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Gene Review

PELI1  -  pellino E3 ubiquitin protein ligase 1

Homo sapiens

Synonyms: E3 ubiquitin-protein ligase pellino homolog 1, PRISM, Pellino-1, Pellino-related intracellular-signaling molecule
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Disease relevance of PELI1

  • Methods: Hepatitis C virus genome was detected by real/time detection system using an ABI Prism 7700 sequence detector (Perkin Elmer Corp./Applied Biosystems, Foster City, CA) [1].
  • To investigate the role of viral expression in individuals infected with human T-cell lymphotropic virus type 1 (HTLV-1), a real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) of HTLV-1 tax messenger RNA (mRNA) using ABI Prism 7700 Sequence Detection System was developed [2].
  • We have screened the human genome to localize genetic intervals that may contain lupus susceptibility loci in a sample of 188 lupus patients belonging to 80 lupus families with two or more affected relatives per family using the ABI Prism linkage mapping set which includes 350 polymorphic markers with an average spacing of 12 cM [3].
  • Thirty-six lung cancer cell lines [14 small cell lung cancers (SCLCs) and 22 non-SCLCs (NSCLCs)] and their matched control DNAs were analyzed using 399 fluorescent microsatellite markers from the ABI Prism linkage mapping set v.2 on an ABI 377 sequencer/genotyper [4].
  • Nucleic acids were isolated from these specimens, and two M. tuberculosis molecular targets (mRNA, DNA) were quantified, using the ABI Prism 7700 Sequence Detection System. The Mycobacterium genus-specific 16S rRNA was quantified with a limiting dilution RT-PCR assay [5].

High impact information on PELI1

  • Smad6 negatively regulates interleukin 1-receptor-Toll-like receptor signaling through direct interaction with the adaptor Pellino-1 [6].
  • For the microsatellites, the ABI Prism Linkage Mapping Set version 2, with 402 microsatellite markers, was used, and, for the SNPs, the Early Access Affymetrix Mapping 10K array was used [7].
  • DNA was extracted from blood samples from both patients and controls and was genotyped for the PD-1.3 A/G polymorphism, using an ABI Prism 7900HT Sequence Detection System. RESULTS: The frequency distribution of alleles, carriers, or genotypes did not differ between patients and controls [8].
  • Polymorphisms of the IL1A gene were genotyped by direct sequencing using the ABI Prism 377 Sequence Detection System. The LDSupport program, which was recently developed in our laboratory, was used to estimate the haplotype frequencies of SNPs in the study population [9].
  • These observations allowed us to work out a highly sensitive diagnostic test for the API2-MLT fusion on an ABI Prism 7700 sequence detector that confirmed the results of our initial approach [10].

Chemical compound and disease context of PELI1

  • The added value of minipool and single-donation HBV nucleic acid testing protocols was compared to the currently used Prism (Abbott GmbH & Co. KG) hepatitis B surface antigen (HBsAg) and Auszyme (Abbott GmbH & Co. KG) dynamic HBsAg tests in 15 HBV seroconversion panels [11].
  • Potential new probiotic strains Lactobacillus brevis PELI, L. reuteri ING1, L. rhamnosus VTT E-800 and L. rhamnosus LC-705 were assessed for their adhesion properties using the human intestinal mucus model [12].

Biological context of PELI1

  • Data collection and analysis were performed on the Prism 7700 Sequence Detection System. For comparison with gel electrophoresis methods, each locus was also scored on a subset of 24 samples, using restriction enzymes or single-strand conformational polymorphism (SSCP) [13].
  • Validation of the Applied Biosystems Prism 377 automated sequencer for the forensic short tandem repeat analysis [14].
  • A kinetic quantitative polymerase-chain-reaction (PCR) method, based on fluorescent TaqMan methodology and a new instrument (ABI Prism 7700 Sequence Detection System) capable of measuring fluorescence in real-time, was used to quantify gene amplification in tumor DNA [15].
  • Numerical data collected during electrophoresis of the amplified segment in an ABI Prism 310 Genetic Analyzer were used to calculate the cutoff for allelic imbalance [16].
  • We developed an assay for the detection of the PLP1 gene dosage by real-time quantitative PCR using the ABI Prism 7700 Sequence Detection System and the TaqMan chemistry [17].

Anatomical context of PELI1

  • In this study, a fluorogenic PCR-based assay was developed to quantitatively detect H. hepaticus in mouse ceca and feces using the ABI Prism 7700 sequence detection system [18].
  • Amplified products were detected by an ABI Prism 310 Genetic Analyzer and results were analyzed using GeneScan Analysis Software. RESULTS: A total of 65 amniotic fluid and CVS samples were collected from affected and normal pregnancies [19].
  • Using TaqMan chemistry (Applied Biosystems, Foster City, CA) and the ABI Prism 7700 Sequence Detection System (Applied Biosystems), we validated a large panel of murine and human cytokines, as we as other factors playing a role in the immune system, such a chemokines and apoptotic markers [20].

