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Gene Review

USP5  -  ubiquitin specific peptidase 5...

Homo sapiens

Synonyms: Deubiquitinating enzyme 5, ISOT, IsoT, Isopeptidase T, Ubiquitin carboxyl-terminal hydrolase 5, ...
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Disease relevance of USP5


High impact information on USP5

  • Unlike other ubiquitin binding domains, this domain contains a deep binding pocket where the C-terminal diglycine motif of ubiquitin is inserted, thus explaining the specificity of IsoT for an unmodified C terminus on the proximal subunit of polyubiquitin [2].
  • The activity of specific USPs, including USP5, -7, -9, -13, -15, and -22, was up-regulated by mitogen activation or virus infection in normal T and B lymphocytes [3].
  • To determine whether specificity may be conferred by recognition of a primary cognate sequence, the substrate preferences of two DUBs, UCH-L3 and isopeptidase T (IsoT), were profiled using a positional scanning branched peptide library [4].
  • The inhibitor selectivity for deconjugation of the 26 and 17 kDa conjugates was similar to that observed for isopeptidase T. The observations suggest that these two conjugated proteins of the reticulocyte lysate are specific substrates for isopeptidase T in lysates [5].
  • To explain these observations, we propose a structural model for isopeptidase T that involves two binding sites for ubiquitin [6].

Biological context of USP5


Anatomical context of USP5

  • Isopeptidase T was shown to specifically disassemble two conjugates (assumed to be di- and triubiquitin with masses of 26 and 17 kDa) formed in the reticulocyte lysate system [5].

Associations of USP5 with chemical compounds

  • Results from an earlier ENDOR study on X-irradiated sucrose single crystals (Vanhaelewyn et al., Appl Radiat Isot, 52, 1221 (2000)) were used for analyzing of the spectra in more details [9].
  • A method is described for the quantitative analysis of isot retinoin and its 4-oxo metabolite, or of etretinate and its principal metabolites, in human blood in the range 10-2000 ng/ml [10].
  • The most differentially abundant proteins between cancer and benign samples were isopeptidase T, serum amyloid P (SAP), annexin A3 (ANXA3) and mitochondrial enoyl coenzyme-A hydratase [11].

Physical interactions of USP5


Other interactions of USP5


Analytical, diagnostic and therapeutic context of USP5

  • In the present study, 44 red-scaled pepper and 26 red powder pepper samples obtained from various spice retailers in Erzurum, and 20 isot (red pepper produced in Sanliurfa) were investigated for aflatoxin contamination using thin layer chromatography [12].


  1. Selective upregulation of the ubiquitin-proteasome proteolytic pathway proteins, proteasome zeta chain and isopeptidase T in fetal Down syndrome. Engidawork, E., Juranville, J.F., Fountoulakis, M., Dierssen, M., Lubec, G. J. Neural Transm. Suppl. (2001) [Pubmed]
  2. The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitin. Reyes-Turcu, F.E., Horton, J.R., Mullally, J.E., Heroux, A., Cheng, X., Wilkinson, K.D. Cell (2006) [Pubmed]
  3. Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells. Ovaa, H., Kessler, B.M., Rolén, U., Galardy, P.J., Ploegh, H.L., Masucci, M.G. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  4. Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry. Mason, D.E., Ek, J., Peters, E.C., Harris, J.L. Biochemistry (2004) [Pubmed]
  5. Nonhydrolyzable diubiquitin analogues are inhibitors of ubiquitin conjugation and deconjugation. Yin, L., Krantz, B., Russell, N.S., Deshpande, S., Wilkinson, K.D. Biochemistry (2000) [Pubmed]
  6. Kinetic studies of isopeptidase T: modulation of peptidase activity by ubiquitin. Stein, R.L., Chen, Z., Melandri, F. Biochemistry (1995) [Pubmed]
  7. Further characterization of the putative human isopeptidase T catalytic site. Lacombe, T., Gabriel, J.M. FEBS Lett. (2002) [Pubmed]
  8. Osmoregulatory control of renal sodium excretion after sodium loading in humans. Andersen, L.J., Norsk, P., Johansen, L.B., Christensen, P., Engstrom, T., Bie, P. Am. J. Physiol. (1998) [Pubmed]
  9. High-field/ high-frequency EPR study on stable free radicals formed in sucrose by gamma-irradiation. Georgieva, E.R., Pardi, L., Jeschke, G., Gatteschi, D., Sorace, L., Yordanov, N.D. Free Radic. Res. (2006) [Pubmed]
  10. Determination of isotretinoin or etretinate and their major metabolites in human blood by reversed-phase high-performance liquid chromatography. Buggé, C.J., Rodriguez, L.C., Vane, F.M. Journal of pharmaceutical and biomedical analysis. (1985) [Pubmed]
  11. Differential radioactive quantification of protein abundance ratios between benign and malignant prostate tissues: Cancer association of annexin A3. Wozny, W., Schroer, K., Schwall, G.P., Poznanovi??, S., Stegmann, W., Dietz, K., Rogatsch, H., Schaefer, G., Huebl, H., Klocker, H., Schrattenholz, A., Cahill, M.A. Proteomics (2007) [Pubmed]
  12. The aflatoxin contamination of some pepper types sold in Turkey. Erdogan, A. Chemosphere (2004) [Pubmed]
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