High impact information on AZF1

• We conclude that AZF1 is a glucose-dependent transcription factor that interacts with the CLN3 A(2)GA(5) repeats to play a positive role in the regulation of CLN3 mRNA expression by glucose [1].
• AZF1 is a glucose-dependent positive regulator of CLN3 transcription in Saccharomyces cerevisiae [1].
• To identify proteins that interact with these elements, we used a 1-hybrid approach, which yielded a nuclear zinc finger protein previously identified as Azf1 [1].
• Therefore the genes are proposed to be named AZF1 (asparagine-rich zinc-finger protein) and PGD1 (polyglutamine domain protein) [2].
• Over-expression of Azf1p in the yeast cell does not influence the expression level of the mitochondrial transcription factor Mtf1p, indicating that the influence of Azf1p on the suppression of the special mitochondrial RNA polymerase mutant is an indirect one [3].

Biological context of AZF1

• We show that glucose-induced expression of a reporter gene driven by the A(2)GA(5) CLN3 regulatory sequences is dependent upon the presence of AZF1 [1].
• These characteristic structural features were used to create the abbreviation AZF1 (Asparagine-rich Zinc Finger protein) [3].
• This approach led to the identification of several candidates, including a putative transcription factor, Azf1p, and Mph1p, a protein with an RNA helicase domain which is known to influence the mutation rate of nuclear and mitochondrial genomes [4].
• Microarray experiments showed that Azf1 activates nonoverlapping gene sets in different carbon sources: in glucose, Azf1 activates carbon and energy metabolism genes, and in glycerol-lactate, Azf1 activates genes needed for cell wall maintenance [5].

Associations of AZF1 with chemical compounds

• Furthermore, deletion of AZF1 markedly reduces the transcriptional induction of CLN3 by glucose [1].
• Gel shift experiments show that Azf1 binds to DNA elements with the sequence AAAAGAAA (A4GA3), a motif enriched in the promoters of Azf1-sensitive genes and predicted by whole-genome studies [5].

Regulatory relationships of AZF1

• Azf1 activates CLN3 transcription in Saccharomyces cerevisiae cells growing in glucose [5].

Analytical, diagnostic and therapeutic context of AZF1

• In this report a specific polyclonal antibody against Azf1p was prepared and used in cell-fractionation experiments and in electron-microscopic studies [3].

References