High impact information on NSC 96888

• The drug was isolated from plasma by extractive alkylation with pentafluorobenzyl bromide and further derivatized to the bis-trimethylsilyl derivative [1].
• By derivatisation with pentafluorobenzyl bromide, ACC could easily be detected with m/z 280 being the most abundant ion [2].
• The analysis of HETEs involved hydrogenation, solid phase extraction on a C18-cartridge, formation of pentafluorobenzyl bromide and trimethylsilyl ether derivatives [3].
• Following hydrolysis, hexane extraction, and derivatization with pentafluorobenzyl bromide, fatty acid esters are analyzed in two steps: a splitless injection and a second, split injection (1:100) for the quantitation of the more abundant long-chain species [4].
• The method involves extraction with saturated calcium hydroxide solution, esterification with pentafluorobenzyl bromide, clean-up with a silica gel column and determination by capillary GC-SIM [5].

Anatomical context of NSC 96888

• A gas chromatography-mass spectrometry (GC-MS) method is described for the quantification of guanidinoacetate in different body fluids, using a two step derivatisation procedure which involves a reaction with hexafluoroacetylacetone to form a bis(trifluoromethyl)pyrimidine ring structure followed by a reaction with pentafluorobenzyl bromide [6].

Associations of NSC 96888 with other chemical compounds

• The acids are esterified by extractive alkylation, using tetrabutylammonium as a counter ion and pentafluorobenzyl bromide as alkylating reagent [7].
• The plasma is extracted at pH 7.3 with benzene--dichloromethane (9:1), and the residue of the organic extract is reacted at room temperature with pentafluorobenzyl bromide in the presence of 18-crown-6-ether and potassium acetate [8].
• 5. Pentafluorobenzyl derivatives of 5-fluoro- and 5-chlorouracil were prepared by extractive alkylation with pentafluorobenzyl bromide with dichloromethane as solvent and tetrabutylammonium as counter-ion [9].
• Phenobarbital is converted into its bispentafluorobenzyl derivative by extractive alkylation at pH 9 with 0.1 M tetrabutylammonium ion as extracting agent and 0.1 M pentafluorobenzyl bromide as alkylating reagent in methylene chloride [10].
• The evaporated extract was derivatized with pentafluorobenzyl bromide in a water-acetonitrile mixture that was buffered with potassium carbonate, followed by extraction of the derivative into cyclohexane and analysis by gas chromatography-mass spectrometry in electron ionization mode [11].

Gene context of NSC 96888

• The use of pentafluorobenzyl bromide for volatilization of analytes, despite of enhanced ECD response, cannot be recommended for routine analysis [12].

Analytical, diagnostic and therapeutic context of NSC 96888

• The buffered serum is extracted on silica columns, alkylated with pentafluorobenzyl bromide, cleaned up-by extractions and analysed by glass capillary gas chromatography with falling-glass-needle injection and electron-capture detection [13].
• This was achieved by lyophilization of the urine, derivatization with pentafluorobenzyl bromide (PFBBr) and quantification by negative ion chemical ionization GC/MS-MS [14].

References