Associations of PELI1 with chemical compounds

  • The new HBsAg assays were compared to well-established tests (Auszyme Monoclonal [overnight incubation, version B], IMx HBsAg, AxSYM HBsAg, and Prism HBsAg [all from Abbott] and Elecsys HBsAg [Roche Diagnostics]) [21].
  • The HCV RNA molecules were then quantitated by nested competitive reverse transcription-polymerase chain reaction (cRT-PCR) using fluorescein-labeled primers, and analyzed by ABI Prism 310 [22].
  • The specificity of the assay was demonstrated by polyacrylamide gel electrophoresis and ABI Prism BigDye terminator cycle sequencing of the PCR product [23].
  • In five patients (Table I), four males and one female (mean age 60.4 +/- 10.1 years) implanted with the Prism pacemaker, the pacing response to exercise and tilting was assessed before and after the infusion of propranolol [24].
  • The PCR amplified products of these species were directly sequenced using ABI Prism BigDye Terminator Sequencing Kits (Applied Biosystems) [25].

Regulatory relationships of PELI1

  • In support of this hypothesis we show that Pellino proteins induce IRAK-1 polyubiquitination in a RING-dependent manner [26].
  • Whereas the Pellino proteins cause polyubiquitination of IRAK-1, we also show that kinase-active members of the IRAK family (IRAK-1 and IRAK-4) promote reciprocal polyubiquitination of the Pellino proteins and that this is associated with IRAK-induced degradation of the Pellino family [27].

Other interactions of PELI1

  • Mutations in GALK1 were identified by PCR amplification of individual exons and flanking sequences and sequencing using fluorescent terminator technology in an ABI 377 Prism or 3100 automated DNA sequencer [28].
  • To screen for mutations in the EDAR gene, all of its exons and splice junctions were polymerase chain reaction amplified from genomic DNA and sequenced directly in an ABI Prism 310 automated sequencer [29].
  • GSTP1 genotypes were determined using ABI Prism 7700 Sequence Detection System methodology (n=448) and GSTT1 and GSTM1 genotypes using PCR-agarose methodology (n=239) [30].
  • The entire MC4R-gene was amplified by PCR and sequenced on an ABI Prism 3100 automated DNA sequencer [31].
  • A ladder of 24 ACTBP2 (SE33) alleles was separated 175 times by denaturing capillary electrophoresis on an ABI Prism 310 Genetic Analyzer using polymer POP-4 [32].

Analytical, diagnostic and therapeutic context of PELI1

  • METHODS: A minor groove binder-based quantitative real-time PCR assay targeting the B2L gene of parapoxviruses was developed on the ABI Prism and the LightCycler platforms [33].
  • Capillary electrophoresis (CE) instruments, such as the ABI Prism 310 and ABI 3100 Genetic Analyzers, are the method of choice for many laboratories performing STR analysis [34].
  • METHODS: Between September 1997 and June 1998, a whole-body scan and at least 1 tomoscintigram were obtained on 23 occasions in 22 patients (fasting for at least 6 h) using a Prism XP 2000 CDET gamma camera; scanning was begun 45 min after intravenous injection of 150-250 MBq FDG [35].
  • Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis [34].
  • PURPOSE: We describe the capabilities and performance of Prism, an innovative new radiotherapy planning system with unusual features and design [36].


