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Gene Review

metE  -  5-methyltetrahydropteroyltriglutamate...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3823, JW3805, metB12
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Disease relevance of metE


High impact information on metE


Chemical compound and disease context of metE


Biological context of metE

  • Three tandem repeats of an 8 bp sequence defined previously as the metE operator site for MetJ-mediated repression also overlap the -35 region of the metR promoter [1].
  • In this study, we show that purified MetR protein also binds to and protects a second 24-bp sequence adjacent to the original site, from nucleotides -24 to -47 relative to the metE transcription initiation site (designated as site 2) [6].
  • Characterization of a second MetR-binding site in the metE metR regulatory region of Salmonella typhimurium [6].
  • Base pair changes in site 1 or site 2 away from the MetR consensus binding sequence resulted in decreased metE-lacZ expression, suggesting that both sites are necessary for expression [6].
  • Via its N-terminal amino acid sequence, the 34-kDa polypeptide was identified as the metE gene product [7].

Associations of metE with chemical compounds

  • The altered regulation of these genes occurs in the presence of high intracellular levels of homocysteine, a methionine pathway intermediate which normally inhibits metH and metR expression and stimulates metE expression [8].
  • The latter two classes of mutations also affected regulation of the metE gene of the folate branch of the methionine pathway, but not metA in the nonfolate branch of the methionine pathway, or the gcv operon, encoding the glycine cleavage enzyme system [9].

Other interactions of metE

  • The DNA-binding protein MetR belongs to the LysR family of transcriptional activators and is required for expression of the metE and metH promoters in Escherichia coli [10].
  • Single and multiple base changes were introduced into sites 1 and 2 in a metE-lacZ fusion [6].


  1. MetJ-mediated regulation of the Salmonella typhimurium metE and metR genes occurs through a common operator region. Wu, W.F., Urbanowski, M.L., Stauffer, G.V. FEMS Microbiol. Lett. (1993) [Pubmed]
  2. Regulation of methionine synthesis in Escherichia coli: effect of the MetR protein on the expression of the metE and metR genes. Maxon, M.E., Redfield, B., Cai, X.Y., Shoeman, R., Fujita, K., Fisher, W., Stauffer, G., Weissbach, H., Brot, N. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  3. Structure-function studies on Escherichia coli MetR protein, a putative prokaryotic leucine zipper protein. Maxon, M.E., Wigboldus, J., Brot, N., Weissbach, H. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  4. Comparison of cobalamin-independent and cobalamin-dependent methionine synthases from Escherichia coli: two solutions to the same chemical problem. González, J.C., Banerjee, R.V., Huang, S., Sumner, J.S., Matthews, R.G. Biochemistry (1992) [Pubmed]
  5. S-methylmethionine metabolism in Escherichia coli. Thanbichler, M., Neuhierl, B., Böck, A. J. Bacteriol. (1999) [Pubmed]
  6. Characterization of a second MetR-binding site in the metE metR regulatory region of Salmonella typhimurium. Wu, W.F., Urbanowski, M.L., Stauffer, G.V. J. Bacteriol. (1995) [Pubmed]
  7. Methylcobalamin:homocysteine methyltransferase from Methanobacterium thermoautotrophicum. Identification as the metE gene product. Schröder, I., Thauer, R.K. Eur. J. Biochem. (1999) [Pubmed]
  8. Escherichia coli metR mutants that produce a MetR activator protein with an altered homocysteine response. Byerly, K.A., Urbanowski, M.L., Stauffer, G.V. J. Bacteriol. (1990) [Pubmed]
  9. Escherichia coli cis- and trans-acting mutations that increase glyA gene expression. Lorenz, E., Plamann, M.D., Stauffer, G.V. Mol. Gen. Genet. (1996) [Pubmed]
  10. A mutation in the rpoA gene encoding the alpha subunit of RNA polymerase that affects metE-metR transcription in Escherichia coli. Jafri, S., Urbanowski, M.L., Stauffer, G.V. J. Bacteriol. (1995) [Pubmed]
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