The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Chemical Compound Review

Firefly luciferin     (2E,4R)-2-(6-oxobenzothiazol- 2-ylidene)-1...

Synonyms: Luciferin, D-Luciferin, AC1NUNCB, CHEMBL443738, CHEBI:17165, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Firefly luciferin

  • Both Cypridina luciferin analog-dependent chemiluminescence and luminol-dependent chemiluminescence were significantly decreased in neutrophils from patients with chronic liver disease (hepatocellular carcinoma < cirrhosis < chronic active hepatitis) when they were compared with normal healthy subjects [1].
  • The activities of antioxidant enzymes and of luciferase in the prothorax (bright) and abdomen (dim) of luminous larval Pyrearinus termitilluminans (Coleoptera: Elateridae) were measured after previous challenge with either hyperoxia, hypoxia, or the firefly luciferase inhibitor luciferin 6'-methyl ether (LME) [2].
  • An intensified charge coupled device video camera was used to noninvasively image luciferase-expressing human prostate tumors and metastases in nude mice, after ip inoculation of luciferin [3].
  • The bioluminescent reaction of adenosine 5'-triphosphate (ATP) with luciferin and luciferase has been used in conjunction with a sensitive photometer (Lab-Line's ATP photometer) to detect significant bacteriuria in urine [4].
  • Overexpression of the apoaequorin cDNA in E. coli now provides the means of obtaining gram quantities of a single isotype of the protein which can be converted to aequorin in the presence of coelenterate luciferin, oxygen and an appropriate thiol [5].

Psychiatry related information on Firefly luciferin


High impact information on Firefly luciferin


Chemical compound and disease context of Firefly luciferin


Biological context of Firefly luciferin

  • Cell viability was measured on the basis of lactate dehydrogenase release, and ATP was quantitated with a luciferin/luciferase assay [13].
  • Furthermore, there were significant positive correlations between albumin/prothrombin time and Cypridina luciferin analog-dependent chemiluminescence/luminol-dependent chemiluminescence [1].
  • Local and systemic induction of two defense-related subtilisin-like protease promoters in transgenic Arabidopsis plants. Luciferin induction of PR gene expression [14].
  • Also, we report that luciferin, the substrate for the reporter luciferase (LUC) gene, is able to activate expression of PR genes, and this may pose a problem when using this gene reporter system in studies related to plant defense [14].
  • These criteria included determination of the efficiency of infection/transfection of various viral and nonviral delivery systems, promoter specificity, and visualization of luciferase activity, and of the ability of luciferin to reach various organs [15].

Anatomical context of Firefly luciferin

  • We measured ATP flow (using the luciferin/luciferase method) through these channels after reconstitution into planar phospholipid membranes [16].
  • At a substrate concentration of 0.01 mM, the peak luminescence from the cos cells expressing luciferase was up to 6-fold greater with the esters than with unmodified luciferin [17].
  • Several measures of energy conservation, namely ADP/O ratio, P/O ratio, ATP/O ratio and phosphorylation detected by continuous assay with purified firefly luciferase and luciferin, all show phosphorylation can occur with mung-bean mitochondria at cyanide concentrations sufficient to inhibit the cytochrome oxidase system [18].
  • Single isolated rat hepatocytes were injected with the ATP-dependent luminescent protein firefly luciferase, and then superfused with 45 microM luciferin in air-equilibrated medium [19].
  • The atractyloside-insensitive accumulation of adenine nucleotides by rat liver mitochondria (as opposed to the exchange-diffusion catalysed by the adenine nucleotide translocase) has been measured by using the luciferin/luciferase assay as well as by measuring [14C]ATP uptake [20].

