Disease relevance of pks12

• Mycobacterium tuberculosis pks12 produces a novel polyketide presented by CD1c to T cells [1].
• The pks12 gene (Maa1979), which may be cotranscribed with a downstream gene (Maa1980), is widely conserved in the actinomycetes [2].

High impact information on pks12

• Inspection of the M. tuberculosis genome identified one candidate gene, pks12, which was predicted to encode the largest protein in M. tuberculosis, consisting of 12 catalytic domains that correspond to key steps in the proposed pathway [1].
• Like pks12 mutants, they exhibited increased Congo red binding, an indirect indication of cell wall modifications [2].
• A pks12 mutant of M. tuberculosis was moderately more susceptible to clarithromycin than was its parent strain; however, susceptibility to ciprofloxacin and penicillin was not altered [2].
• Maa2520 and pks12 are the first genes to be linked by mutation to intrinsic drug resistance in MAC [2].
• The largest open reading frame in the genome of M. tuberculosis H37Rv, pks12, is unique in that it encodes two sets of domains needed to produce fatty acids [3].

Biological context of pks12

• Growth of the pks12 mutant was attenuated in mouse alveolar macrophage cell line MH-S, and the virulence of the mutant in vivo was highly attenuated in a murine model [3].

Analytical, diagnostic and therapeutic context of pks12

• A pks12-disrupted mutant was produced, and disruption was confirmed by both PCR analysis and Southern blotting [3].
• Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MS) and liquid chromatography (LC)-MS analysis of tryptic peptides showed that 54 peptides distributed throughout this protein matched the pks12-encoded sequence [3].

References