Disease relevance of THI4

• Therefore, the Thi4 protein appears to play an important protective role during heat stress in yeast cells, a feature probably related to the mitochondrial instability and altered oxidative status observed in thi4 mutants [1].

High impact information on THI4

• The expression of CyPBP37 and Thi4p is repressed by thiamine but not by thiazole in the growth medium [2].
• N.crassa CyPBP37 is able to functionally replace Thi4p in yeast thiazole synthesis [2].
• A novel binding protein for a member of CyP40-type Cyclophilins: N.crassa CyPBP37, a growth and thiamine regulated protein homolog to yeast Thi4p [3].
• The C-terminal part of BTH1 showed 53% and 59% sequence similarity to the N-terminal TMP-PPase region of the bifunctional yeast proteins Saccharomyces THI6 and Schizosaccharomyces pombe THI4, respectively [4].
• Conditional thiamine biosynthesis was generated by cloning THI4, a thiamine biosynthetic gene, into a URA3 containing plasmid and transforming a strain disrupted in THI4 [5].

Biological context of THI4

• The mutation point was identified in the conserved motif in 5'-flanking region of these three THI4 homologous genes (ptrA, sti35, and THI4) [6].
• The gene is not essential, and a MOL1 disruption strain showed no apparent phenotype under a variety of growth conditions [7].
• The DNA sequence of the MOL1 gene (for MOLasses-inducible) with its flanking regions was determined (EMBL accession number X61669) [7].
• The 5' region of MOL1 contains the complete sequence previously determined for the SUF4 locus, encoding a tRNA-gly (UCC) gene, which has been mapped to chromosome VII [7].
• Oxidative stress parameters were thus measured in thi4 cells [1].

Associations of THI4 with chemical compounds

• Unlike ST135 of Fusarium, MOL1 is not induced by ethanol or heat shock [7].

Other interactions of THI4

• Firstly, UGP1 was placed under the strongly regulatable THI4 promoter [8].

References