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Gene Review

CDC53  -  cullin CDC53

Saccharomyces cerevisiae S288c

Synonyms: Cell division control protein 53, Cullin-A, D2190, E3 ubiquitin ligase complex SCF subunit CDC53, YDL132W
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High impact information on CDC53

  • Cdc53 targets phosphorylated G1 cyclins for degradation by the ubiquitin proteolytic pathway [1].
  • We conclude that Cdc53/Hrt1 comprise a highly conserved module that serves as the functional core of a broad variety of heteromeric ubiquitin ligases [2].
  • SCFCdc4 (Skp1, Cdc53/cullin, F-box protein) defines a family of modular ubiquitin ligases (E3s) that regulate diverse processes including cell cycle, immune response, and development [2].
  • Two genes have been identified that are required for Rub1p conjugation to Cdc53p [3].
  • In contrast, the Cdc34-Cdc53-Skp1 E2/E3 core complex is required for all three functions [4].

Biological context of CDC53

  • Mutations in CDC53 cause a phenotype indistinguishable from those of cdc4 and cdc34 mutations, numerous genetic interactions are seen between these genes, and the encoded proteins are found physically associated in vivo [5].
  • These observations suggest that Mfb1p acts in a complex lacking Cdc53p required for mitochondrial morphogenesis [6].
  • The 44-bp palindrome found between the genes CDC53 and LYS21 on chromosome IV was the longest palindrome identified and contained only two C-G base pairs [7].
  • Defective chromatin assembly extracts generated from strains harboring a mutation in the Cdc53p SCF subunit or a nondegradable SCF target, Sic1(Deltaphos), confirmed that the SCF was involved in mitotic chromatin assembly [8].
  • Both in vivo and in vitro experiments indicate that Dcn1p does not inhibit deneddylation of Cdc53p by the COP9 signalosome, but greatly increases the kinetics of the neddylation reaction [9].

Other interactions of CDC53

  • An essential domain within Cdc34p is required for binding to a complex containing Cdc4p and Cdc53p in Saccharomyces cerevisiae [10].
  • Through mass spectrometric analysis of Cdc53 associated proteins, we have identified three novel F-box proteins that appear to participate in SCF-like complexes [11].
  • Sgt1p is a highly conserved eucaryotic protein that is required for both SCF (Skp1p/Cdc53p-Cullin-F-box)-mediated ubiquitination and kinetochore function in yeast [12].
  • Previous systematic genome-wide two-hybrid screens yielded 17 proteins that interact with Skp1 and Cdc53, only 3 of which overlap with those reported here [13].
  • A newly discovered family of proteins homologous to yeast Cdc53, called cullins, may play a key role in the targeting of cell-cycle regulators, such as cyclins, for destruction by ubiquitin-dependent proteolysis [14].


  1. Cdc53 targets phosphorylated G1 cyclins for degradation by the ubiquitin proteolytic pathway. Willems, A.R., Lanker, S., Patton, E.E., Craig, K.L., Nason, T.F., Mathias, N., Kobayashi, R., Wittenberg, C., Tyers, M. Cell (1996) [Pubmed]
  2. Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Seol, J.H., Feldman, R.M., Zachariae, W., Shevchenko, A., Correll, C.C., Lyapina, S., Chi, Y., Galova, M., Claypool, J., Sandmeyer, S., Nasmyth, K., Deshaies, R.J., Shevchenko, A., Deshaies, R.J. Genes Dev. (1999) [Pubmed]
  3. Modification of yeast Cdc53p by the ubiquitin-related protein rub1p affects function of the SCFCdc4 complex. Lammer, D., Mathias, N., Laplaza, J.M., Jiang, W., Liu, Y., Callis, J., Goebl, M., Estelle, M. Genes Dev. (1998) [Pubmed]
  4. Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box proteincomplexes that regulate cell division and methionine biosynthesis in yeast. Patton, E.E., Willems, A.R., Sa, D., Kuras, L., Thomas, D., Craig, K.L., Tyers, M. Genes Dev. (1998) [Pubmed]
  5. Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins. Mathias, N., Johnson, S.L., Winey, M., Adams, A.E., Goetsch, L., Pringle, J.R., Byers, B., Goebl, M.G. Mol. Cell. Biol. (1996) [Pubmed]
  6. The novel F-box protein Mfb1p regulates mitochondrial connectivity and exhibits asymmetric localization in yeast. Kondo-Okamoto, N., Ohkuni, K., Kitagawa, K., McCaffery, J.M., Shaw, J.M., Okamoto, K. Mol. Biol. Cell (2006) [Pubmed]
  7. Palindrome content of the yeast Saccharomyces cerevisiae genome. Lisnić, B., Svetec, I.K., Sarić, H., Nikolić, I., Zgaga, Z. Curr. Genet. (2005) [Pubmed]
  8. Novel interaction between Apc5p and Rsp5p in an intracellular signaling pathway in Saccharomyces cerevisiae. Arnason, T.G., Pisclevich, M.G., Dash, M.D., Davies, G.F., Harkness, T.A. Eukaryotic Cell (2005) [Pubmed]
  9. The conserved protein DCN-1/Dcn1p is required for cullin neddylation in C. elegans and S. cerevisiae. Kurz, T., Ozlü, N., Rudolf, F., O'Rourke, S.M., Luke, B., Hofmann, K., Hyman, A.A., Bowerman, B., Peter, M. Nature (2005) [Pubmed]
  10. An essential domain within Cdc34p is required for binding to a complex containing Cdc4p and Cdc53p in Saccharomyces cerevisiae. Mathias, N., Steussy, C.N., Goebl, M.G. J. Biol. Chem. (1998) [Pubmed]
  11. SCF ubiquitin protein ligases and phosphorylation-dependent proteolysis. Willems, A.R., Goh, T., Taylor, L., Chernushevich, I., Shevchenko, A., Tyers, M. Philos. Trans. R. Soc. Lond., B, Biol. Sci. (1999) [Pubmed]
  12. Sgt1p contributes to cyclic AMP pathway activity and physically interacts with the adenylyl cyclase Cyr1p/Cdc35p in budding yeast. Dubacq, C., Guerois, R., Courbeyrette, R., Kitagawa, K., Mann, C. Eukaryotic Cell (2002) [Pubmed]
  13. Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Seol, J.H., Shevchenko, A., Shevchenko, A., Deshaies, R.J. Nat. Cell Biol. (2001) [Pubmed]
  14. Cell cycle: cull and destroy. Jackson, P.K. Curr. Biol. (1996) [Pubmed]
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