  1. Real-time detection system for quantification of hepatitis C virus genome. Takeuchi, T., Katsume, A., Tanaka, T., Abe, A., Inoue, K., Tsukiyama-Kohara, K., Kawaguchi, R., Tanaka, S., Kohara, M. Gastroenterology (1999) [Pubmed]
  2. Correlation of human T-cell lymphotropic virus type 1 (HTLV-1) mRNA with proviral DNA load, virus-specific CD8(+) T cells, and disease severity in HTLV-1-associated myelopathy (HAM/TSP). Yamano, Y., Nagai, M., Brennan, M., Mora, C.A., Soldan, S.S., Tomaru, U., Takenouchi, N., Izumo, S., Osame, M., Jacobson, S. Blood (2002) [Pubmed]
  3. Genome-wide screen for systemic lupus erythematosus susceptibility genes in multiplex families. Shai, R., Quismorio, F.P., Li, L., Kwon, O.J., Morrison, J., Wallace, D.J., Neuwelt, C.M., Brautbar, C., Gauderman, W.J., Jacob, C.O. Hum. Mol. Genet. (1999) [Pubmed]
  4. Genome-wide allelotyping of lung cancer identifies new regions of allelic loss, differences between small cell lung cancer and non-small cell lung cancer, and loci clustering. Girard, L., Zöchbauer-Müller, S., Virmani, A.K., Gazdar, A.F., Minna, J.D. Cancer Res. (2000) [Pubmed]
  5. Measurement of sputum Mycobacterium tuberculosis messenger RNA as a surrogate for response to chemotherapy. Desjardin, L.E., Perkins, M.D., Wolski, K., Haun, S., Teixeira, L., Chen, Y., Johnson, J.L., Ellner, J.J., Dietze, R., Bates, J., Cave, M.D., Eisenach, K.D. Am. J. Respir. Crit. Care Med. (1999) [Pubmed]
  6. Smad6 negatively regulates interleukin 1-receptor-Toll-like receptor signaling through direct interaction with the adaptor Pellino-1. Choi, K.C., Lee, Y.S., Lim, S., Choi, H.K., Lee, C.H., Lee, E.K., Hong, S., Kim, I.H., Kim, S.J., Park, S.H. Nat. Immunol. (2006) [Pubmed]
  7. Comparison of microsatellites versus single-nucleotide polymorphisms in a genome linkage screen for prostate cancer-susceptibility Loci. Schaid, D.J., Guenther, J.C., Christensen, G.B., Hebbring, S., Rosenow, C., Hilker, C.A., McDonnell, S.K., Cunningham, J.M., Slager, S.L., Blute, M.L., Thibodeau, S.N. Am. J. Hum. Genet. (2004) [Pubmed]
  8. Association of a PDCD1 polymorphism with renal manifestations in systemic lupus erythematosus. Johansson, M., Arlestig, L., Möller, B., Rantapää-Dahlqvist, S. Arthritis Rheum. (2005) [Pubmed]
  9. Association of IL1A gene polymorphisms with susceptibility to and severity of systemic sclerosis in the Japanese population. Kawaguchi, Y., Tochimoto, A., Ichikawa, N., Harigai, M., Hara, M., Kotake, S., Kitamura, Y., Kamatani, N. Arthritis Rheum. (2003) [Pubmed]
  10. The product of the t(11;18), an API2-MLT fusion, marks nearly half of gastric MALT type lymphomas without large cell proliferation. Baens, M., Maes, B., Steyls, A., Geboes, K., Marynen, P., De Wolf-Peeters, C. Am. J. Pathol. (2000) [Pubmed]
  11. Multicenter performance evaluation of a transcription-mediated amplification assay for screening of human immunodeficiency virus-1 RNA, hepatitis C virus RNA, and hepatitis B virus DNA in blood donations. Koppelman, M.H., Assal, A., Chudy, M., Torres, P., de Villaescusa, R.G., Reesink, H.W., Lelie, P.N., Cuypers, H.T. Transfusion (2005) [Pubmed]
  12. Assessment of adhesion properties of novel probiotic strains to human intestinal mucus. Ouwehand, A.C., Tuomola, E.M., Tölkkö, S., Salminen, S. Int. J. Food Microbiol. (2001) [Pubmed]
  13. 5' Nuclease assays for the loci CCR5-+/Delta32, CCR2-V64I, and SDF1-G801A related to pathogenesis of AIDS. Yuan, C.C., Peterson, R.J., Wang, C.D., Goodsaid, F., Waters, D.J. Clin. Chem. (2000) [Pubmed]
  14. Validation of the Applied Biosystems Prism 377 automated sequencer for the forensic short tandem repeat analysis. Frazier, R.R., Millican, E.S., Watson, S.K., Oldroyd, N.J., Sparkles, R.L., Taylor, K.M., Panchal, S., Bark, L., Kimpton, C.P., Gill, P.D. Electrophoresis (1996) [Pubmed]
  15. Novel approach to quantitative polymerase chain reaction using real-time detection: application to the detection of gene amplification in breast cancer. Bièche, I., Olivi, M., Champème, M.H., Vidaud, D., Lidereau, R., Vidaud, M. Int. J. Cancer (1998) [Pubmed]
  16. Multiplex polymerase chain reaction for microsatellite analysis of urine sediment cells: a rapid and inexpensive method for diagnosing and monitoring superficial transitional bladder cell carcinoma. Bartoletti, R., Cai, T., Dal Canto, M., Boddi, V., Nesi, G., Piazzini, M. J. Urol. (2006) [Pubmed]
