High impact information on GCR2

• The Rap1 coactivators Gcr1 and Gcr2 form an important bridge between the yeast nuclear pore complex and the transcriptional machinery [1].
• Fairly homologous regions were found between Gcr2 and Gcr1 proteins [2].
• The Gal4 (transcription-activating region)/Gcr1 fusion protein alone complemented the defects of both gcr1 and gcr2 strains [2].
• Role of GCR2 in transcriptional activation of yeast glycolytic genes [2].
• We report the nucleotide sequence of GCR2, which can potentially encode a 58,061-Da protein [2].

Biological context of GCR2

• GCR2 was obtained by complementation from a YCp50 genomic library; the complemented strain had normal enzyme levels, as did a strain with GCR2 in multiple copies [3].
• The E-box DNA binding protein Sgc1p suppresses the gcr2 mutation, which is involved in transcriptional activation of glycolytic genes in Saccharomyces cerevisiae [4].
• gcr2, a new mutation affecting glycolytic gene expression in Saccharomyces cerevisiae [3].
• The conversion of glucose to biomass was higher and, to the contrary, ethanol yield was lower in the gcr2 mutant compared to those in the wild-type strain [5].
• hSGT1 (human suppressor of Gcr two) was isolated as a suppressor gene of the gcr2 mutation [6].

Associations of GCR2 with chemical compounds

• A chromosomal gcr2 null mutant was constructed; its pattern of enzyme activities resembled that of the gcr2-1 mutant and, like the gcr2-1 mutant, its growth defect on glucose was only partial (in contrast to the glucose negativity of the gcr1 mutant) [3].
• We show here that leucine zipper motifs in Gcr1p and Gcr2p (1LZ and 2LZ) are each specific to one of the two activation mechanisms-mutations in 1LZ and 2LZ impair transcription of RP and glycolytic genes, respectively [7].
• A gcr2 null mutant of Saccharomyces cerevisiae grows well on glucose in spite of its lower level of glycolytic enzymes between triose phosphates and pyruvate [8].

Physical interactions of GCR2

• Gcr1p is a DNA binding transcription factor that requires Gcr2p for its functions [9].

Regulatory relationships of GCR2

• Together with use of the two-hybrid system of Fields and Song, they show that Gcr1p in its N-terminal half has a potential transcriptional activating function as well as elements for interaction with Gcr2p, which perhaps acts normally to expose an otherwise cryptic activation domain on Gcr1p [10].

Other interactions of GCR2

• Furthermore, a Rap1/Gcr2 fusion protein partially complemented the defects of gcr1 strains [2].
• Transcription of the HXT4 gene was found to be regulated by Gcr1p and Gcr2p, transcription factors that are required for the regulated high level transcriptions of glycolytic genes [11].

References