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Gene Review

metH  -  homocysteine-N5-methyltetrahydrofolate...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK4011, JW3979
 
 
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Disease relevance of metH

  • The iclR gene of Escherichia coli K-12, which encodes a regulatory protein (repressor) for the aceBAK operon, is located between that operon and metH in the 91-min region of the chromosome [1].
  • The first 414 aa of the deduced polypeptide sequence are 92% identical to the 414 aa deduced from the partially sequenced Salmonella typhimurium LT2 metH gene [2].
 

High impact information on metH

  • Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain [3].
  • Maxicell expression of a series of plasmids containing deletions in the metH structural gene was employed to map the position and orientation of the gene on the cloned DNA fragment [3].
  • A synthetic module for the metH gene permits facile mutagenesis of the cobalamin-binding region of Escherichia coli methionine synthase: initial characterization of seven mutant proteins [4].
  • The DNA-binding protein MetR belongs to the LysR family of transcriptional activators and is required for expression of the metE and metH promoters in Escherichia coli [5].
  • On the basis of restriction mapping followed by Southern hybridization experiments, the sor genes were mapped at 91.2 min on the chromosome, 3.3 kbp downstream of the metH-iclR gene cluster, and shown to be transcribed in a counterclockwise direction [6].
 

Chemical compound and disease context of metH

 

Biological context of metH

 

Associations of metH with chemical compounds

  • In order to facilitate studies of the roles of amino acid residues in the cobalamin-binding region of methionine synthase, we have constructed a synthetic module corresponding to nucleotides (nt) 1741-2668 in the metH gene and incorporated it into the wild-type metH gene [4].
  • BACKGROUND: In both mammalian and microbial species, B12-dependent methionine synthase catalyzes methyl transfer from methyltetrahydrofolate (CH3-H4folate) to homocysteine [9].
 

Other interactions of metH

  • The altered regulation of these genes occurs in the presence of high intracellular levels of homocysteine, a methionine pathway intermediate which normally inhibits metH and metR expression and stimulates metE expression [10].
  • In the first class, the mutations caused reduced activation of the metH-lacZ fusions that correlated with reduced MetR binding [7].

References

  1. Overproduction and characterization of the iclR gene product of Escherichia coli K-12 and comparison with that of Salmonella typhimurium LT2. Nègre, D., Cortay, J.C., Old, I.G., Galinier, A., Richaud, C., Saint Girons, I., Cozzone, A.J. Gene (1991) [Pubmed]
  2. Nucleotide sequence of the metH gene of Escherichia coli K-12 and comparison with that of Salmonella typhimurium LT2. Old, I.G., Margarita, D., Glass, R.E., Saint Girons, I. Gene (1990) [Pubmed]
  3. Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain. Banerjee, R.V., Johnston, N.L., Sobeski, J.K., Datta, P., Matthews, R.G. J. Biol. Chem. (1989) [Pubmed]
  4. A synthetic module for the metH gene permits facile mutagenesis of the cobalamin-binding region of Escherichia coli methionine synthase: initial characterization of seven mutant proteins. Amaratunga, M., Fluhr, K., Jarrett, J.T., Drennan, C.L., Ludwig, M.L., Matthews, R.G., Scholten, J.D. Biochemistry (1996) [Pubmed]
  5. A mutation in the rpoA gene encoding the alpha subunit of RNA polymerase that affects metE-metR transcription in Escherichia coli. Jafri, S., Urbanowski, M.L., Stauffer, G.V. J. Bacteriol. (1995) [Pubmed]
  6. Cloning of the Escherichia coli sor genes for L-sorbose transport and metabolism and physical mapping of the genes near metH and iclR. Wehmeier, U.F., Nobelmann, B., Lengeler, J.W. J. Bacteriol. (1992) [Pubmed]
  7. The metR binding site in the Salmonella typhimurium metH gene: DNA sequence constraints on activation. Byerly, K.A., Urbanowski, M.L., Stauffer, G.V. J. Bacteriol. (1991) [Pubmed]
  8. Transcriptional start and MetR binding sites on the Escherichia coli metH gene. Marconi, R., Wigboldus, J., Weissbach, H., Brot, N. Biochem. Biophys. Res. Commun. (1991) [Pubmed]
  9. The structure of the C-terminal domain of methionine synthase: presenting S-adenosylmethionine for reductive methylation of B12. Dixon, M.M., Huang, S., Matthews, R.G., Ludwig, M. Structure (1996) [Pubmed]
  10. Escherichia coli metR mutants that produce a MetR activator protein with an altered homocysteine response. Byerly, K.A., Urbanowski, M.L., Stauffer, G.V. J. Bacteriol. (1990) [Pubmed]
 
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