  17. Diagnosis of Pelizaeus-Merzbacher disease: detection of proteolipid protein gene copy number by real-time PCR. Regis, S., Grossi, S., Lualdi, S., Biancheri, R., Filocamo, M. Neurogenetics (2005) [Pubmed]
  18. Fluorogenic PCR-based quantitative detection of a murine pathogen, Helicobacter hepaticus. Ge, Z., White, D.A., Whary, M.T., Fox, J.G. J. Clin. Microbiol. (2001) [Pubmed]
  19. Rapid prenatal diagnosis of aneuploidy for chromosomes 21, 18, 13, and X by quantitative fluorescence polymerase chain reaction. Ochshorn, Y., Bar-Shira, A., Jonish, A., Yaron, Y. Fetal. Diagn. Ther. (2006) [Pubmed]
  20. The use of real-time reverse transcriptase PCR for the quantification of cytokine gene expression. Overbergh, L., Giulietti, A., Valckx, D., Decallonne, R., Bouillon, R., Mathieu, C. Journal of biomolecular techniques : JBT. (2003) [Pubmed]
  21. Evaluation of two new automated assays for hepatitis B virus surface antigen (HBsAg) detection: IMMULITE HBsAg and IMMULITE 2000 HBsAg. Weber, B., Dengler, T., Berger, A., Doerr, H.W., Rabenau, H. J. Clin. Microbiol. (2003) [Pubmed]
  22. An in vitro system combined with an in-house quantitation assay for screening hepatitis C virus inhibitors. Ho, T.Y., Wu, S.L., Lai, I.L., Cheng, K.S., Kao, S.T., Hsiang, C.Y. Antiviral Res. (2003) [Pubmed]
  23. A novel method for quantitation of human von Willebrand factor messenger RNA. Kerr, R., Stirling, D., Rae, M., White, A.R., Ludlam, C.A. Thromb. Res. (2002) [Pubmed]
  24. Influence of propranolol on the ventricular depolarization gradient. Lasaridis, K., Paul, V.E., Katritsis, D., Ward, D.E., Camm, A.J. Pacing and clinical electrophysiology : PACE. (1991) [Pubmed]
  25. Genetic relatedness of six North-Indian butterfly species (Lepidoptera :Pieridae) based on 16S rRNA sequence analysis. Sobti, R.C., Sharma, V.L., Kumari, M., Gill, T.K., Singh, J., Sodhi, M., Mukesh, M., Bansal, S., Arya, S., Bisnoi, S. Mol. Cell. Biochem. (2007) [Pubmed]
  26. Pellino proteins are more than scaffold proteins in TLR/IL-1R signalling: a role as novel RING E3-ubiquitin-ligases. Schauvliege, R., Janssens, S., Beyaert, R. FEBS Lett. (2006) [Pubmed]
  27. Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for PELLINO proteins being ubiquitin-protein isopeptide ligases. Butler, M.P., Hanly, J.A., Moynagh, P.N. J. Biol. Chem. (2007) [Pubmed]
  28. Galactokinase gene mutations and age-related cataract. Lack of association in an Italian population. Maraini, G., Hejtmancik, J.F., Shiels, A., Mackay, D.S., Aldigeri, R., Jiao, X.D., Williams, S.L., Sperduto, R.D., Reed, G. Mol. Vis. (2003) [Pubmed]
  29. Novel mutations in the EDAR gene in two Pakistani consanguineous families with autosomal recessive hypohidrotic ectodermal dysplasia. Naeem, M., Muhammad, D., Ahmad, W. Br. J. Dermatol. (2005) [Pubmed]
  30. The role of glutathione-S-transferase polymorphisms in ovarian cancer survival. Nagle, C.M., Chenevix-Trench, G., Spurdle, A.B., Webb, P.M. Eur. J. Cancer (2007) [Pubmed]
  31. Melanocortin-4 receptor gene and complications after gastric banding. Peterli, R., Peters, T., von Flüe, M., Hoch, M., Eberle, A.N. Obesity surgery : the official journal of the American Society for Bariatric Surgery and of the Obesity Surgery Society of Australia and New Zealand. (2006) [Pubmed]
  32. Reliability of SE33 typing by capillary electrophoresis. Rothämel, T., Kleemann, W.J., Tröger, H.D. Forensic Sci. Int. (2000) [Pubmed]
  33. Real-time PCR detection of parapoxvirus DNA. Nitsche, A., Büttner, M., Wilhelm, S., Pauli, G., Meyer, H. Clin. Chem. (2006) [Pubmed]
  34. Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis. Butler, J.M., Buel, E., Crivellente, F., McCord, B.R. Electrophoresis (2004) [Pubmed]
  35. Evaluation of FDG uptake by renal malignancies (primary tumor or metastases) using a coincidence detection gamma camera. Montravers, F., Grahek, D., Kerrou, K., Younsi, N., Doublet, J.D., Gattegno, B., Rossert, J., Costa de Beauregard, M.A., Thibault, P., Talbot, J.N. J. Nucl. Med. (2000) [Pubmed]
  36. Prism: a new approach to radiotherapy planning software. Kalet, I.J., Jacky, J.P., Austin-Seymour, M.M., Hummel, S.M., Sullivan, K.J., Unger, J.M. Int. J. Radiat. Oncol. Biol. Phys. (1996) [Pubmed]
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