Disease relevance of CAPN9

• The expression of both GC36 and nCL-4 was suppressed or depleted in gastric cancer cell lines of differentiated and poorly differentiated types [1].
• Characterization of a human digestive tract-specific calpain, nCL-4, expressed in the baculovirus system [2].
• Purified nCL-4 showed a calcium-dependent activity (calcium concentration at 50% maximum activity (Ka): 0.125 mM) with a sp act of 21 U/mg, which is distinct from those of ubiquitous calpains. nCL-4 exhibited calcium-dependent autolysis, but the cleavage pattern of nCL-4 was clearly different from ubiquitous calpains [2].
• A human glucocerebrosidase cDNA clone was isolated from a human chronic myelogenous leukemia (line K562) cDNA library using a 36-nucleotide-long synthetic probe (GC-36) [3].

Psychiatry related information on CAPN9

• Calpains have been implicated in multiple models of human disease; for instance, calpain 1 is activated in the brains of individuals with Alzheimer's disease, and the digestive tract specific calpain 9 is down-regulated in gastric cancer cell lines [4].

High impact information on CAPN9

• In conclusion, human nCL-4 resembles ubiquitous calpain in some enzymatic properties and interacts with 30K for its activity [2].
• The administration of subcutaneous hCG 36 hours before oocyte retrieval in a natural menstrual cycle [5].
• CONCLUSION(S): The administration of hCG 36 hours before harvesting of immature oocytes may improve the maturational and developmental competence of the oocytes and the pregnancy rates of unstimulated patients with polycystic ovary syndrome [5].
• In combination with maternal age, urine total oestriol had a 32 per cent detection rate at a fixed 5 per cent false-positive rate; urine beta core 34 per cent, urine free beta hCG 36 per cent, maternal serum free beta hCG 44 per cent, and nuchal translucency 82 per cent [6].
• Calpain 10 was recently identified as a susceptibility gene for type 2 diabetes, whereas calpain 9 appears to be a gastric cancer suppressor [7].

Analytical, diagnostic and therapeutic context of CAPN9

• Moreover, a translation product of nCL-4 was detected in rat stomach tissue by immunofluorescence analysis [2].
• Expressed heterodimeric recombinant nCL-4 was purified to near homogeneity by sequential column chromatographies [2].

References