Disease relevance of DND1

• The Arabidopsis thaliana dnd1 mutant was previously isolated as a line that failed to produce the HR in response to avirulent Pseudomonas syringae pathogens; plants homozygous for the recessive dnd1-1 mutation still carry out effective gene-for-gene resistance [1].

High impact information on DND1

• DND1 encodes the same protein as AtCNGC2, a cyclic nucleotide-gated ion channel of previously unknown organismal function that can allow passage of Ca(2+), K(+) and other cations [Leng, Q., Mercier, R. W., Yao, W. & Berkowitz, G. A. (1999) Plant Physiol. 121, 753-761] [1].
• Plants mutated at the DND1 locus are defective in HR cell death but retain characteristic responses to avirulent Pseudomonas syringae such as induction of pathogenesis-related gene expression and strong restriction of pathogen growth [2].
• Gene-for-gene disease resistance without the hypersensitive response in Arabidopsis dnd1 mutant [2].
• Levels of salicylic acid compounds and mRNAs for pathogenesis-related genes are elevated constitutively in dnd1 plants [2].
• Several other genes with regulatory functions have been identified by mutation, including DND1, PAD4, CPR6, and SSl1 [3].

Biological context of DND1

• A previous mutant screen identified Arabidopsis dnd1 and dnd2 "defense, no death" mutants, which exhibit loss of hypersensitive response (HR) cell death without loss of gene-for-gene resistance [4].
• The dnd1 phenotype is caused by mutation of the gene encoding cyclic nucleotide-gated (CNG) ion channel AtCNGC2 [4].
• AtCNGC2 was demonstrated to conduct K(+) and other monovalent cations, but exclude Na(+); this conductivity profile is unique for any ion channel not possessing the amino acid sequence found in the selectivity filter of K(+)-selective ion channels [5].
• Investigations using the promoter::GUS plants demonstrated that AtCNGC2 is expressed in flowers during organ senescence and in the dehiscence zone of siliques [6].

Anatomical context of DND1

• Application of cAMP evoked currents in membrane patches of oocytes injected with AtCNGC2 cRNA [5].

Associations of DND1 with chemical compounds

• Expression of AtCNGC2 and AtCNGC4 enhanced growth of trk1,2 in the presence of hygromycin; AtCNGC1 has less of an effect [7].

Other interactions of DND1

• The Arabidopsis dnd1 "defense, no death" gene encodes a mutated cyclic nucleotide-gated ion channel [1].

Analytical, diagnostic and therapeutic context of DND1

• Furthermore, expression of AtCNGC2 was transiently induced during leaf and cell culture senescence [6].

References