Disease relevance of chlortetracycline

• It appears that the biosynthesis of CTC in Streptomyces aureofaciens takes place at the expense of glycolysis, using up the high-energy phosphate of high-molecular polyphosphates [1].

High impact information on chlortetracycline

• At concentrations up to 10 microM, 12-tetradecanoyl phorbol-13-acetate (TPA) had no effect on the time course of the spontaneous acrosome reaction as scored by the chlortetracycline (CTC) fluorescence assay [2].
• These responses are anteceded by mobilization of membrane-associated calcium, monitored as a decrease in fluorescence of cells preloaded with chlortetracycline (CTC) [3].
• Uncapacitated and capacitated mouse spermatozoa were incubated with specific agonists and antagonists for alpha2-, beta1-, beta2- and beta3-adrenergic receptors for approximately 35 min and then assessed using chlortetracycline (CTC) fluorescence [4].
• However, in low Na+ (25 mmol l-1) medium, monensin could only modulate the transition to the capacitated state, assessed with CTC, indicating that higher concentrations of extracellular Na+ are required for initiation of acrosomal exocytosis [5].
• Following different durations of exposure, spermatozoa were stained with CTC or merocyanine-540, and evaluated with epifluorescent light microscopy or flow cytometry, respectively [6].

Chemical compound and disease context of chlortetracycline

• The activity of ATP-glucokinase and of polyphosphate glucokinase was examined during growth of the actinomycete Streptomyces aureofaciens 8425 under conditions of intense chlortetracycline (CTC) synthesis [1].

Biological context of chlortetracycline

• The area under curve (AUC) was reduced 68% when OTC was given in milk replacer, the reduction of CTC availability was 40% [7].
• The bioavailability of oxytetracycline (OTC) and chlortetracycline (CTC) was studied in non-fasting calves [7].

Associations of chlortetracycline with other chemical compounds

• The availability of OTC was found to be 5% and of CTC 37% after oral administration of 10 mg/kg [7].
• Exposure (30-120 min) to ODF capacitated (p < 0.05) bull spermatozoa as measured by either merocyanine-540 or CTC [6].
• We validate the chlortetracycline (CTC) technique for the evaluation of capacitation and acrosome reaction-like changes in ram sperm, carrying out a double estimation of the acrosome status after treatment with lysophosphatidylcholine, using fluorescein isocyanate (FITC)-RCA/ethidium homodimer 1 (EthD-1) and CTC/EthD-1 [8].

References