Disease relevance of ZBP1

• Z alpha domains have been found previously in two other IFN-inducible proteins, the dsRNA editing enzyme, ADAR1, and Z-DNA binding protein 1 (ZBP1), as well as in the poxvirus virulence factor, E3L [1].
• ZBP1 was distributed primarily in the cytoplasm and occasionally as nuclear foci in interferon (IFN)-treated primary hepatocellular carcinoma cells, and in several other transfected cell types [2].
• We here report the cloning by differential display of a novel gene, DLM-1, that is highly up-regulated in the peritoneal lining tissue of mice bearing MOT ascites tumors [3].

Psychiatry related information on ZBP1

High impact information on ZBP1

• We find that human DLM-1 is predominantly expressed in lymphatic tissues [4].
• Cloning of DLM-1, a novel gene that is up-regulated in activated macrophages, using RNA differential display [3].
• DLM-1 RNA was detected at significant levels in normal mouse lung, intestinal epithelium, liver and thymus by Northern blot analysis [3].

Associations of ZBP1 with chemical compounds

• In leptomycin B (LMB)-treated cells, endogenous ZBP1 efficiently accumulated in nuclear foci, which overlapped PML oncogenic domains (PODs) or nuclear bodies (NBs) [2] .
• Heat shock and arsenite exposure had opposing effects on ZBP1 isoforms: while ZBP1DeltaZalpha granules disassembled, full length ZBP1 accumulated in SGs [5].
• Up-regulation of DLM-1 became evident by 4h after stimulation with IFN-gamma and was not blocked by cycloheximide, suggesting the presence of IFN responding elements in its transcription regulation region [3].
• A Z-DNA binding protein of 140,000 M(r) has been purified from chicken lungs by sedimentation through 40%(w/w) sucrose and Z-DNA affinity chromatography [6].

Physical interactions of ZBP1

• DLM-1 contains an N-terminal Z-DNA binding domain homologous to the Zalpha domain in the RNA editing enzyme ADAR1 [4].

Other interactions of ZBP1

• Mouse peritoneal macrophages, stimulated by IFN-gamma or LPS, also expressed significant amounts of DLM-1 [3].
• Experiments were carried out with a Z-DNA-binding protein domain from the editing enzyme, double-stranded RNA adenosine deaminase 1 [7].

Analytical, diagnostic and therapeutic context of ZBP1

• Here, we attempted to characterize the Z-DNA binding activities of two Zalpha domains in the human ZBP1, hZalpha(ZBP1) and hZbeta(ZBP1), using circular dichroism (CD) [8].

References