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Gene Review

SST2  -  Sst2p

Saccharomyces cerevisiae S288c

Synonyms: L9324.9, Protein SST2, YLR452C
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Disease relevance of SST2


High impact information on SST2

  • G beta phosphorylation does not require either the pheromone receptor C-terminus or the product of the SST2 gene, both of which mediate separate adaptive responses to pheromone [2].
  • Because SST2 product and the receptor tail contribute independently to events that allow recovery from pheromone-induced growth arrest, KSS1 function defines a third independent process that promotes desensitization [3].
  • We conclude that the C-terminus of the receptor is responsible for one aspect of cellular adaptation to pheromone that is distinct from adaptation controlled by the SST2 gene, for decreasing the stability of the receptor, and for some aspect of cellular morphogenesis [4].
  • Significant homology was also found to the SST2 gene product in Saccharomyces cerevisiae that is known to interact with a yeast G alpha subunit (Gpa1) [5].
  • Although the SST2 mutations confer a pheromone-resistant phenotype, they do not prevent downstream activation by overexpression of G beta (STE4), a constitutively active G beta mutation (STE4Hpl), or a disruption of GPA1 [6].

Biological context of SST2

  • HMG1 was mapped to the left arm of chromosome XIII near SUP79, and HMG2 was mapped to the right arm of chromosome XII near SST2 [7].
  • Yeast strains with a disrupted SST2 gene, which is a member of the RGS (regulator of G protein signaling) family, and a disrupted FAR1 gene, which mediates cell cycle arrest in response to a pheromone, were monitored by measuring their fluorescence and growth rate [8].
  • This phenotype suggests that SST2 encodes a component of the pheromone response pathway that is common to both mating types [9].
  • We have cloned the SST2 gene by isolation of multicopy plasmids that complement the sst2-1 mutation [9].
  • The regulator of G-protein signaling (RGS) proteins have recently been identified as signal transduction molecules which have structural homology to SST2 of Saccharomyces cerevisiae and EGL-10 of Caenorhabditis elegans [10].

Regulatory relationships of SST2

  • The Saccharomyces cerevisiae RGS protein Sst2p is involved in desensitization to pheromone and acts as a GTPase-activating protein for the Galpha subunit Gpa1p [11].

Other interactions of SST2

  • We identified two genes, SST2 and ASG7 [12].
  • Forty-eight mRNAs were found to change significantly in translation state following release from alpha-factor arrest, including genes involved in pheromone response and cell cycle arrest such as BAR1, SST2, and FAR1 [13].
  • Autocrine activation of the pheromone response pathway in matalpha2- cells is attenuated by SST2- and ASG7-dependent mechanisms [12].

Analytical, diagnostic and therapeutic context of SST2

  • Sequence analysis of a nearly full-length flbA cDNA clone showed that flbA is predicted to encode a 717-amino-acid polypeptide with 30% identity to the Saccharomyces cerevisiae SST2 protein [14].


  1. An RGS protein regulates the pheromone response in the fission yeast Schizosaccharomyces pombe. Watson, P., Davis, K., Didmon, M., Broad, P., Davey, J. Mol. Microbiol. (1999) [Pubmed]
  2. Pheromone-induced phosphorylation of a G protein beta subunit in S. cerevisiae is associated with an adaptive response to mating pheromone. Cole, G.M., Reed, S.I. Cell (1991) [Pubmed]
  3. A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae. Courchesne, W.E., Kunisawa, R., Thorner, J. Cell (1989) [Pubmed]
  4. The C-terminus of the S. cerevisiae alpha-pheromone receptor mediates an adaptive response to pheromone. Konopka, J.B., Jenness, D.D., Hartwell, L.H. Cell (1988) [Pubmed]
  5. GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain. De Vries, L., Mousli, M., Wurmser, A., Farquhar, M.G. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  6. Inhibition of G-protein signaling by dominant gain-of-function mutations in Sst2p, a pheromone desensitization factor in Saccharomyces cerevisiae. Dohlman, H.G., Apaniesk, D., Chen, Y., Song, J., Nusskern, D. Mol. Cell. Biol. (1995) [Pubmed]
  7. Identifying mutations in duplicated functions in Saccharomyces cerevisiae: recessive mutations in HMG-CoA reductase genes. Basson, M.E., Moore, R.L., O'Rear, J., Rine, J. Genetics (1987) [Pubmed]
  8. Quantitative and dynamic analyses of G protein-coupled receptor signaling in yeast using Fus1, enhanced green fluorescence protein (EGFP), and His3 fusion protein. Ishii, J., Matsumura, S., Kimura, S., Tatematsu, K., Kuroda, S., Fukuda, H., Kondo, A. Biotechnol. Prog. (2006) [Pubmed]
  9. Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone. Dietzel, C., Kurjan, J. Mol. Cell. Biol. (1987) [Pubmed]
  10. Isolation, tissue expression, and chromosomal assignment of human RGS5, a novel G-protein signaling regulator gene. Seki, N., Sugano, S., Suzuki, Y., Nakagawara, A., Ohira, M., Muramatsu, M., Saito, T., Hori, T. J. Hum. Genet. (1998) [Pubmed]
  11. The N terminus of Saccharomyces cerevisiae Sst2p plays an RGS-domain-independent, Mpt5p-dependent role in recovery from pheromone arrest. Xu, B.E., Skowronek, K.R., Kurjan, J. Genetics (2001) [Pubmed]
  12. Autocrine activation of the pheromone response pathway in matalpha2- cells is attenuated by SST2- and ASG7-dependent mechanisms. Rivers, D.M., Sprague, G.F. Mol. Genet. Genomics (2003) [Pubmed]
  13. The transcriptome and its translation during recovery from cell cycle arrest in Saccharomyces cerevisiae. Serikawa, K.A., Xu, X.L., MacKay, V.L., Law, G.L., Zong, Q., Zhao, L.P., Bumgarner, R., Morris, D.R. Mol. Cell Proteomics (2003) [Pubmed]
  14. Overexpression of flbA, an early regulator of Aspergillus asexual sporulation, leads to activation of brlA and premature initiation of development. Lee, B.N., Adams, T.H. Mol. Microbiol. (1994) [Pubmed]
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