993) [Pubmed]
  29. Expression of rat complement control protein Crry on tumor cells inhibits rat natural killer cell-mediated cytotoxicity. Caragine, T.A., Imai, M., Frey, A.B., Tomlinson, S. Blood (2002) [Pubmed]
  30. Chimeric CD19 antibody mediates cytotoxic activity against leukemic blasts with effector cells from pediatric patients who received T-cell-depleted allografts. Lang, P., Barbin, K., Feuchtinger, T., Greil, J., Peipp, M., Zunino, S.J., Pfeiffer, M., Handgretinger, R., Niethammer, D., Fey, G.H. Blood (2004) [Pubmed]
  31. Autosomal dominant cerulean cataract is associated with a chain termination mutation in the human beta-crystallin gene CRYBB2. Litt, M., Carrero-Valenzuela, R., LaMorticella, D.M., Schultz, D.W., Mitchell, T.N., Kramer, P., Maumenee, I.H. Hum. Mol. Genet. (1997) [Pubmed]
  32. What is the best predictor of the severity of ABO-haemolytic disease of the newborn? Brouwers, H.A., Overbeeke, M.A., van Ertbruggen, I., Schaasberg, W., Alsbach, G.P., van der Heiden, C., van Leeuwen, E.F., Stoop, J.W., Engelfriet, C.P. Lancet (1988) [Pubmed]
  33. An anti-CD20-IL-2 immunocytokine is highly efficacious in a SCID mouse model of established human B lymphoma. Gillies, S.D., Lan, Y., Williams, S., Carr, F., Forman, S., Raubitschek, A., Lo, K.M. Blood (2005) [Pubmed]
  34. Humanized anti-HM1.24 antibody mediates myeloma cell cytotoxicity that is enhanced by cytokine stimulation of effector cells. Ozaki, S., Kosaka, M., Wakahara, Y., Ozaki, Y., Tsuchiya, M., Koishihara, Y., Goto, T., Matsumoto, T. Blood (1999) [Pubmed]
  35. The effect of various cytokines on the in vitro induction of antibody-dependent cellular cytotoxicity in murine cells. Enhancement of IL-2-induced antibody-dependent cellular cytotoxicity activity by IL-1 and tumor necrosis factor-alpha. Eisenthal, A., Rosenberg, S.A. J. Immunol. (1989) [Pubmed]
  36. Functional heterogeneity between NKR-P1bright/Lycopersicon esculentum lectin (L.E.)bright and NKR-P1bright/L.E.dim subpopulations of rat natural killer cells. Chambers, W.H., Brumfield, A.M., Hanley-Yanez, K., Lakomy, R., Herberman, R.B., McCaslin, D.C., Olszowy, M.W., McCoy, J.P. J. Immunol. (1992) [Pubmed]
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