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Gene Review:

hisA - N-(5'-phospho-L-ribosyl-formimino)-5-amino...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2019, JW2006

Weil, C.F. et al., Cue, D. et al., Walton, D.A. et al., Gernhardt, P. et al., Pfeiffer, M. et al., et al.

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Disease relevance of hisA

Complementation analysis of three genes, hisA, hisB, and hisG, in Escherichia coli showed that they also were inactive [1].
From a lambda gtWES library of the chromosome of Bacillus subtilis, phages carrying DNA from the hisA and ilv-leu regions were isolated [2].
It carries the structural gene for puromycin transacetylase from Streptomyces alboniger, which is flanked by expression signals of M. voltae structural genes and hisA gene sequences of this bacterium [3].

High impact information on hisA

The sequences upstream of the two hisA genes show only limited sequence homology [4].
A ribosome binding site, G-G-T-G, is located 6 base pairs preceding the ATG translation initiation sequence of both hisA genes [4].
The DNA sequences of a region that includes the hisA gene of two related methanogenic archaebacteria, Methanococcus voltae and Methanococcus vannielii, have been compared [4].
A restriction fragment of Methanococcus thermolithotrophicus genomic DNA was cloned into pUC8 to produce plasmid pET9301, which complements mutations in the hisA gene of Escherichia coli [5].
The ORF (ORF 206) immediately 5' to the hisA gene of M. thermolithotrophicus is partially deleted in the genomes of the two mesophilic species, whereas ORF 114, which is 5' to ORF 206, is conserved in all three species [5].

Biological context of hisA

A single hisA point mutation was found to be a missense mutation [6].
The his phages complemented E. coli his A, B or D mutations and could transform seven mutations in the hisA region of the B. subtilis chromosome; each carried a single EcoR1 insert of about 8.2 kb [2].
Sequencing the DNA (2,155 base pairs) cloned from this thermophilic methanogen demonstrated that the M. thermolithotrophicus hisA gene is located within a cluster of open reading frames (ORFs) and is 68 and 69% homologous at the nucleotide level to the hisA genes of the mesophilic methanococci M. voltae and M. vannielii, respectively [5].

Associations of hisA with chemical compounds

Using a deletion in the hisA gene, a vector was integrated into the chromosome by homologous recombination, thereby reconstituting histidine prototrophy [7].

References

Gene inactivation in Lactococcus lactis: histidine biosynthesis. Delorme, C., Godon, J.J., Ehrlich, S.D., Renault, P. J. Bacteriol. (1993) [Pubmed]
The isolation of lambda phage carrying DNA from the histidine and isoleucine-valine regions of the Bacillus subtilis chromosome. Walton, D.A., Moir, A., Morse, R., Roberts, I., Smith, D.A. J. Gen. Microbiol. (1984) [Pubmed]
Construction of an integration vector for use in the archaebacterium Methanococcus voltae and expression of a eubacterial resistance gene. Gernhardt, P., Possot, O., Foglino, M., Sibold, L., Klein, A. Mol. Gen. Genet. (1990) [Pubmed]
Structure and sequence divergence of two archaebacterial genes. Cue, D., Beckler, G.S., Reeve, J.N., Konisky, J. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
Structure and organization of the hisA gene of the thermophilic archaebacterium Methanococcus thermolithotrophicus. Weil, C.F., Beckler, G.S., Reeve, J.N. J. Bacteriol. (1987) [Pubmed]
DNA sequence analysis of spontaneous histidine mutations in a polA1 strain of Escherichia coli K12 suggests a specific role of the GTGG sequence. Jankovic, M., Kostic, T., Savic, D.J. Mol. Gen. Genet. (1990) [Pubmed]
The vhuU gene encoding a small subunit of a selenium-containing [NiFe]-hydrogenase in Methanococcus voltae appears to be essential for the cell. Pfeiffer, M., Bestgen, H., Bürger, A., Klein, A. Arch. Microbiol. (1998) [Pubmed]

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