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Chemical Compound Review

AC1L3TLM     2-amino-4-methyl-N-(4- nitrophenyl)pentanamide

Synonyms: AC1Q5MEV, CTK0H6405, AR-1J9375, AKOS003273846, ST088028, ...
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Disease relevance of L-Leucyl-p-nitroanilide


High impact information on L-Leucyl-p-nitroanilide


Biological context of L-Leucyl-p-nitroanilide


Anatomical context of L-Leucyl-p-nitroanilide


Associations of L-Leucyl-p-nitroanilide with other chemical compounds

  • One specifically hydrolysed N-alpha-benzoyl-DL-arginine-4-nitroanilide and was termed endopeptidase and the other hydrolysed L-lysine- and L-leucine-4-nitroanilide and was termed aminopeptidase [12].

Gene context of L-Leucyl-p-nitroanilide

  • Ten of the M. salivarium and 8 of the M. orale strains were tested for aminopeptidase activity as well, using L-leucine-p-nitroanilide as a substrate, and were shown to possess the activity [13].
  • The Km of APM on rat brain membranes for L-Leucine-p-nitroanilide (0.383 mM) was similar to the Km of purified porcine kidney APM (0.558 mM) [14].
  • 2. The purified enzyme was most active at pH 8.0 with L-leucine-p-nitroanilide as substrate; the Km value for this substrate was 1.1 mmol/l. The microsomal PAP was immunologically different from the pregnancy serum cystyl aminopeptidase (serum PAP) [15].


  1. Not only fibroblasts but also melanoma cells express "leucine aminopeptidase" activity. Borovanský, J., Blasko, M., Siracký, J. Sborník lékar̆ský. (2000) [Pubmed]
  2. The proteolytic enzymes of the K-1 strain of Streptomyces griseus obtained from a commercial preparation (Pronase). Specificity and immobilization of aminopeptidase. Vosbeck, K.D., Greenberg, B.D., Awad, W.M. J. Biol. Chem. (1975) [Pubmed]
  3. Mechanistic studies on the aminopeptidase from Aeromonas proteolytica: a two-metal ion mechanism for peptide hydrolysis. Chen, G., Edwards, T., D'souza, V.M., Holz, R.C. Biochemistry (1997) [Pubmed]
  4. A new technique for detecting oxytocinase activity in electrophoresis gels. Roy, A.C., Saha, N., Tan, S.M., Kamarul, F.Z., Ratnam, S.S. Electrophoresis (1992) [Pubmed]
  5. Modified activity of Aeromonas aminopeptidase: metal ion substitutions and role of substrates. Bayliss, M.E., Prescott, J.M. Biochemistry (1986) [Pubmed]
  6. Arylamidase and cathepsin-A activity of normal and dystrophic human muscle. Kar, N.C., Pearson, C.M. Proc. Soc. Exp. Biol. Med. (1976) [Pubmed]
  7. Oxytocinase activity in human amniotic fluid and its relationship to gestational age. Roy, A.C., Kottegoda, S.R., Viegas, O.A., Ratnam, S.S. Obstetrics and gynecology. (1986) [Pubmed]
  8. The effects of in vitro ageing on the exopeptidases of human diploid fibroblasts. Sambuy, Y., Bittles, A.H. Mech. Ageing Dev. (1984) [Pubmed]
  9. Endogenous cystinyl aminopeptidase in Chinese hamster ovary cells: characterization by [125I]Ang IV binding and catalytic activity. Demaegdt, H., Vanderheyden, P., De Backer, J.P., Mosselmans, S., Laeremans, H., Le, M.T., Kersemans, V., Michotte, Y., Vauquelin, G. Biochem. Pharmacol. (2004) [Pubmed]
  10. Fractionation and characterization of oxytocinases in human semen. Roy, A.C., Sen, D.K., Ratnam, S.S. J. Reprod. Fertil. (1989) [Pubmed]
  11. Purification and enzymatic properties of an L-leucine aminopeptidase from swine liver. Ledeme, N., Hennon, G., Vincent-Fiquet, O., Plaquet, R. Biochim. Biophys. Acta (1981) [Pubmed]
  12. Peptidases from two strains of Pseudomonas fluorescens: partial purification, properties and action on milk. Shamsuzzaman, K., McKellar, R.C. J. Dairy Res. (1987) [Pubmed]
  13. Proteolytic activity of mycoplasmas and ureaplasmas isolated freshly from human saliva. Watanabe, T., Matsuura, M., Seto, K. Med. Microbiol. Immunol. (Berl.) (1985) [Pubmed]
  14. A specific enzyme assay for aminopeptidase M in rat brain. Gillespie, T.J., Konings, P.N., Merrill, B.J., Davis, T.P. Life Sci. (1992) [Pubmed]
  15. Purification and characterization of human placental microsomal aminopeptidase: immunological difference between placental microsomal aminopeptidase and pregnancy serum cystyl-aminopeptidase. Kurauchi, O., Mizutani, S., Okano, K., Narita, O., Tomoda, Y. Enzyme (1986) [Pubmed]
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