Associations of Firefly luciferin with other chemical compounds


Gene context of Firefly luciferin


Analytical, diagnostic and therapeutic context of Firefly luciferin


  1. Impaired ability of neutrophils to produce oxygen-derived free radicals in patients with chronic liver disease and hepatocellular carcinoma. Uehara, M., Sato, N. Hepatology (1994) [Pubmed]
  2. Bioluminescence as a possible auxiliary oxygen detoxifying mechanism in elaterid larvae. Barros, M.P., Bechara, E.J. Free Radic. Biol. Med. (1998) [Pubmed]
  3. Combined noninvasive imaging and luminometric quantification of luciferase-labeled human prostate tumors and metastases. El Hilali, N., Rubio, N., Martinez-Villacampa, M., Blanco, J. Lab. Invest. (2002) [Pubmed]
  4. Evaluation of an adenosine 5'-triphosphate assay as a screening method to detect significant bacteriuria. Alexander, D.N., Ederer, G.M., Matsen, J.M. J. Clin. Microbiol. (1976) [Pubmed]
  5. The enzymology and molecular biology of the Ca2+-activated photoprotein, aequorin. Cormier, M.J., Prasher, D.C., Longiaru, M., McCann, R.O. Photochem. Photobiol. (1989) [Pubmed]
  6. Monitoring immediate-early gene expression through firefly luciferase imaging of HRS/J hairless mice. Collaco, A.M., Geusz, M.E. BMC Physiol. (2003) [Pubmed]
  7. H+/ATP ratio of proton transport-coupled ATP synthesis and hydrolysis catalysed by CF0F1-liposomes. Turina, P., Samoray, D., Gräber, P. EMBO J. (2003) [Pubmed]
  8. Can coelenterates make coelenterazine? Dietary requirement for luciferin in cnidarian bioluminescence. Haddock, S.H., Rivers, T.J., Robison, B.H. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  9. Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins. Miesenböck, G., Rothman, J.E. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  10. Cloning and expression of cDNA for the luciferase from the marine ostracod Vargula hilgendorfii. Thompson, E.M., Nagata, S., Tsuji, F.I. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  11. Extraction of Renilla-type luciferin from the calcium-activated photoproteins aequorin, mnemiopsin, and berovin. Ward, W.W., Cormier, M.J. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  12. Cytotoxicity of chloroacetanilide herbicide alachlor in HepG2 cells independent of CYP3A4 and CYP3A7. Miranda, S.R., Meyer, S.A. Food Chem. Toxicol. (2007) [Pubmed]
  13. Mitochondrial dysfunction during anoxia/reoxygenation injury of liver sinusoidal endothelial cells. Fujii, Y., Johnson, M.E., Gores, G.J. Hepatology (1994) [Pubmed]
  14. Local and systemic induction of two defense-related subtilisin-like protease promoters in transgenic Arabidopsis plants. Luciferin induction of PR gene expression. Jordá, L., Vera, P. Plant Physiol. (2000) [Pubmed]
  15. Imaging transgene expression in live animals. Honigman, A., Zeira, E., Ohana, P., Abramovitz, R., Tavor, E., Bar, I., Zilberman, Y., Rabinovsky, R., Gazit, D., Joseph, A., Panet, A., Shai, E., Palmon, A., Laster, M., Galun, E. Mol. Ther. (2001) [Pubmed]
  16. VDAC channels mediate and gate the flow of ATP: implications for the regulation of mitochondrial function. Rostovtseva, T., Colombini, M. Biophys. J. (1997) [Pubmed]
  17. Membrane-permeable luciferin esters for assay of firefly luciferase in live intact cells. Craig, F.F., Simmonds, A.C., Watmore, D., McCapra, F., White, M.R. Biochem. J. (1991) [Pubmed]
  18. Energy conservation by the plant mitochondrial cyanide-insensitive oxidase. Some additional evidence. Wilson, S.B. Biochem. J. (1980) [Pubmed]
  19. Continuous bioluminescent monitoring of cytoplasmic ATP in single isolated rat hepatocytes during metabolic poisoning. Koop, A., Cobbold, P.H. Biochem. J. (1993) [Pubmed]
  20. The transport and accumulation of adenine nucleotides during mitochondrial biogenesis. Pollak, J.K., Sutton, R. Biochem. J. (1980) [Pubmed]
  21. Oxyluciferin, a luminescence product of firefly luciferase, is enzymatically regenerated into luciferin. Gomi, K., Kajiyama, N. J. Biol. Chem. (2001) [Pubmed]
  22. Effect of epalrestat, an aldose reductase inhibitor, on the generation of oxygen-derived free radicals in neutrophils from streptozotocin-induced diabetic rats. Kashima, K., Sato, N., Sato, K., Shimizu, H., Mori, M. Endocrinology (1998) [Pubmed]
  23. Synthesis of dinucleoside tetraphosphates in transfected cells by a firefly luciferase reporter gene. Murphy, G.A., McLennan, A.G. Cell. Mol. Life Sci. (2004) [Pubmed]
  24. Acute production of vascular superoxide by angiotensin II but not by catecholamines. Kawazoe, T., Kosaka, H., Yoneyama, H., Hata, Y. J. Hypertens. (2000) [Pubmed]
  25. High volume bioassays to assess CYP3A4-mediated drug interactions: induction and inhibition in a single cell line. Yueh, M.F., Kawahara, M., Raucy, J. Drug Metab. Dispos. (2005) [Pubmed]
  26. Highly sensitive chemiluminescence method for determining myeloperoxidase in human polymorphonuclear leukocytes. Uehara, K., Hori, K., Nakano, M., Koga, S. Anal. Biochem. (1991) [Pubmed]
  27. Ca2+-induced bioluminescence in Renilla reniformis. Purification and characterization of a calcium-triggered luciferin-binding protein. Charbonneau, H., Cormier, M.J. J. Biol. Chem. (1979) [Pubmed]
  28. Role of a luciferin-binding protein in the circadian bioluminescent reaction of Gonyaulax polyedra. Morse, D., Pappenheimer, A.M., Hastings, J.W. J. Biol. Chem. (1989) [Pubmed]
  29. A novel noninvasive model of endometriosis for monitoring the efficacy of antiangiogenic therapy. Becker, C.M., Wright, R.D., Satchi-Fainaro, R., Funakoshi, T., Folkman, J., Kung, A.L., D'Amato, R.J. Am. J. Pathol. (2006) [Pubmed]
  30. Potentiation by red blood cells of shear-induced platelet aggregation: relative importance of chemical and physical mechanisms. Reimers, R.C., Sutera, S.P., Joist, J.H. Blood (1984) [Pubmed]
  31. Molecular cloning and genomic organization of a gene for luciferin-binding protein from the dinoflagellate Gonyaulax polyedra. Lee, D.H., Mittag, M., Sczekan, S., Morse, D., Hastings, J.W. J. Biol. Chem. (1993) [Pubmed]
  32. Visualization of ATP release in pancreatic acini in response to cholinergic stimulus. Use of fluorescent probes and confocal microscopy. Sorensen, C.E., Novak, I. J. Biol. Chem. (2001) [Pubmed]
  33. Biochemistry of dinoflagellate bioluminescence: purification and characterization of dinoflagellate luciferin from Pyrocystis lunula. Dunlap, J.C., Hastings, J.W. Biochemistry (1981) [Pubmed]
WikiGenes - Universities