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Gene Review

ORT1  -  Ort1p

Saccharomyces cerevisiae S288c

Synonyms: ARG11, Mitochondrial ornithine transporter 1, O3299, YOR130C, YOR3299C
 
 
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Disease relevance of ORT1

 

High impact information on ORT1

  • We have used immuno-Western blotting and hemagglutinin epitope-tagged derivatives of Arg11p, Arg8p (a mitochondrial matrix marker), and Arg3p (a cytosolic marker) to demonstrate that Arg11p is confined to the mitochondria and behaves like an integral membrane protein [2].
  • A deletion created in ARG11 causes the same arginine-leaky behavior as the original arg11-1 mutation, which yields a premature stop codon at residue 266 [2].
  • As a working hypothesis, we propose that Arg11p participates in the export of matrix-made ornithine into the cytosol [2].
  • We show that the ARG11 open reading frame encodes a putative 292-residue protein with a predicted molecular mass of 31.5 kDa [2].
  • We describe the identification and functional characterization of two Arabidopsis mitochondrial basic amino acid carriers (BAC), AtmBAC1 and AtmBAC2, which are related to the yeast ornithine (Orn) carrier Ort1p, also known as Arg11p [3].
 

Biological context of ORT1

 

Associations of ORT1 with chemical compounds

  • This emerged from the following observations: (a) the derepressive effect disappears if any extra piece of DNA is inserted upstream from the ARG2 promoter; and (b) similar derepression on low glucose is observed with another yeast promoter (ARG11), provided that the flanking 5' region is short [5].
  • In this work we have characterised the transport of L-arginine and L-ornithine into mitochondria isolated from a wild-type Saccharomyces cerevisiae strain and an isogenic arg11 knock-out mutant [4].
  • AtmBAC1 recognized l-histidine whereas both yeast Ort1p and the mammalian ortholog ORNT1p do not [3].
 

Analytical, diagnostic and therapeutic context of ORT1

  • The present study utilizes site-directed mutagenesis to evaluate the catalytic importance of 7 residues of human prostatic phosphatase, namely His12, His257, Asp258, Arg11, Arg15, Arg54, and Arg79, which are highly conserved in the sequences of high molecular weight acid phosphatases [6].

References

  1. Identification of the yeast ARG-11 gene as a mitochondrial ornithine carrier involved in arginine biosynthesis. Palmieri, L., De Marco, V., Iacobazzi, V., Palmieri, F., Runswick, M.J., Walker, J.E. FEBS Lett. (1997) [Pubmed]
  2. The ARG11 gene of Saccharomyces cerevisiae encodes a mitochondrial integral membrane protein required for arginine biosynthesis. Crabeel, M., Soetens, O., De Rijcke, M., Pratiwi, R., Pankiewicz, R. J. Biol. Chem. (1996) [Pubmed]
  3. Identification of a mitochondrial transporter for basic amino acids in Arabidopsis thaliana by functional reconstitution into liposomes and complementation in yeast. Hoyos, M.E., Palmieri, L., Wertin, T., Arrigoni, R., Polacco, J.C., Palmieri, F. Plant J. (2003) [Pubmed]
  4. Transport of arginine and ornithine into isolated mitochondria of Saccharomyces cerevisiae. Soetens, O., Crabeel, M., El Moualij, B., Duyckaerts, C., Sluse, F. Eur. J. Biochem. (1998) [Pubmed]
  5. A strong carbon source effect is mediated by pUC plasmid sequences in a series of classical yeast vectors designed for promoter characterization. Pauwels, K., Abadjieva, A., Hilven, P., Crabeel, M. Yeast (1999) [Pubmed]
  6. Heterologous expression of human prostatic acid phosphatase and site-directed mutagenesis of the enzyme active site. Ostanin, K., Saeed, A., Van Etten, R.L. J. Biol. Chem. (1994) [Pubmed]
 
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