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Gene Review

YUH1  -  Yuh1p

Saccharomyces cerevisiae S288c

Synonyms: J1941, UCH, Ubiquitin carboxyl-terminal hydrolase YUH1, Ubiquitin thioesterase, YJR099W
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Disease relevance of YUH1

  • This strategy, which employs YUH1 and the HIV-1 Pr as tools for the highly selective cleavage of the chimeric substrate, should be applicable to the large-scale production of a variety of peptides [1].
  • As a test, a crude lysate of E. coli expressing a yeast ubiquitin-specific hydrolase (Yuh1) was passed through a Ni-NTA column containing bound HisUb [2].

High impact information on YUH1


Associations of YUH1 with chemical compounds

  • Yeast ubiquitin hydrolase 1 (YUH1), a cysteine protease that catalyzes the removal of ubiquitin C-terminal adducts, is important for the generation of monomeric ubiquitin [6].

Physical interactions of YUH1


Other interactions of YUH1

  • The interaction between the 26 kDa yeast ubiquitin hydrolase (YUH1), involved in maintaining the monomeric ubiquitin pool in cells, and the 8.5 kDa yeast ubiquitin protein has been studied by heteronuclear multidimensional NMR spectroscopy [7].
  • Thus, Yuh1p is the major processing enzyme for Rub1p [8].


  1. Production of chemokines CTAPIII and NAP/2 by digestion of recombinant ubiquitin-CTAPIII with yeast ubiquitin C-terminal hydrolase and human immunodeficiency virus protease. Mildner, A.M., Paddock, D.J., LeCureux, L.W., Leone, J.W., Anderson, D.C., Tomasselli, A.G., Heinrikson, R.L. Protein Expr. Purif. (1999) [Pubmed]
  2. Utility of polyhistidine-tagged ubiquitin in the purification of ubiquitin-protein conjugates and as an affinity ligand for the purification of ubiquitin-specific hydrolases. Beers, E.P., Callis, J. J. Biol. Chem. (1993) [Pubmed]
  3. Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate. Misaghi, S., Galardy, P.J., Meester, W.J., Ovaa, H., Ploegh, H.L., Gaudet, R. J. Biol. Chem. (2005) [Pubmed]
  4. Cloning and functional analysis of the ubiquitin-specific protease gene UBP1 of Saccharomyces cerevisiae. Tobias, J.W., Varshavsky, A. J. Biol. Chem. (1991) [Pubmed]
  5. The use of random-breakage mapping to locate the genes APN1 and YUH1 in the Saccharomyces genome, and to determine gene order near the left end of chromosome XI. Game, J., Bell, M., Ramotar, D., Miller, H. Yeast (1994) [Pubmed]
  6. An NMR analysis of ubiquitin recognition by yeast ubiquitin hydrolase: evidence for novel substrate recognition by a cysteine protease. Sakamoto, T., Tanaka, T., Ito, Y., Rajesh, S., Iwamoto-Sugai, M., Kodera, Y., Tsuchida, N., Shibata, T., Kohno, T. Biochemistry (1999) [Pubmed]
  7. Ubiquitin binding interface mapping on yeast ubiquitin hydrolase by NMR chemical shift perturbation. Rajesh, S., Sakamoto, T., Iwamoto-Sugai, M., Shibata, T., Kohno, T., Ito, Y. Biochemistry (1999) [Pubmed]
  8. Rub1p processing by Yuh1p is required for wild-type levels of Rub1p conjugation to Cdc53p. Linghu, B., Callis, J., Goebl, M.G. Eukaryotic Cell (2002) [Pubmed]
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