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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

HRT1  -  SCF ubiquitin ligase complex subunit HRT1

Saccharomyces cerevisiae S288c

Synonyms: E3 ubiquitin-protein ligase complex SCF subunit HRT1, HRT2, High level expression reduces Ty3 transposition protein 1, RBX1, RING-box protein 1, ...
 
 
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High impact information on HRT1

  • We conclude that Cdc53/Hrt1 comprise a highly conserved module that serves as the functional core of a broad variety of heteromeric ubiquitin ligases [1].
  • Hrt1 shows striking similarity to the Apc11 subunit of anaphase-promoting complex [1].
  • Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase [2].
  • The anaphase delay phenotype can be complemented by ectopic expression of Cul8 but not by any other yeast or human cullins, nor by a cul8 mutant deficient in binding to RING finger protein Roc1 [3].
  • Isolation and characterization of HRT1 using a genetic screen for mutants unable to degrade Gic2p in saccharomyces cerevisiae [4].
 

Biological context of HRT1

  • Finally, Hrt1p was localized in both nucleus and cytoplasm and despite a short half-life was expressed constitutively throughout the cell cycle [4].
  • Cullins, together with the RING-finger protein Rbx1, form the catalytic core of the ligase, and recruit the substrate-recognition module [5].
  • Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis [6].
  • Finally, we report the identification of two SAG family pseudogenes, SAGP1 and SAGP2, that share 36% or 47% sequence identity with ROC1/Rbx1/Hrt1 and 30% or 88% with SAG, respectively [7].
 

Anatomical context of HRT1

  • RESULTS: Caenorhabditis elegans RBX1 is strongly expressed in early embryos and in the gonad, including meiotic cells [6].
  • In humans, both SAG and ROC1 are ubiquitously expressed at a very high level in heart, skeletal muscle, and testis [8].
 

Other interactions of HRT1

  • The SCF family of ubiquitin-ligases consists of a common core machinery, namelySkp1p, Cdc53p, Hrt1p, and a variable component, the F-box protein that is responsible for substrate recognition [9].
 

Analytical, diagnostic and therapeutic context of HRT1

References

  1. Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Seol, J.H., Feldman, R.M., Zachariae, W., Shevchenko, A., Correll, C.C., Lyapina, S., Chi, Y., Galova, M., Claypool, J., Sandmeyer, S., Nasmyth, K., Deshaies, R.J., Shevchenko, A., Deshaies, R.J. Genes Dev. (1999) [Pubmed]
  2. Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. Brower, C.S., Sato, S., Tomomori-Sato, C., Kamura, T., Pause, A., Stearman, R., Klausner, R.D., Malik, S., Lane, W.S., Sorokina, I., Roeder, R.G., Conaway, J.W., Conaway, R.C. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  3. A role for Saccharomyces cerevisiae Cul8 ubiquitin ligase in proper anaphase progression. Michel, J.J., McCarville, J.F., Xiong, Y. J. Biol. Chem. (2003) [Pubmed]
  4. Isolation and characterization of HRT1 using a genetic screen for mutants unable to degrade Gic2p in saccharomyces cerevisiae. Blondel, M., Galan, J.M., Peter, M. Genetics (2000) [Pubmed]
  5. The conserved protein DCN-1/Dcn1p is required for cullin neddylation in C. elegans and S. cerevisiae. Kurz, T., Ozlü, N., Rudolf, F., O'Rourke, S.M., Luke, B., Hofmann, K., Hyman, A.A., Bowerman, B., Peter, M. Nature (2005) [Pubmed]
  6. Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis. Sasagawa, Y., Urano, T., Kohara, Y., Takahashi, H., Higashitani, A. Genes Cells (2003) [Pubmed]
  7. SAG/ROC2/Rbx2/Hrt2, a component of SCF E3 ubiquitin ligase: genomic structure, a splicing variant, and two family pseudogenes. Swaroop, M., Gosink, M., Sun, Y. DNA Cell Biol. (2001) [Pubmed]
  8. SAG/ROC/Rbx/Hrt, a zinc RING finger gene family: molecular cloning, biochemical properties, and biological functions. Sun, Y., Tan, M., Duan, H., Swaroop, M. Antioxid. Redox Signal. (2001) [Pubmed]
  9. Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p. Brunson, L.E., Dixon, C., LeFebvre, A., Sun, L., Mathias, N. Mol. Genet. Genomics (2005) [Pubmed]
  10. Occurrence of a putative SCF ubiquitin ligase complex in Drosophila. Bocca, S.N., Muzzopappa, M., Silberstein, S., Wappner, P. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  11. The conserved RING-H2 finger of ROC1 is required for ubiquitin ligation. Chen, A., Wu, K., Fuchs, S.Y., Tan, P., Gomez, C., Pan, Z.Q. J. Biol. Chem. (2000) [Pubmed]
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