Disease relevance of Phenoxazinone

• Grixazone contains a phenoxazinone chromophore and is a secondary metabolite produced by Streptomyces griseus [1].

High impact information on Phenoxazinone

• Recombinant plasmids carrying Streptomyces antibioticus DNA (phenoxazinone synthase plasmids 1.8 and 4.3) have been used as templates in a streptomycete-coupled transcription-translation system [2].
• Activation of phenoxazinone synthase expression in Streptomyces lividans by cloned DNA sequences from Streptomyces antibioticus [2].
• Glycerol-gradient centrifugation of extracts of cells transformed with PHS 1.8 and 4.3 showed that both the large and small phenoxazinone synthase enzyme forms were present [2].
• Cell-free synthesis of the phenoxazinone synthetase subunit by polyribosomes from Streptomyces antibioticus [3].
• UV-vis and (1)H NMR spectroscopy experiments support the phenoxazinone 5/DNA binding [4].

Biological context of Phenoxazinone

• Prevention of growth of human lung carcinoma cells and induction of apoptosis by a novel phenoxazinone, 2-amino-4,4alpha-dihydro-4alpha,7-dimethyl-3H-phenoxazine-3-one [5].
• The structure was confirmed by chemical synthesis through oxidative dimerization of ethyl 3-amino-4-hydroxybenzoate with 2-(N-methylamino)phenol and subsequent hydrolysis of the resultant phenoxazinone ester [6].

Associations of Phenoxazinone with other chemical compounds

• Anti-tumor effects of a novel phenoxazinone, 2-amino-4,4-dihydro-4alpha,7-dimethyl-3H-phenoxazine-3-one (Phx), which was synthesized by the reaction of 2-amino-5-methylphenol with bovine hemoglobin, were studied in terms of suppression of the proliferation of human lung carcinoma cells and apoptosis induction [5].
• Root colonizing microorganisms and the endophytic fungus Fusarium verticillioides participated in benzoxazolinone detoxification by converting the compound to 2-acetamidophenol (AAP) or O-hydroxyphenyl malonamic acid (OHPMA), a process accompanied by the appearance of phenoxazinone [7].

Gene context of Phenoxazinone

• The strain containing the disrupted relA gene did not produce actinomycin and contained significantly lower levels of the enzyme phenoxazinone synthase than the wild-type strain [8].

Analytical, diagnostic and therapeutic context of Phenoxazinone

• Molecular cloning and expression of the phenoxazinone synthase gene from Streptomyces antibioticus [9].
• Truncated fragments of the phenoxazinone synthase gene, phsA, were prepared by the PCR [10].
• Crystallization and initial X-ray analysis of phenoxazinone synthase from Streptomyces antibioticus [11].

References