High impact information on Orchidaceae

• Applying 10 pmol of okadaic acid (OA), a specific inhibitor of type 1 or type 2A serine/threonine protein phosphatases, to the orchid (Phalaenopsis species) stigma induced a dramatic increase in ethylene production and an accelerated senescence of the whole flower [1].
• The temporal and spatial expression patterns of three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes were investigated in pollinated orchid (Phalaenopsis spp.) flowers [2].
• Identification of phosphoenolpyruvate carboxylase isoforms in leaf, stem and roots of the obligate CAM plant Vanilla planifolia Salib. (Orchidaceae): a physiological and molecular approach [3].
• PeMADS6, a GLOBOSA/PISTILLATA-like gene in Phalaenopsis equestris involved in petaloid formation, and correlated with flower longevity and ovary development [4].
• Transgene integration in transgenic Phalaenopsis lines was confirmed by Southern blot analysis for both CP and pflp genes [5].

Biological context of Orchidaceae

• The monomeric and dimeric mannose-binding proteins from the Orchidaceae species Listera ovata and Epipactis helleborine: sequence homologies and differences in biological activities [6].

Associations of Orchidaceae with chemical compounds

• For this reason, we have studied the pollination syndrome in Phalaenopsis orchids by examining the spatial and temporal location of ethylene biosynthesis within the orchid flower, and how this biosynthesis is regulated by factors that influence expression of genes that encode key enzymes in the ethylene biosynthetic pathway [7].
• Characterization and molecular cloning of mannose-binding lectins from the Orchidaceae species Listera ovata, Epipactis helleborine and Cymbidium hybrid [8].
• 9,10-Dihydrophenanthrenes as phytoalexins of Orchidaceae. Biosynthetic studies in vitro and in vivo proving the route from L-phenylalanine to dihydro-m-coumaric acid, dihydrostilbene and dihydrophenanthrenes [9].
• In young sterile Phalaenopsis plants, the formation of both bibenzyl synthase mRNAs and S-adenosylhomocysteine hydrolase mRNAs was increased upon elicitation more than 100-fold [10].
• Hydroxy derivatives of 9,10-dihydrophenanthrenes, orchinol and hircinol, were isolated from bulbs of Orchidaceae which had been induced to accumulate phytoalexins [9].

Gene context of Orchidaceae

• Four DEF-like MADS box genes displayed distinct floral morphogenetic roles in Phalaenopsis orchid [11].
• Until now, oxalic acid as an acyl moiety of anthocyanins has been reported only in orchid flowers (Orchidaceae) but never in fruits or in the Rosaceae [12].
• Sequence of a cDNA coding for a 1-aminocyclopropane-1-carboxylate synthase homolog from Phalaenopsis [13].
• This method has been tested successfully to clone a Phalaenopsis gene coding for P450 [14].
• Cloning and expression of a putative cytochrome P450 gene that influences the colour of Phalaenopsis flowers [15].

References