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Gene Review

PTR2  -  Ptr2p

Saccharomyces cerevisiae S288c

Synonyms: Peptide permease PTR2, Peptide transporter PTR2, YKR093W, YKR413
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Disease relevance of PTR2


High impact information on PTR2

  • Cup9p acts as a repressor of PTR2, a gene encoding the transmembrane peptide transporter [2].
  • The Ubr1p-Cup9p-Ptr2p circuit is the first example of a physiological process controlled by the N-end rule pathway [2].
  • PTR2 was tightly linked to the UBI2 gene, with the coding sequences being separated by a 466-bp region and oriented so that the genes were transcribed convergently [1].
  • A chromosomal disruption of the PTR2 gene in a haploid strain was not lethal under standard growth conditions [1].
  • We have cloned and characterized a Saccharomyces cerevisiae peptide transport gene (PTR2) isolated from a genomic DNA library by directly selecting for functional complementation of a peptide transport-deficient mutant [1].

Biological context of PTR2

  • The cloning of PTR2 represents the first example of the molecular genetic characterization of a eucaryotic peptide transport gene [1].
  • To test the role of TM5 in Ptr2p function, Ala-scanning mutagenesis of the 22 residues comprising TM5 was completed [3].
  • The deletion analysis of the PTR 2 promoter region confirmed these suggestions and revealed that the cis-element involved in the regulation of the PTR 2 gene by amino acids is located in the region from residue --400 to the start codon [4].

Associations of PTR2 with chemical compounds

  • Moreover, even in the absence of Dal5p and Ptr2p, an additional activity--almost certainly the periplasmic asparaginase II Asp3p--facilitates the utilization of dipeptides with C-terminal asparagine residues by a different strategy [5].
  • Northern blot analysis suggested that asparagine and serine repress the expression of the PTR 2 gene, but lysine decreases the peptide transport activity without repressing PTR 2 gene transcription [4].

Regulatory relationships of PTR2

  • PTR3 was required for amino acid-induced expression of PTR2, the gene encoding the dipeptide/tripeptide transport protein, but was not necessary for nitrogen catabolite repression of peptide import or PTR2 expression [6].

Other interactions of PTR2

  • Further, ptr1 mutants did not express PTR2, a gene encoding the integral membrane component required for peptide transport in S. cerevisiae [7].
  • In response to external amino acids, the permease-like sensor Ssy1 of Saccharomyces cerevisiae activates a pathway leading to transcriptional induction of several permease genes including AGP1 and PTR2 [8].
  • Isolation and screening of 31 additional N-methyl-N-nitro-N-Nitrosoguanidine (MNNG)-induced, peptide transport-deficient mutants produced one ptr3 and 30 ptr2 strains: no additional complementation groups were detected [9].


  1. Isolation and characterization of a Saccharomyces cerevisiae peptide transport gene. Perry, J.R., Basrai, M.A., Steiner, H.Y., Naider, F., Becker, J.M. Mol. Cell. Biol. (1994) [Pubmed]
  2. The N-end rule pathway controls the import of peptides through degradation of a transcriptional repressor. Byrd, C., Turner, G.C., Varshavsky, A. EMBO J. (1998) [Pubmed]
  3. Substrate preference is altered by mutations in the fifth transmembrane domain of Ptr2p, the di/tri-peptide transporter of Saccharomyces cerevisiae. Hauser, M., Kauffman, S., Naider, F., Becker, J.M. Mol. Membr. Biol. (2005) [Pubmed]
  4. Effect of amino acids on peptide transport in sake yeast. Yamada, T., Furukawa, K., Hara, S., Mizoguchi, H. J. Biosci. Bioeng. (2005) [Pubmed]
  5. Harnessing natural diversity to probe metabolic pathways. Homann, O.R., Cai, H., Becker, J.M., Lindquist, S.L. PLoS Genet. (2005) [Pubmed]
  6. PTR3, a novel gene mediating amino acid-inducible regulation of peptide transport in Saccharomyces cerevisiae. Barnes, D., Lai, W., Breslav, M., Naider, F., Becker, J.M. Mol. Microbiol. (1998) [Pubmed]
  7. A recognition component of the ubiquitin system is required for peptide transport in Saccharomyces cerevisiae. Alagramam, K., Naider, F., Becker, J.M. Mol. Microbiol. (1995) [Pubmed]
  8. Ubiquitin and the SCF(Grr1) ubiquitin ligase complex are involved in the signalling pathway activated by external amino acids in Saccharomyces cerevisiae. Bernard, F., André, B. FEBS Lett. (2001) [Pubmed]
  9. Isolation and characterization of S. cerevisiae mutants deficient in amino acid-inducible peptide transport. Island, M.D., Perry, J.R., Naider, F., Becker, J.M. Curr. Genet. (1991) [Pubmed]
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