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Gene Review

nirB  -  nitrite reductase, large subunit, NAD(P)H...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3353, JW3328
 
 
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Disease relevance of nirB

  • Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome [1].
  • It has been used to direct the high-level expression of heterologous proteins by E. coli strains in fermentors, and attenuated Salmonella strains expressing foreign proteins under nirB promoter (pnir) control have efficiently induced an immune response against these proteins [2].
 

High impact information on nirB

 

Chemical compound and disease context of nirB

 

Biological context of nirB

  • We describe a nirB promoter derivative that allows the cloning of 'cassettes' carrying different FNR-binding sequences and experiments in which a number of point mutations were introduced into these sequences [5].
  • FNR-dependent stimulation of expression from the nirB promoter is critically dependent on the location of the FNR-binding site, and deletion or insertion of one base pair is sufficient to disrupt promoter function [5].
  • The nucleotide sequence at the galP1 CRP binding site located from -31 to -52 displays some similarities with the same region at the nirB promoter [6].
  • From the effects of 13 deletions and three linker-scanner mutations at the Escherichia coli nirB promoter we have located sequences necessary for FNR-dependent induction of activity by anaerobiosis and further nitrite-dependent stimulation of expression [5].
  • Comparison of the nucleotide sequence of the nirB promoter with other promoters that are regulated by FNR show clear homologies, suggesting consensus sequences for FNR binding sites, and confirming that some of the point mutations described here do indeed act by weakening FNR binding [7].
 

Associations of nirB with chemical compounds

  • However, when the nirB gene and its promoter were cloned back upstream from the cysG promoter, the rate of transcription was higher during anaerobic growth than during aerobic growth and was further induced by nitrite [8].
 

Other interactions of nirB

  • No promoter activity was associated with DNA fragments between the BamHI site located within the N-terminal coding region of the nirB gene and the cysG promoter located at the C-terminus of nirC [8].
  • These increases were totally dependent on a functional fnr gene and were shown by S1 mapping experiments to be due to transcriptional read-through from the Fnr-dependent nirB promoter [8].
  • Escherichia coli possesses two distinct nitrite reductase enzymes encoded by the nrfA and nirB operons [9].
  • Unlike the Fnr-dependent fumarate reductase operon, nirB transcription is not repressed by nitrate [10].
 

Analytical, diagnostic and therapeutic context of nirB

References

  1. Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome. Peakman, T., Crouzet, J., Mayaux, J.F., Busby, S., Mohan, S., Harborne, N., Wootton, J., Nicolson, R., Cole, J. Eur. J. Biochem. (1990) [Pubmed]
  2. Studies of the anaerobically induced promoter pnirB and the improved expression of bacterial antigens. Newton, S.M., Klebba, P.E., Hofnung, M., Charbit, A. Res. Microbiol. (1995) [Pubmed]
  3. Definition of nitrite and nitrate response elements at the anaerobically inducible Escherichia coli nirB promoter: interactions between FNR and NarL. Tyson, K.L., Bell, A.I., Cole, J.A., Busby, S.J. Mol. Microbiol. (1993) [Pubmed]
  4. Transcriptional control, translation and function of the products of the five open reading frames of the Escherichia coli nir operon. Harborne, N.R., Griffiths, L., Busby, S.J., Cole, J.A. Mol. Microbiol. (1992) [Pubmed]
  5. Molecular genetic analysis of an FNR-dependent anaerobically inducible Escherichia coli promoter. Bell, A.I., Cole, J.A., Busby, S.J. Mol. Microbiol. (1990) [Pubmed]
  6. The nirB promoter of Escherichia coli: location of nucleotide sequences essential for regulation by oxygen, the FNR protein and nitrite. Jayaraman, P.S., Gaston, K.L., Cole, J.A., Busby, S.J. Mol. Microbiol. (1988) [Pubmed]
  7. Mutational analysis of the nucleotide sequence at the FNR-dependent nirB promoter in Escherichia coli. Jayaraman, P.S., Cole, J.A., Busby, S.J. Nucleic Acids Res. (1989) [Pubmed]
  8. Transcriptional control of the cysG gene of Escherichia coli K-12 during aerobic and anaerobic growth. Peakman, T., Busby, S., Cole, J. Eur. J. Biochem. (1990) [Pubmed]
  9. The nrfA and nirB nitrite reductase operons in Escherichia coli are expressed differently in response to nitrate than to nitrite. Wang, H., Gunsalus, R.P. J. Bacteriol. (2000) [Pubmed]
  10. Lack of redox control of the anaerobically-induced nirB+ gene of Escherichia coli K-12. Griffiths, L., Cole, J.A. Arch. Microbiol. (1987) [Pubmed]
  11. Attenuated Salmonella enterica serovar typhi live vector with inducible chromosomal expression of the T7 RNA polymerase and its evaluation with reporter genes. Santiago-Machuca, A.E., Ruiz-Pérez, F., Delgado-Dominguez, J.S., Becker, I., Isibasi, A., González-Bonilla, C.R. Plasmid (2002) [Pubmed]
 
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