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Gene Review

POS5  -  Pos5p

Saccharomyces cerevisiae S288c

Synonyms: NADH kinase POS5, mitochondrial, YPL188W
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Disease relevance of POS5

  • The POS5 gene product was overproduced in Escherichia coli and purified without a mitochondrial targeting sequence [1].
  • Consistent with a role in the mitochondrial response to oxidative stress, a pos5 deletion exhibited a 28-fold increase in oxidative damage to mitochondrial proteins and hypersensitivity to exogenous copper [1].

High impact information on POS5

  • We show here that in Saccharomyces cerevisiae, mitochondrial NADPH is largely provided by the product of the POS5 gene [2].
  • We identified POS5 in a S.cerevisiae genetic screen for hyperoxia-sensitive mutants, or cells that cannot survive in 100% oxygen [2].
  • Pos5p is localized to the mitochondrial matrix of yeast and appears to be important for several NADPH-requiring processes in the mitochondria, including resistance to a broad range of oxidative stress conditions, arginine biosynthesis and mitochondrial iron homeostasis [2].
  • The defect in fermentative growth of utr1 mutants renders POS5 but not POS5-dependent mitochondrial genome maintenance essential because rho-utr1 derivatives are viable [3].
  • Pos5p, existing in the mitochondrial matrix, is critical for higher temperature endurance and mitochondrial functions, such as glycerol usability and arginine biosynthesis [4].

Biological context of POS5

  • Furthermore, disruption of POS5 induced mitochondrial biogenesis as a response to mitochondrial dysfunction [1].
  • Disruption of POS5 increased minus-one frameshift mutations in mitochondrial DNA 50-fold, as measured by the arg8(m) reversion assay, with no increase in nuclear mutations [1].
  • These combined data show that the POS5 NAD/H kinase is an important protein required for a variety of essential cellular pathways and that deficient iron-sulfur cluster assembly may play a critical role in the mitochondrial mutator phenotype observed in the pos5Delta [5].
  • In a search for nuclear genes that affect mutagenesis of mitochondrial DNA in Saccharomyces cerevisiae, an ATP-NAD (NADH) kinase, encoded by POS5, that functions exclusively in mitochondria was identified [1].
  • To understand the molecular mechanism of the mitochondrial genome instability of pos5 mutation we performed gene expression analysis on the pos5Delta [5].

Other interactions of POS5

  • Furthermore, two factors that help maintain mitochondrial GSH in the reduced form, namely the NADH kinase Pos5p and the mitochondrial glutathione reductase (Glr1p), are critical for hyperoxia resistance, whereas their cytosolic counterparts are not [6].


  1. POS5 gene of Saccharomyces cerevisiae encodes a mitochondrial NADH kinase required for stability of mitochondrial DNA. Strand, M.K., Stuart, G.R., Longley, M.J., Graziewicz, M.A., Dominick, O.C., Copeland, W.C. Eukaryotic Cell (2003) [Pubmed]
  2. A novel NADH kinase is the mitochondrial source of NADPH in Saccharomyces cerevisiae. Outten, C.E., Culotta, V.C. EMBO J. (2003) [Pubmed]
  3. Synthetic lethal and biochemical analyses of NAD and NADH kinases in Saccharomyces cerevisiae establish separation of cellular functions. Bieganowski, P., Seidle, H.F., Wojcik, M., Brenner, C. J. Biol. Chem. (2006) [Pubmed]
  4. partial Rescue of pos5 Mutants by YEF1 and UTR1 Genes in Saccharomyces cerevisiae. Li, Y.F., Shi, F. Acta Biochim. Biophys. Sin. (Shanghai) (2006) [Pubmed]
  5. Genomic characterization of POS5, the Saccharomyces cerevisiae mitochondrial NADH kinase. Shianna, K.V., Marchuk, D.A., Strand, M.K. Mitochondrion (2006) [Pubmed]
  6. Cellular factors required for protection from hyperoxia toxicity in Saccharomyces cerevisiae. Outten, C.E., Falk, R.L., Culotta, V.C. Biochem. J. (2005) [Pubmed]
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