Disease relevance of YUH1

• This strategy, which employs YUH1 and the HIV-1 Pr as tools for the highly selective cleavage of the chimeric substrate, should be applicable to the large-scale production of a variety of peptides [1].
• As a test, a crude lysate of E. coli expressing a yeast ubiquitin-specific hydrolase (Yuh1) was passed through a Ni-NTA column containing bound HisUb [2].

High impact information on YUH1

• The structure of the yeast UCH Yuh1-ubiquitin aldehyde complex identified an active site crossover loop predicted to limit the size of suitable substrates [3].
• The amino acid sequence of the 809-residue UBP1 lacks significant similarities to other known proteins, including the 236-residue YUH1 protease [4].
• We have used the previously described technique of random-breakage mapping to locate the two yeast genes APN1 and YUH1 [5].
• The YUH1 locus is located approximately 140 kb from the right telomere of chromosome X, and genetically maps 3.6 cM distal to cdc11 [5].
• The use of random-breakage mapping to locate the genes APN1 and YUH1 in the Saccharomyces genome, and to determine gene order near the left end of chromosome XI [5].

Associations of YUH1 with chemical compounds

• Yeast ubiquitin hydrolase 1 (YUH1), a cysteine protease that catalyzes the removal of ubiquitin C-terminal adducts, is important for the generation of monomeric ubiquitin [6].

Physical interactions of YUH1

• The presence of several hydrophobic clusters in the ubiquitin binding interface of YUH1 suggests that hydrophobic interactions are equally important as ionic interactions in contacting ubiquitin [7].

Other interactions of YUH1

• The interaction between the 26 kDa yeast ubiquitin hydrolase (YUH1), involved in maintaining the monomeric ubiquitin pool in cells, and the 8.5 kDa yeast ubiquitin protein has been studied by heteronuclear multidimensional NMR spectroscopy [7].
• Thus, Yuh1p is the major processing enzyme for Rub1p [8].

References