Disease relevance of AIDS189244

• Effect of 5-methylcytidine on virus production in avian sarcoma virus-infected chicken embryo cells [1].
• Three 5-methylcytidine (m(5)C) bases are found in the rRNAs of Escherichia coli and one of these, at nucleotide 1407 in 16 S rRNA, is the modification product of the methyltransferase (MTase) YebU (also called RsmF) [2].
• The function of the regulatory signal 5-methylcytidine is complex and can be overcome by a number of factors, e.g., by the E1 proteins of adenoviruses [3].

High impact information on AIDS189244

• The rate of transitions at CpG is elevated by an estimated 77-fold, presumably owing to lack of repair of thymidine generated by the spontaneous deamination of 5-methylcytidine [4].
• Antibodies to 5-methylcytidine bound extensively to the DNA of these regions, indicating a high level of DNA methylation [5].
• 5-Methylcytosine has been detected in the DNA of the polytene chromosomes of Sciara coprophila, Drosophila melanogaster and D. persimilis, using specifically purified antibodies to 5-methylcytidine [6].
• In the other six cases, the extra chromosome contained a 5-methylcytidine rich band at each end indicating that both ends were derived from chromosome 15 and contained centromeric heterochromatin normally present on the short arm of chromosome 15 [7].
• Status of DNA global methylation was determined by using DNA methyl acceptance assay and immunohistochemistry staining with 5-methylcytidine antibody [8].

Biological context of AIDS189244

• The marked binding of antibodies specific for 5-methylcytidine to the short arm of chromosome 15 distinguishes this chromosome from the other human acrocentrics [7].
• Our results suggest that although extensively altered tRNA is a poorer substrate than control tRNA in both extent and rate of aminoacylation, 5-methylcytidine in mammalian tRNA is not involved in the recognition of the tRNA by the synthetase as measured by aminoacylation activity [9].

Anatomical context of AIDS189244

• In a human lymphoblastoid cell line (Z83) in which rDNA genes on chromosome 22 are amplified but transcribed at a low level, immunocytological studies with antibodies to 5 methylcytidine provided evidence for hypermethylation of the rDNA [10].
• The study aimed to evaluate the status of global DNA methylation in several types of thyroid tumors using a monoclonal antibody specific for 5-methylcytidine (5-mc) and to define the diagnosis potential of this marker [11].
• The modified base 5-methylcytidine has been found in the DNA of a number of different eukaryotic cells where it occurs principally in the dinucleotide sequence -CmpG- which is present as a palindrome in double-strand nucleic acid molecules [12].

Associations of AIDS189244 with other chemical compounds

• We describe a solid-phase fluoroimmunoassay, based on competition between europium-labeled 5 MeCyd (5-methylcytidine) and sample 5MedCyd (5-methyl-2'-deoxycytidine) for polyclonal anti-5MedCyd antibodies (rabbit) [13].
• To study the role of 5-methylcytidine in the aminoacylation of mammalian tRNA, bulk tRNA specifically deficient in 5-methylcytidine was isolated from the livers of mice treated with 5-azacytidine (18 mg/kg) for 4 days [9].

Gene context of AIDS189244

• Isolation of nucleolar methylase producing only 5-methylcytidine in ribosomal RNA [14].
• 1. The specificity of a monoclonal IgG1 raised against a 5-methylcytidine-keyhole limpet hemocyanin conjugate was investigated by inhibition experiments with soluble competing antigens [15].
• One would like to understand the mechanisms by which 5-methylcytidine residues are capable of interfering with regulatory functions in the late E2A promoter of Ad2 [3].

Analytical, diagnostic and therapeutic context of AIDS189244

• Antibodies specifically directed to 5-methylcytidine were raised in rabbits and purified by affinity chromatography [16].
• Although significant changes in the levels of DNA methylation were detected by HPLC analysis in BrdUrd-treated cells, the dose response for inhibition of pseudomutation by 5-AzaC was correlated with a significant decrease in 5-methylcytidine levels [17].

References