Disease relevance of fliO

• The partial open reading frame downstream of fliO encodes a putative homolog of both E. coli and S. typhimurium FliP [1].
• Characterization of Pseudomonas aeruginosa fliO, a gene involved in flagellar biosynthesis and adherence [1].
• The range of cell processes regulated by anti-sigma factors is highly varied and includes bacteriophage phage growth, sporulation, stress response, flagellar biosynthesis, pigment production, ion transport, and virulence [2].
• Insertions within temporally regulated genes, such as those involved in flagellar biosynthesis and chemotaxis functions, can now be used directly to monitor transcriptional regulation from Caulobacter promoter sequences [3].
• Flagellar biosynthesis has been studied most thoroughly in laboratory strains of Escherichia coli and Salmonella enterica [4].

High impact information on fliO

• In this issue of Cell, describe the construction of an in silico model for the regulatory network responsible for the control of flagellar biosynthesis in E. coli based on quantitative gene expression data [5].
• We report that flbD, one of the early genes required in trans for expression of these operons, codes for a 52-kDa protein homologous to the transcriptional activators NtrC (NRI), NifA, DctD, HydG, and XylR [6].
• A 1.6-kb KpnI fragment from the PAK chromosome that contained the fliO gene was sequenced [1].
• Several genes up-regulated by BaeR overproduction, including genes for maltose transport, chemotactic responses, and flagellar biosynthesis, required an intact PhoBR or CreBC two-component regulatory system for up-regulation [7].
• Type III secretion functions in flagellar biosynthesis and in export of virulence factors from several animal pathogens, and for plant pathogens, it has been shown to be involved in the export of elicitors of the hypersensitive reaction [8].

Biological context of fliO

• One of the unique genes identified, flbD, demonstrated homology to an ORF from the same operon of Tp [9].
• Most of the identified proteins were shown to be involved in energy metabolism, several cellular molecule biosynthetic pathways and catabolism, cell processes, flagellar biosynthesis and motility, and protein translation and folding [10].

References