The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

tus  -  inhibitor of replication at Ter, DNA...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1605, JW1602, tau
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of tus


High impact information on tus

  • Insertion of a kanamycin-resistance gene in this open reading frame abolished tus activity [1].
  • We also demonstrated that crude extracts of tus+ cells contain a protein which binds to the T2 terminator sequence [1].
  • Mutations in the tus gene of Escherichia coli, which encodes the replication arrest protein Tus, were isolated using a selection scheme based on the plasmid pHV750T2+, which transforms tus mutants at a much higher frequency than wild type strains [5].
  • Resistance to tus expression mapped to a mutation in the stop codon of the topA gene (topA869), generating an elongated topoisomerase I protein with a marked reduction in activity [6].
  • The inverted Ter sites (designated InvTer::spcr) were initially inserted into the chromosome of a delta tus strain to allow unrestrained chromosomal replication [7].

Biological context of tus

  • The tus gene encodes a DNA-binding protein (Tus) that inhibits replication forks at specific block-sites within the terminus region of the Escherichia coli chromosome [8].
  • In vivo characterization of tus gene expression in Escherichia coli [8].
  • Using primer extension and a promoter fusion to characterize in vivo expression, we have demonstrated that the tus transcription start site is within TerB, and that Tus protein autoregulates expression at this weak promoter [8].
  • The tus gene of Escherichia coli: autoregulation, analysis of flanking sequences and identification of a complementary system in Salmonella typhimurium [2].
  • Although later studies have suggested that fork encounter during termination is an active process involving specific termination sites and the tus protein, the coupling mechanism between termination and cell division remains to be elucidated [9].

Associations of tus with chemical compounds


Analytical, diagnostic and therapeutic context of tus

  • Plasmid DNA was isolated from the tus strain carrying plasmid pOU47 and from a wild-type strain carrying pOU47 in which the right Ter site had been inactivated; in both cases, electron microscopy revealed the presence of multimers as well as rolling-circle structures with double-stranded tails [11].


  1. tus, the trans-acting gene required for termination of DNA replication in Escherichia coli, encodes a DNA-binding protein. Hill, T.M., Tecklenburg, M.L., Pelletier, A.J., Kuempel, P.L. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  2. The tus gene of Escherichia coli: autoregulation, analysis of flanking sequences and identification of a complementary system in Salmonella typhimurium. Roecklein, B., Pelletier, A., Kuempel, P. Res. Microbiol. (1991) [Pubmed]
  3. Polar arrest of the simian virus 40 tumor antigen-mediated replication fork movement in vitro by the tus protein-terB complex of Escherichia coli. Amin, A.A., Hurwitz, J. J. Biol. Chem. (1992) [Pubmed]
  4. Site-directed mutagenesis and phylogenetic comparisons of the Escherichia coli Tus protein: DNA-protein interactions alone can not account for Tus activity. Henderson, T.A., Nilles, A.F., Valjavec-Gratian, M., Hill, T.M. Mol. Genet. Genomics (2001) [Pubmed]
  5. Isolation and characterization of mutants of Tus, the replication arrest protein of Escherichia coli. Skokotas, A., Wrobleski, M., Hill, T.M. J. Biol. Chem. (1994) [Pubmed]
  6. Tus-mediated arrest of DNA replication in Escherichia coli is modulated by DNA supercoiling. Valjavec-Gratian, M., Henderson, T.A., Hill, T.M. Mol. Microbiol. (2005) [Pubmed]
  7. Insertion of inverted Ter sites into the terminus region of the Escherichia coli chromosome delays completion of DNA replication and disrupts the cell cycle. Sharma, B., Hill, T.M. Mol. Microbiol. (1995) [Pubmed]
  8. In vivo characterization of tus gene expression in Escherichia coli. Roecklein, B.A., Kuempel, P.L. Mol. Microbiol. (1992) [Pubmed]
  9. A calcium flux at the termination of replication triggers cell division in Escherichia coli. Hypothesis. Norris, V. Cell Calcium (1989) [Pubmed]
  10. Proline pipe helix: structure of the tus proline repeat determined by 1H NMR. Butcher, D.J., Nedved, M.L., Neiss, T.G., Moe, G.R. Biochemistry (1996) [Pubmed]
  11. Inactivation of the replication-termination system affects the replication mode and causes unstable maintenance of plasmid R1. Krabbe, M., Zabielski, J., Bernander, R., Nordström, K. Mol. Microbiol. (1997) [Pubmed]
WikiGenes - Universities