Disease relevance of EDIL3

• To investigate the role of Del1 in tumor growth and angiogenesis, human 143B osteosarcoma cells and murine Lewis lung carcinoma cells were engineered to express Del1 and compared to control transfectants for their ability to produce tumors in nude or syngeneic mice, respectively [1].

High impact information on EDIL3

• Del1 is thus a new ligand for the alphavbeta3 integrin receptor and may function to regulate vascular morphogenesis or remodeling in embryonic development [2].
• Del1 is shown to be a matrix protein and to promote adhesion of endothelial cells through interaction with the alphavbeta3 integrin receptor [2].
• Expression of Del1 protein in the chick chorioallantoic membrane leads to loss of vascular integrity and promotes vessel remodeling [2].
• We have taken advantage of an enhancer trap event in a line of transgenic mice to identify a unique developmentally regulated endothelial cell locus (Del1) [2].
• Embryonic endothelial-like yolk sac cells expressing recombinant Del1 protein, or grown on an extracellular matrix containing Del1 protein, are inhibited from forming vascular-like structures [2].

Biological context of EDIL3

• RESULTS: While no alterations were detected in the exons of EDIL3, several changes were identified in the CSPG2 gene [3].
• Furthermore, Del1 supported VSMC proliferation at least in part by inhibiting these cells from undergoing apoptosis [4].
• Del1 is a unique alpha v beta 3 integrin ligand that is produced by endothelial cells, and thus provides an autocrine signaling pathway in this cell type [1].
• A. blanda dorf-1 constitutes about 0.2% of the genome (3 x 10(4) copies), is bounded by identical long terminal repeats, and exhibits partial homology to the Lilium gypsy-type element del1, but has yet to be confirmed as a retrotransposon [5].
• Nucleotide sequences similar to del1 retrotransposon from Lilium henryi have been discovered in Zea diploperennis as a consequence of finding a Zea retrotransposon element inserted into one of them [6].

Anatomical context of EDIL3

• To determine whether Del1 might signal to other cell types in the vessel wall in a paracrine fashion, studies were conducted with vascular smooth muscle cells (VSMC) [4].
• Adhesion of VSMC to Del1 was associated with organization of actin filaments and formation of focal contacts enriched in vinculin and alpha(v)beta(3) [4].
• Two other degenerins, UNC-8 and DEL-1, are candidate components of a stretch-sensitive channel in motor neurons [7].

Other interactions of EDIL3

• Moreover, notch-related genes, such as Del1 or Del4 (delta-like 1/4) and hey1 or hey2 (hairy/enhancer of split related TRPW motif 1/2), were upregulated in a similar time course [8].

References