Disease relevance of Fussol

• Infection of strain PAOl with phage D3112 results in a 40- to 65-fold increase in the frequency of ami mutants resistant to fluoroacetamide [1].
• The amiE gene encodes an aliphatic amidase capable of converting fluoroacetamide to the toxic compound fluoroacetate and is one of many genes whose expression is subject to catabolite repression control in Pseudomonas aeruginosa [2].
• Acute inhalation toxicity study of 2-fluoroacetamide in rats [3].

High impact information on Fussol

• No biotransformation of 3, 3-dichloropropionic acid was detected, and no GSTZ-catalyzed fluoride release from ethyl fluoroacetate and fluoroacetamide was observed [4].
• The amdS gene was shown to excise spontaneously via the flanking direct repeats when spores were plated onto fluoroacetamide [5].
• We exploited the opportunity for two-way selection in A. niger: transformants can be isolated based on the Amd+ phenotype, whereas counter-selection can be performed using resistance to fluoroacetamide [6].
• In contrast, mutants harboring the crc-10 null allele cannot grow in the presence of fluoroacetamide (due to lack of repression of amiE) [2].
• Selection for succinate-dependent, fluoroacetamide-resistant derivatives of the crc-10 mutant yielded three independent pseudorevertants containing suppressors that restored a degree of catabolite repression control [2].

Biological context of Fussol

• Fluoroacetamide (1081 or F.A.A) is used in Israel for field rodent control [7].

Anatomical context of Fussol

• Fast detection of fluoroacetamide in body fluid using gas chromatography-mass spectrometry after solid-phase microextraction [8].

Associations of Fussol with other chemical compounds

• Visceral tissue levels were found to be approximately 3 mug/g for fluoroacetamide, and 2.5--10 mug/g fluoroacetic acid in guinea pigs and rabbits which had been fatally poisoned with these compounds [9].
• A novel method for fast determination of fluoroacetamide, a kind of organic fluorine pesticide, in blood and urine samples was developed with acetamide as an internal standard using gas chromatography/mass spectrometry (GC/MS) after solid-phase microextraction (SPME) technique [8].
• Glycollamide has wider applicability than fluoroacetamide as a selective agent for obtaining amidase-negative mutants [10].

Gene context of Fussol

• Seven fluoroacetamide-resistant mutants with low amidase activity gave rise to secondary mutant strains on succinate+butyramide plates [11].
• Among mutants of Pseudomonas aeruginosa isolated from fluoroacetamide medium were some which synthesized amidase at about 5% of the rate of the parent constitutive strain, PAC101 [11].

Analytical, diagnostic and therapeutic context of Fussol

• The isolated metabolites were derivatized with N-methyl-N-trimethylsilyltri- fluoroacetamide/trimethyliodosilane and analysed by gas chromatography/mass spectrometry with electron impact (EI) ionization at 70 eV [12].

References