Disease relevance of ECs3035

• Purification and properties of cytidine deaminase from escherichia coli [1].

High impact information on ECs3035

• Pyrimidine-requiring cdd mutants of Escherichia coli deficient in cytidine deaminase utilize cytidine as a pyrimidine source by an alternative pathway [2].
• We were also able to observe and characterize time-dependent inhibition of E. coli cytidine deaminase by tetrahydrouridine, 3 [3].
• Compound 1 was designed as a transition-state analog, and is a potent, slow-binding inhibitor of cytidine deaminase (Ashley, G. W., and Bartlett, P. A. (1982) Biochem. Biophys. Res. Commun. 108, 1467-1474) [3].
• ApoB RNA-editing enzyme (APOBEC-1) is a cytidine deaminase [4].
• The observed (15)N kinetic isotope effect of the pyrimidine N-3, for wild-type cytidine deaminase acting on cytidine, is 0.9879, which is consistent with protonation and rehybidization of N-3 with hydroxide ion attack on the adjacent carbon to create a tetrahedral intermediate [5].

Chemical compound and disease context of ECs3035

• 15N isotope effects and solvent deuterium isotope effects have been measured for the hydrolytic deamination of cytidine catalyzed by Escherichia coli cytidine deaminase and for the uncatalyzed reaction proceeding spontaneously in neutral solution at elevated temperatures [5].
• Cytidine deaminase from Escherichia coli is shown here to deaminate 2'-deoxy-3'-thiacytidine enantioselectively to leave 3TC essentially optically pure [6].

Biological context of ECs3035

• Role of glutamate-104 in generating a transition state analogue inhibitor at the active site of cytidine deaminase [7].

Associations of ECs3035 with chemical compounds

• Complementary truncations of a hydrogen bond to ribose involved in transition-state stabilization by cytidine deaminase [8].
• A cdd mutation blocks the pathway by preventing the conversion of deoxycytidine to deoxyuridine, whereas a deoA mutation enhances it by sparing deoxyuridine from catabolism [9].
• Maximum glucose repression of cytidine deaminase and uridine phosphorylase was obtained in correspondence of maximum cytidine induction [10].

Analytical, diagnostic and therapeutic context of ECs3035

• Cytidine deaminase has an apparent molecular weight of 54,000 as determined by gel filtration, whereas sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows a band at molecular weight 35,000 [1].

References