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CAB2  -  chlorophyll A/B-binding protein 2

Arabidopsis thaliana

Synonyms: AB165, F1N18.4, F1N18_4, LHCB1.1, LIGHT HARVESTING CHLOROPHYLL A/B-BINDING PROTEIN 1.1
 
 
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Disease relevance of CAB2

 

High impact information on CAB2

  • Plants overexpressing ELF3 have an increased period length in both constant blue and red light; furthermore, etiolated ELF3-overexpressing seedlings exhibit a decreased acute CAB2 response after a red light pulse, whereas the null mutant is hypersensitive to acute induction [2].
  • Based on these genetic studies, we propose a model for the regulation of CAB2 transcription in which individual photoreceptors and phototransduction components have been assigned to specific pathways for the regulation of discrete kinetic components of the CAB2 expression pattern [3].
  • We have used a fusion of the promoter of a chlorophyll a/b binding protein gene, CAB2, with firefly luciferase (cab2::luc) to monitor the detailed kinetics of transcription in response to photoreceptor activation in Arabidopsis [3].
  • The tobacco nuclear proteins CUF-1 (CAB upstream factor 1) and CGF-1 (CAB GATA factor 1) bind the Arabidopsis CAB2 promoter, and the CGF-1 binding site is contained within a minimal clock- and phytochrome-regulated region of the promoter [4].
  • This is based both on morphological phenotypes, such as hypocotyl elongation and hook and cotyledon opening, and on molecular phenotypes, such as misregulation of the light-controlled genes CAB2 and RbcS [5].
 

Biological context of CAB2

  • Using this marker in phototransduction and circadian-dysfunctional mutants, we studied how signals from phytochrome and the circadian clock are integrated for the regulation of CAB2 transcription [3].
  • We have used in vivo cab2::luciferase gene bioluminescence markers containing site-directed mutations in the CUF-1 and CGF-1 binding sites to define the role of these proteins in CAB2 regulation and to further delineate the terminal genomic targets of the phytochrome and circadian clock signal transduction pathways [4].
  • Regulation of Cat3 gene expression is not dependent on the presence of the Tourist element in the promoter of the gene nor on the presence of motifs similar to those found significant in the circadian expression of the Arabidopsis CAB2 gene [6].
  • These data indicate that both oscillators regulate the expression of the Cab genes studied at the level of transcription and that the cis-acting element(s) of the wheat Cab-1 and A. thaliana Cab2 genes mediating these responses are located on short, 250 bp promoter regions [7].
  • The study involved analysing the expression patterns of transgenes, containing short fragments of the Arabidopsis thaliana Cab2 or the wheat Cab-1 promoter fused to the firefly luciferase reporter gene, by a video-imaging system in single, etiolated tobacco seedlings [7].
 

Other interactions of CAB2

  • Additionally, the mRNA from one of the three known A. thaliana cab genes, AB140, is similar in quantity to the mRNAs from the other two, AB165 and AB180, in dark-grown seedlings of hy-3 and hy-5 as well as the parent A. thaliana (Landsberg) after a brief red light treatment [8].
  • The free-running robust rhythm of CAB2 was also dampened in APRR1-ox [9].
  • A 40-bp DET1 dark-response element (DtRE) is required for both dark and root-specific repression of CAB2, whereas the known CAB upstream factor-1 element is required for DET1 activation-associated effects in the light and repression in the roots [10].
  • DtRE binds two distinct activities in Arabidopsis seedling extracts: a novel activity with binding site CAAAACGC that we have named CAB2 DET1-associated factor 1 plus an activity that is likely to be the myb transcription factor Circadian Clock-Associated 1 [10].
 

Analytical, diagnostic and therapeutic context of CAB2

References

  1. Molecular dissection of GT-1 from Arabidopsis. Hiratsuka, K., Wu, X., Fukuzawa, H., Chua, N.H. Plant Cell (1994) [Pubmed]
  2. ELF3 modulates resetting of the circadian clock in Arabidopsis. Covington, M.F., Panda, S., Liu, X.L., Strayer, C.A., Wagner, D.R., Kay, S.A. Plant Cell (2001) [Pubmed]
  3. Attenuation of phytochrome A and B signaling pathways by the Arabidopsis circadian clock. Anderson, S.L., Somers, D.E., Millar, A.J., Hanson, K., Chory, J., Kay, S.A. Plant Cell (1997) [Pubmed]
  4. Functional dissection of circadian clock- and phytochrome-regulated transcription of the Arabidopsis CAB2 gene. Anderson, S.L., Kay, S.A. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  5. Gibberellins repress photomorphogenesis in darkness. Alabadí, D., Gil, J., Blázquez, M.A., García-Martínez, J.L. Plant Physiol. (2004) [Pubmed]
  6. Circadian expression of the maize catalase Cat3 gene is highly conserved among diverse maize genotypes with structurally different promoters. Polidoros, A.N., Scandalios, J.G. Genetics (1998) [Pubmed]
  7. Transcription of Arabidopsis and wheat Cab genes in single tobacco transgenic seedlings exhibits independent rhythms in a developmentally regulated fashion. Kolar, C., Fejes, E., Adám, E., Schäfer, E., Kay, S., Nagy, F. Plant J. (1998) [Pubmed]
  8. Phytochrome-regulated expression of genes encoding light-harvesting chlorophyll a/b-protein in two long hypocotyl mutants and wild type plants of Arabidopsis thaliana. Sun, L., Tobin, E.M. Photochem. Photobiol. (1990) [Pubmed]
  9. The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana: I. Characterization with APRR1-overexpressing plants. Makino, S., Matsushika, A., Kojima, M., Yamashino, T., Mizuno, T. Plant Cell Physiol. (2002) [Pubmed]
  10. HY5, Circadian Clock-Associated 1, and a cis-element, DET1 dark response element, mediate DET1 regulation of chlorophyll a/b-binding protein 2 expression. Maxwell, B.B., Andersson, C.R., Poole, D.S., Kay, S.A., Chory, J. Plant Physiol. (2003) [Pubmed]
 
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